ID A0A4Y8ISJ6_9BACI Unreviewed; 622 AA.
AC A0A4Y8ISJ6;
DT 18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2019, sequence version 1.
DT 28-JAN-2026, entry version 26.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:TFB23110.1};
GN ORFNames=E3U55_04655 {ECO:0000313|EMBL:TFB23110.1};
OS Filobacillus milosensis.
OC Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
OC Filobacillus.
OX NCBI_TaxID=94137 {ECO:0000313|EMBL:TFB23110.1, ECO:0000313|Proteomes:UP000297975};
RN [1] {ECO:0000313|EMBL:TFB23110.1, ECO:0000313|Proteomes:UP000297975}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SH 714 {ECO:0000313|Proteomes:UP000297975};
RA He R.-H.;
RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TFB23110.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; SOPW01000004; TFB23110.1; -; Genomic_DNA.
DR RefSeq; WP_134339183.1; NZ_SOPW01000004.1.
DR AlphaFoldDB; A0A4Y8ISJ6; -.
DR OrthoDB; 9804504at2; -.
DR Proteomes; UP000297975; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR CDD; cd04171; SelB; 1.
DR CDD; cd03696; SelB_II; 1.
DR CDD; cd15491; selB_III; 1.
DR Gene3D; 1.10.10.2770; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR050055; EF-Tu_GTPase.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR015190; Elong_fac_SelB-wing-hlx_typ-2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR005225; Small_GTP-bd.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR NCBIfam; TIGR00475; selB; 1.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR PANTHER; PTHR43721:SF11; SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF09106; WHD_2nd_SelB; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 2.
DR PROSITE; PS51722; G_TR_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Elongation factor {ECO:0000313|EMBL:TFB23110.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000297975}.
FT DOMAIN 3..176
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 622 AA; 70667 MW; BC73DEBE3CFAF3DE CRC64;
MNKHNFTIGM AGHIDHGKTE LVKALTGVET DRLKEEKERK ISIELGYAPL FQNDDMSVSI
IDVPGHERFI RQMIAGVSSI DLTLLVIAAD EGVMPQTIEH LEILNHLDIQ NGLIVITKIR
LADPDLLAVV REEIGDLVKG TVFEGKSIFE VDSINGEGIE QLKTTIQDLL KNITPKPSNG
ALFFPIDQSF HIHGIGTVVR GTIVEGMVKA QEELIILPSQ KKVQVKSIQQ FNEPVQAAYA
GQRAAIALKG IDANEINRGS VVTNNHHAEI TNRVDIELNI SPHLTTVVKQ RTPIKLHTGT
NEVYGKIIFY DRNKLENNED IIYAQLVLDQ TIFVIKDQRF ILRRATPIET IGGGRIIDPY
AEKHKHGEET VVNLKQKAAF DEKDYILSII NNQPGIKLDE LIRLLNYTPE LEKLIKEHYL
VIIDQRLFSV SLINQIKTTI NQYLSEFHLK HPLLLGMNKS ELTSKLPLSN ESNWIIVEDL
ISGKEIIQTQ NFLALPDFKV ELSNGEEQTL SLLLSDLKSD GLKVKEISSY LKHHSLNNKE
DFLIYLLQHE YIHHLQGELY IETNAFKHLI EQLYKKTGAS FSLKEAKDIL DVSRKYLIPF
LEKLDKLNIT KRVENQRYWV NK
//
