ID A0A4Z0D2L5_9FIRM Unreviewed; 333 AA.
AC A0A4Z0D2L5;
DT 18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2019, sequence version 1.
DT 18-JUN-2025, entry version 24.
DE RecName: Full=lipoate--protein ligase {ECO:0000256|ARBA:ARBA00012367};
DE EC=6.3.1.20 {ECO:0000256|ARBA:ARBA00012367};
GN ORFNames=E4100_05705 {ECO:0000313|EMBL:TFZ40000.1};
OS Soehngenia longivitae.
OC Bacteria; Bacillati; Bacillota; Tissierellia; Tissierellales;
OC Tissierellaceae; Soehngenia.
OX NCBI_TaxID=2562294 {ECO:0000313|EMBL:TFZ40000.1, ECO:0000313|Proteomes:UP000298381};
RN [1] {ECO:0000313|EMBL:TFZ40000.1, ECO:0000313|Proteomes:UP000298381}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1933P {ECO:0000313|EMBL:TFZ40000.1,
RC ECO:0000313|Proteomes:UP000298381};
RA Grouzdev D.S., Bidzhieva S.K., Sokolova D.S., Tourova T.P., Poltaraus A.B.,
RA Nazina T.N.;
RT "Draft genome sequence data and analysis of a Fermenting Bacterium,
RT Soehngenia longevitae strain 1933PT, isolated from petroleum reservoir in
RT Azerbaijan.";
RL Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-lysyl-[lipoyl-carrier protein] + (R)-lipoate + ATP = N(6)-
CC [(R)-lipoyl]-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate +
CC H(+); Xref=Rhea:RHEA:49288, Rhea:RHEA-COMP:10500, Rhea:RHEA-
CC COMP:10502, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:83088, ChEBI:CHEBI:83099,
CC ChEBI:CHEBI:456215; EC=6.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00048037};
CC -!- PATHWAY: Protein modification; protein lipoylation via exogenous
CC pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2.
CC {ECO:0000256|ARBA:ARBA00005124}.
CC -!- PATHWAY: Protein modification; protein lipoylation via exogenous
CC pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.
CC {ECO:0000256|ARBA:ARBA00005085}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TFZ40000.1}.
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DR EMBL; SRIB01000007; TFZ40000.1; -; Genomic_DNA.
DR RefSeq; WP_135271072.1; NZ_SRIB01000007.1.
DR AlphaFoldDB; A0A4Z0D2L5; -.
DR OrthoDB; 9788148at2; -.
DR UniPathway; UPA00537; UER00594.
DR Proteomes; UP000298381; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016979; F:lipoate-protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0017118; F:lipoyltransferase activity; IEA:TreeGrafter.
DR GO; GO:0036211; P:protein modification process; IEA:InterPro.
DR CDD; cd16443; LplA; 1.
DR FunFam; 3.30.930.10:FF:000072; Lipoate--protein ligase; 1.
DR Gene3D; 3.30.930.10; Bira Bifunctional Protein, Domain 2; 1.
DR Gene3D; 3.30.390.50; CO dehydrogenase flavoprotein, C-terminal domain; 1.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR004143; BPL_LPL_catalytic.
DR InterPro; IPR019491; Lipoate_protein_ligase_C.
DR InterPro; IPR004562; LipoylTrfase_LipoateP_Ligase.
DR NCBIfam; TIGR00545; lipoyltrans; 1.
DR PANTHER; PTHR12561; LIPOATE-PROTEIN LIGASE; 1.
DR PANTHER; PTHR12561:SF3; LIPOYLTRANSFERASE 1, MITOCHONDRIAL; 1.
DR Pfam; PF10437; Lip_prot_lig_C; 1.
DR Pfam; PF21948; LplA-B_cat; 1.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF82649; SufE/NifU; 1.
DR PROSITE; PS51733; BPL_LPL_CATALYTIC; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:TFZ40000.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000298381}.
FT DOMAIN 26..213
FT /note="BPL/LPL catalytic"
FT /evidence="ECO:0000259|PROSITE:PS51733"
SQ SEQUENCE 333 AA; 38734 MW; 6EA7E0AC164827AA CRC64;
MKFVTNLSNN PYYNLAFEEY LFKNLPDDEE YVFLWINSPA IIVGKNQNTI EEINQQFVND
KDIKVVRRVT GGGAVYHDFG NLNFSIIKNI EGKEKIDFSV INIPILKALE KFGINAELSG
RNDLTVDGKK ISGIAQSLHK RKVLNHGTIL YDTDLSVLSQ ALNVKQDKIE SKGVKSVKSR
VTNIRPYMKE DIDIIEFKEI LLKYIFEYLD QPLEEYKLSD EHLENIDKLC KTKYNTWEWN
YGQSPDFNWK NYKRFPSGSI DVRLEIKSGI IDNIKIYGDF FGTKDVSELE ELLKNTKYEK
NEILNKISKI DIVNYLGNIT QDDFLELLFS YEE
//
