ID A0A5B1BMA3_9FLAO Unreviewed; 914 AA.
AC A0A5B1BMA3;
DT 13-NOV-2019, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2019, sequence version 1.
DT 02-APR-2025, entry version 17.
DE RecName: Full=protein-glutamate O-methyltransferase {ECO:0000256|ARBA:ARBA00012534};
DE EC=2.1.1.80 {ECO:0000256|ARBA:ARBA00012534};
GN ORFNames=F0000_00450 {ECO:0000313|EMBL:KAA1248099.1};
OS Aquimarina sp. RZ0.
OC Bacteria; Pseudomonadati; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Aquimarina.
OX NCBI_TaxID=2607730 {ECO:0000313|EMBL:KAA1248099.1, ECO:0000313|Proteomes:UP000323420};
RN [1] {ECO:0000313|EMBL:KAA1248099.1, ECO:0000313|Proteomes:UP000323420}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RZ0 {ECO:0000313|EMBL:KAA1248099.1,
RC ECO:0000313|Proteomes:UP000323420};
RA Chen X.-Y.;
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-
CC glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:24452, Rhea:RHEA-COMP:10208, Rhea:RHEA-COMP:10311,
CC ChEBI:CHEBI:29973, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:82795; EC=2.1.1.80;
CC Evidence={ECO:0000256|ARBA:ARBA00001541};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAA1248099.1}.
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DR EMBL; VTZU01000001; KAA1248099.1; -; Genomic_DNA.
DR RefSeq; WP_149622720.1; NZ_VTZU01000001.1.
DR AlphaFoldDB; A0A5B1BMA3; -.
DR Proteomes; UP000323420; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
DR GO; GO:0008984; F:protein-glutamate methylesterase activity; IEA:InterPro.
DR GO; GO:0008983; F:protein-glutamate O-methyltransferase activity; IEA:RHEA.
DR GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-UniRule.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd16434; CheB-CheR_fusion; 1.
DR Gene3D; 1.10.155.10; Chemotaxis receptor methyltransferase CheR, N-terminal domain; 1.
DR Gene3D; 3.40.50.180; Methylesterase CheB, C-terminal domain; 1.
DR Gene3D; 3.30.450.20; PAS domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR050903; Bact_Chemotaxis_MeTrfase.
DR InterPro; IPR035909; CheB_C.
DR InterPro; IPR022642; CheR_C.
DR InterPro; IPR000780; CheR_MeTrfase.
DR InterPro; IPR022641; CheR_N.
DR InterPro; IPR036804; CheR_N_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR000673; Sig_transdc_resp-reg_Me-estase.
DR PANTHER; PTHR24422; CHEMOTAXIS PROTEIN METHYLTRANSFERASE; 1.
DR PANTHER; PTHR24422:SF27; PROTEIN-GLUTAMATE O-METHYLTRANSFERASE; 1.
DR Pfam; PF01339; CheB_methylest; 1.
DR Pfam; PF01739; CheR; 1.
DR Pfam; PF03705; CheR_N; 1.
DR Pfam; PF13596; PAS_10; 1.
DR PRINTS; PR00996; CHERMTFRASE.
DR SMART; SM00138; MeTrc; 1.
DR SUPFAM; SSF47757; Chemotaxis receptor methyltransferase CheR, N-terminal domain; 1.
DR SUPFAM; SSF52738; Methylesterase CheB, C-terminal domain; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS50122; CHEB; 1.
DR PROSITE; PS50123; CHER; 1.
PE 4: Predicted;
KW Chemotaxis {ECO:0000256|PROSITE-ProRule:PRU00050};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00050};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000323420};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 24..207
FT /note="CheB-type methylesterase"
FT /evidence="ECO:0000259|PROSITE:PS50122"
FT DOMAIN 225..465
FT /note="CheR-type methyltransferase"
FT /evidence="ECO:0000259|PROSITE:PS50123"
FT COILED 661..741
FT /evidence="ECO:0000256|SAM:Coils"
FT ACT_SITE 30
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT ACT_SITE 57
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
FT ACT_SITE 149
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00050"
SQ SEQUENCE 914 AA; 104956 MW; 566A7F01E6DD9E93 CRC64;
MKNTLTNKID KKKHGDLQNE QFFIIGIGAS AGGLEALKLF FDYVPSDCKH SFVIVQHLSP
DYKSLMVELL AKNTSLPIFE VKNNMVVAPG SIYLIPARKN MKFKNGRLSL TDKPQSHHLN
LPIDIFFHSL AEELKERSIA IILSGTGTDG SRGVHAVKEM GGMVMVQSPA TAKFDGMPNS
AIAIGVVDYI VPVAQMPKEL LDYIATPHSI DTDESVKMLS DEYSLSKILS RIKEHTNLDF
YGYKRPTLIR RIARRMYATQ HKNLKEYYNY IKENDAEVQL LYREFLIGVT KFFRDNDAFQ
ILENKVLPEL VAKKKTDQEF KVWCIGCSTG EEVYSIAILL DEILERKGKK LNVRIFGTDI
EPDNLEKASK AIYSGNITKD VSFDRLQKYF IKKGNQYYVN QKIRNKAIFS CHDVLKDPTI
NKLDLVICRN MLIYINTEMQ QKVLSKIHYA LNKDSILFLG SSETIGDAGN LFNTIDKKWK
LYRTKEIPFR SARDFTRYSD IHKHGLLERS AFKVVARKKP LENNLSEILN ETLLDQFGVA
GIYIDEHYHI MHAVGDFKKY IEIPDDQFSL NLLKMLPENL FIPTSTAIRK VIKQNEKVIY
EGVKWRTKNT IRTINILVNP FELNTTRLNC FLVVFIEEKV EKETATIIKN TLDNPKTAKL
LVAIEEELKL TKESLQTTIE EVETSNEELQ TTNEELLSSN EELQSTNEEL QSVNEELHTV
NAEHQNKIGE LLKLNNDIEN LIQSTHIPTI FLDSDLNIRK FTPNITEQFN LLDTDIGRPI
SHFTSKFGEE DSSNFLQIID RVLGSNKPEQ KEIQISEGKW YLQRIFPFID HSSHNNSGGV
VITFVDINAI KIGQEKMRLK TQELESSNDK LSRFNKAAVG REERMIELKK IINEQCEQLN
REPLFDLNFT KNEL
//
