ID A0A5F5PKV2_HORSE Unreviewed; 2589 AA.
AC A0A5F5PKV2;
DT 11-DEC-2019, integrated into UniProtKB/TrEMBL.
DT 13-SEP-2023, sequence version 2.
DT 28-JAN-2026, entry version 31.
DE RecName: Full=Serine/threonine-protein kinase ATR {ECO:0000256|ARBA:ARBA00024420};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
DE AltName: Full=Ataxia telangiectasia and Rad3-related protein {ECO:0000256|ARBA:ARBA00084048};
GN Name=ATR {ECO:0000313|Ensembl:ENSECAP00000049098.2,
GN ECO:0000313|VGNC:VGNC:15692};
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796 {ECO:0000313|Ensembl:ENSECAP00000049098.2, ECO:0000313|Proteomes:UP000002281};
RN [1] {ECO:0000313|Ensembl:ENSECAP00000049098.2, ECO:0000313|Proteomes:UP000002281}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000049098.2,
RC ECO:0000313|Proteomes:UP000002281};
RX PubMed=19892987; DOI=10.1126/science.1178158;
RG Broad Institute Genome Sequencing Platform;
RG Broad Institute Whole Genome Assembly Team;
RA Wade C.M., Giulotto E., Sigurdsson S., Zoli M., Gnerre S., Imsland F.,
RA Lear T.L., Adelson D.L., Bailey E., Bellone R.R., Bloecker H., Distl O.,
RA Edgar R.C., Garber M., Leeb T., Mauceli E., MacLeod J.N., Penedo M.C.T.,
RA Raison J.M., Sharpe T., Vogel J., Andersson L., Antczak D.F., Biagi T.,
RA Binns M.M., Chowdhary B.P., Coleman S.J., Della Valle G., Fryc S.,
RA Guerin G., Hasegawa T., Hill E.W., Jurka J., Kiialainen A., Lindgren G.,
RA Liu J., Magnani E., Mickelson J.R., Murray J., Nergadze S.G., Onofrio R.,
RA Pedroni S., Piras M.F., Raudsepp T., Rocchi M., Roeed K.H., Ryder O.A.,
RA Searle S., Skow L., Swinburne J.E., Syvaenen A.C., Tozaki T., Valberg S.J.,
RA Vaudin M., White J.R., Zody M.C., Lander E.S., Lindblad-Toh K.;
RT "Genome sequence, comparative analysis, and population genetics of the
RT domestic horse.";
RL Science 326:865-867(2009).
RN [2] {ECO:0000313|Ensembl:ENSECAP00000049098.2}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000049098.2};
RG Ensembl;
RL Submitted (AUG-2025) to UniProtKB.
RN [3] {ECO:0000313|Ensembl:ENSECAP00000049098.2}
RP IDENTIFICATION.
RC STRAIN=Thoroughbred {ECO:0000313|Ensembl:ENSECAP00000049098.2};
RG Ensembl;
RL Submitted (SEP-2025) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP +
CC H(+); Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00048977};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17990;
CC Evidence={ECO:0000256|ARBA:ARBA00048977};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] +
CC ADP + H(+); Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00048659};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:46609;
CC Evidence={ECO:0000256|ARBA:ARBA00048659};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000256|ARBA:ARBA00004286}.
CC Nucleus envelope {ECO:0000256|ARBA:ARBA00004259}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily.
CC {ECO:0000256|ARBA:ARBA00010769}.
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DR Ensembl; ENSECAT00000072220.2; ENSECAP00000049098.2; ENSECAG00000013285.5.
DR VGNC; VGNC:15692; ATR.
DR GeneTree; ENSGT00940000155714; -.
DR Proteomes; UP000002281; Chromosome 16.
DR Bgee; ENSECAG00000013285; Expressed in brainstem and 23 other cell types or tissues.
DR ExpressionAtlas; A0A5F5PKV2; baseline.
DR GO; GO:0070310; C:ATR-ATRIP complex; IEA:UniProtKB-ARBA.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0000077; P:DNA damage checkpoint signaling; IEA:UniProtKB-ARBA.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-ARBA.
DR CDD; cd00892; PIKKc_ATR; 1.
DR FunFam; 1.10.1070.11:FF:000009; Putative serine/threonine-protein kinase ATR; 1.
DR FunFam; 1.25.10.10:FF:000149; Serine/threonine-protein kinase ATR; 1.
DR FunFam; 1.25.40.10:FF:000142; Serine/threonine-protein kinase ATR; 1.
DR FunFam; 3.30.1010.10:FF:000011; serine/threonine-protein kinase ATR; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 2.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR Gene3D; 3.30.1010.10; Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR056802; ATR-like_M-HEAT.
DR InterPro; IPR056803; ATR-like_N-HEAT.
DR InterPro; IPR050517; DDR_Repair_Kinase.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR057564; HEAT_ATR.
DR InterPro; IPR021133; HEAT_type_2.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR012993; UME.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF69; SERINE_THREONINE-PROTEIN KINASE ATR; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF23593; HEAT_ATR; 1.
DR Pfam; PF25030; M-HEAT_ATR; 1.
DR Pfam; PF25032; N-HEAT_ATR; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF08064; UME; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SMART; SM00802; UME; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF48452; TPR-like; 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS50077; HEAT_REPEAT; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Chromosome {ECO:0000256|ARBA:ARBA00022454};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000002281};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT REPEAT 808..842
FT /note="HEAT"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00103"
FT DOMAIN 1643..2133
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 2241..2549
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 2557..2589
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
FT REGION 419..452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 419..429
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2589 AA; 295197 MW; E279BE35C303D23C CRC64;
MGEHGLELAS MIPALRELGS ASPEEYNTVV QKPRQILCQF IDRILTDVNV VALELVKKTD
SQPTSVMLLD FIRHIMKSSP LMFVNVNGSH GQNEAKGSCI EFSNWIITRL LRIAATPSCH
MLHKKICEVI CSLLFLFKSK SPAIFGVLAK ELLYLFEDLI YLHGRNAMGH FVEWPVLVNR
FLSQLDEHVG YLQPAPLQLM NMQNLEFIEV TLLTVLIRII AIVFFRRQEL LLWRIGCVLL
EYGSPKIKSL AISLLTELFE LGGLPAQPAS TFFSSFLQLL KHLVEMDADQ LKVYEEPLSK
LIKTLFPFEA EAYRSIEPVY LNMLLEKLCV MFEDGVLMQL KSDLLKAALC HLLQYFLKYV
PAGYESALPV RKVYVRNICR ALVNVLGVQV DAEYLLGPLY AALKMESMEI IEEVQCQSQQ
ENLSSNSDGI SPKRRRLSSS LNSSKRAPKQ TEAIKHVDMN KKSILWSALK QKAESLQISL
ECSSLKNPVI ETLEGIAVVL QLTALCTVHC SPHNIDCHDF KDCQQKCKKK PSVVITWMSL
DFYTKVLKSC KSLLESVQKP DLEALIDKVV KIFDALIYIQ VKTSFEDQIL EDLCGMLSLP
WIYSCSDDGS LQLTTFATNL LTLSQRIADS YSPQAQSRCV FLLTLFPRRI FLEWRTAVYS
WALQSSHEVI RASCVNGFFI LLQQQNSYNR VPKILIDKVK DDSDIVKKEF ASILGQLVCT
LHGMFYLTSS LTEPFSEHGH TDLFCKSLKV TSQHECSSSR LKASVCKPFL FLLKKKTSSP
VKLAFIDNLC HLCKHLDFRE DETDVKTVLG TLLNLMEDPD KDVRVAFSGN IKHILESLDS
EDGFIKELFV LRMKEAYTHA QISRNNELKD TLILTTGDIG RAAKGDLVPF ALLHLLHCLL
SKSASVSGAA YTEIRALVAA KSVKLQNFFS QYKKPICQFL VESLHSSQMT ALPSTPCQNA
EMRKQDVAHQ REMALNTLSE IANVFDFPDL NRFLTRTLQV LLPDLAAKAS PAASALIRTL
GKQLNVNRRE ILINNFKYIF SHLVCSCSKD ELERALHYLK NETEIELGSL LRQDFQGLHN
ELLLRIGEHY QQVFNGLSIL ASFASSDDPY QGPRDITSPE LMADYLQPKL LGILAFFNMQ
LLSSSVGIED KKMALNSLMS LMKLMGPKHV SSVRVKMMTT LRTGLRFKDD FPELCCRAWD
CFVRCLDHAY LGSLLSHVIV ALLPLIHIQP KETAAIFHYL IIENRDAVQD FLHEIYFLPD
HPELKKIKAV LQEYRKETSE STDLQTTLQL SMKAIQHENV DVRIHALTSL KEILYKNQEK
LIKYATDSET VEPVISQLVT VLLKGCQDAN SQARLLCGEC LGELGAIDPG RLDFSTTETQ
GKDFTFVTGV EDSNFAYGLL MELTRAYLAY ADNSRAQDSA AYAIQELLSI YDCREMQTNG
PGHQLWRRFP EHVREILEPH LNTRYKSSQK STDWSEVKKP IYLSKLGNKF AEWSASWAGY
LITKVRHDLA SKIFTCCSIM MKHDFKVTIY LLPHILVYVL LGCSQEDQQE VYTEIMAVLK
HDDQHIISIQ DSASDLCQLS TQTVFSMLDH LTQWARHKFQ ALNAEKFPQS KSNRDKVDSK
VSTVDYEDYQ SVTRFLDLIP QDTLAVASFR SKAYTRAVMH FESFITEKKQ NIQEHLGFLQ
IIHYHGVVKS MLGLGQLSTV ITQVNGVHAN RSEWTDELNT YRVEAAWKLS QWDLVENYLA
ADGKSTTWSV RLGQLLLSAK KRDATAFYDT LKLVRAEQIV PLSAASFERG SYQRGYEYIV
RLHMLCELEH SIKPLFQQCP GDSSQEDSLN WVARLEMTQN SYRAKEPILA LRRALLSLNK
RPDYNEMVGE CWLQSARVAR KAGHHQTAYN ALLNAGESRL AELYVERAKW LWSKGDVHQA
LIVLQKGVEL CFSENKTPTE SKNMLIHGRA MLLVGRFMEE TANFESNAVM KKYKDVTLFL
PEWEDGHFYL AKYYDKLMPM VTDNKMEKQG DLIRYIVLHF GRSLQYGNQF IYQSMPRMLS
LWLDFGAKAY EWEKASRSDR VQMRNDLAKI NKVITEHTNH LAPYQFLTAF SQLISRICHS
HDEVFVVLME IIAKVFLAYP QQAMWMMTAV SKSSYPMRVN RCKEILNKAI HMKKSLEKFV
GDATRLTDKL LELCNKPVDG SSSTLSMSTH FKMLKKLVEE ATFSEILIPL QSVMIPTLPS
IPGAHANHDP FPGHWAYIAS FDDTVEILAS LQKPKKISLK GSDGKFYIMM CKPKDDLRKD
CRLMEFNSLI NKCLRKDAES RRRELHIRTY AVIPLNDECG IIEWVNNTAG LRPILTKLYK
EKGVYMTGKE LRQCMLPKAA ALSEKLKVFR EFLLPRHPPV FHEWFLRTFP DPTSWYSSRS
AYCRSTAVMS MVGYILGLGD RHGENILFDS LTGECVHVDF NCLFNKGETF EVPEIVPFRL
THNMVNGMGP MGTEGLFRRA CEVTMRLMRD QREPLMSVLK TFLHDPLVEW SKPVKGHSKA
PLNETGEVVN EKAKTHVLDI EQRLQGVIKT RNRVTGLPLS IEGHVHYLIQ EATDENLLCQ
MYLGWTPYM
//
