ID A0A5F8AC46_MACMU Unreviewed; 246 AA.
AC A0A5F8AC46;
DT 11-DEC-2019, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2019, sequence version 1.
DT 18-JUN-2025, entry version 23.
DE RecName: Full=NFU1 iron-sulfur cluster scaffold homolog, mitochondrial {ECO:0000256|ARBA:ARBA00018782};
DE AltName: Full=HIRA-interacting protein 5 {ECO:0000256|ARBA:ARBA00076495};
GN Name=NFU1 {ECO:0000313|Ensembl:ENSMMUP00000074503.1,
GN ECO:0000313|VGNC:VGNC:106188};
OS Macaca mulatta (Rhesus macaque).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9544 {ECO:0000313|Ensembl:ENSMMUP00000074503.1, ECO:0000313|Proteomes:UP000006718};
RN [1] {ECO:0000313|Proteomes:UP000006718}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=17573 {ECO:0000313|Proteomes:UP000006718};
RX PubMed=17431167; DOI=10.1126/science.1139247;
RA Gibbs R.A., Rogers J., Katze M.G., Bumgarner R., Weinstock G.M.,
RA Mardis E.R., Remington K.A., Strausberg R.L., Venter J.C., Wilson R.K.,
RA Batzer M.A., Bustamante C.D., Eichler E.E., Hahn M.W., Hardison R.C.,
RA Makova K.D., Miller W., Milosavljevic A., Palermo R.E., Siepel A.,
RA Sikela J.M., Attaway T., Bell S., Bernard K.E., Buhay C.J.,
RA Chandrabose M.N., Dao M., Davis C., Delehaunty K.D., Ding Y., Dinh H.H.,
RA Dugan-Rocha S., Fulton L.A., Gabisi R.A., Garner T.T., Godfrey J.,
RA Hawes A.C., Hernandez J., Hines S., Holder M., Hume J., Jhangiani S.N.,
RA Joshi V., Khan Z.M., Kirkness E.F., Cree A., Fowler R.G., Lee S.,
RA Lewis L.R., Li Z., Liu Y.-S., Moore S.M., Muzny D., Nazareth L.V.,
RA Ngo D.N., Okwuonu G.O., Pai G., Parker D., Paul H.A., Pfannkoch C.,
RA Pohl C.S., Rogers Y.-H.C., Ruiz S.J., Sabo A., Santibanez J.,
RA Schneider B.W., Smith S.M., Sodergren E., Svatek A.F., Utterback T.R.,
RA Vattathil S., Warren W., White C.S., Chinwalla A.T., Feng Y., Halpern A.L.,
RA Hillier L.W., Huang X., Minx P., Nelson J.O., Pepin K.H., Qin X.,
RA Sutton G.G., Venter E., Walenz B.P., Wallis J.W., Worley K.C., Yang S.-P.,
RA Jones S.M., Marra M.A., Rocchi M., Schein J.E., Baertsch R., Clarke L.,
RA Csuros M., Glasscock J., Harris R.A., Havlak P., Jackson A.R., Jiang H.,
RA Liu Y., Messina D.N., Shen Y., Song H.X.-Z., Wylie T., Zhang L., Birney E.,
RA Han K., Konkel M.K., Lee J., Smit A.F.A., Ullmer B., Wang H., Xing J.,
RA Burhans R., Cheng Z., Karro J.E., Ma J., Raney B., She X., Cox M.J.,
RA Demuth J.P., Dumas L.J., Han S.-G., Hopkins J., Karimpour-Fard A.,
RA Kim Y.H., Pollack J.R., Vinar T., Addo-Quaye C., Degenhardt J., Denby A.,
RA Hubisz M.J., Indap A., Kosiol C., Lahn B.T., Lawson H.A., Marklein A.,
RA Nielsen R., Vallender E.J., Clark A.G., Ferguson B., Hernandez R.D.,
RA Hirani K., Kehrer-Sawatzki H., Kolb J., Patil S., Pu L.-L., Ren Y.,
RA Smith D.G., Wheeler D.A., Schenck I., Ball E.V., Chen R., Cooper D.N.,
RA Giardine B., Hsu F., Kent W.J., Lesk A., Nelson D.L., O'brien W.E.,
RA Pruefer K., Stenson P.D., Wallace J.C., Ke H., Liu X.-M., Wang P.,
RA Xiang A.P., Yang F., Barber G.P., Haussler D., Karolchik D., Kern A.D.,
RA Kuhn R.M., Smith K.E., Zwieg A.S.;
RT "Evolutionary and biomedical insights from the rhesus macaque genome.";
RL Science 316:222-234(2007).
RN [2] {ECO:0000313|Ensembl:ENSMMUP00000074503.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=17573 {ECO:0000313|Ensembl:ENSMMUP00000074503.1};
RA Dutcher S., Fulton R., Lindsay T.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Ensembl:ENSMMUP00000074503.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=17573 {ECO:0000313|Ensembl:ENSMMUP00000074503.1};
RA Graves T., Eichler E.E., Wilson R.K.;
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|Ensembl:ENSMMUP00000074503.1}
RP IDENTIFICATION.
RC STRAIN=17573 {ECO:0000313|Ensembl:ENSMMUP00000074503.1};
RG Ensembl;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- FUNCTION: Iron-sulfur cluster scaffold protein which can assemble [4Fe-
CC 4S] clusters and deliver them to target proteins.
CC {ECO:0000256|ARBA:ARBA00057718}.
CC -!- SUBUNIT: Monomer and homohexamer; the apo-NFU1 is a monomer, while the
CC holo-NFU1 is a hexamer composed of a trimer of dimer that is probably
CC linked by some 4Fe-4S cluster. Interacts with HIRA and EPM2A/laforin.
CC Interacts with BOLA3. Interacts with HSPA9.
CC {ECO:0000256|ARBA:ARBA00062506}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}.
CC -!- SIMILARITY: Belongs to the NifU family.
CC {ECO:0000256|ARBA:ARBA00006420}.
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DR RefSeq; XP_014968082.2; XM_015112596.2.
DR AlphaFoldDB; A0A5F8AC46; -.
DR SMR; A0A5F8AC46; -.
DR FunCoup; A0A5F8AC46; 2343.
DR STRING; 9544.ENSMMUP00000074503; -.
DR Ensembl; ENSMMUT00000081374.1; ENSMMUP00000074503.1; ENSMMUG00000059210.1.
DR GeneID; 707942; -.
DR VEuPathDB; HostDB:ENSMMUG00000059210; -.
DR VGNC; VGNC:106188; NFU1.
DR GeneTree; ENSGT00390000011296; -.
DR InParanoid; A0A5F8AC46; -.
DR OMA; AIMEHYM; -.
DR Proteomes; UP000006718; Chromosome 13.
DR Bgee; ENSMMUG00000059210; Expressed in adipose tissue and 21 other cell types or tissues.
DR ExpressionAtlas; A0A5F8AC46; baseline.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:Ensembl.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IEA:Ensembl.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:Ensembl.
DR GO; GO:0051604; P:protein maturation; IEA:Ensembl.
DR FunFam; 3.30.300.130:FF:000001; NFU1 iron-sulfur cluster scaffold; 1.
DR FunFam; 3.30.1370.70:FF:000002; NFU1 iron-sulfur cluster scaffold homolog, mitochondrial; 1.
DR Gene3D; 3.30.300.130; Fe-S cluster assembly (FSCA); 1.
DR Gene3D; 3.30.1370.70; Scaffold protein Nfu/NifU, N-terminal domain; 1.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR014824; Nfu/NifU_N.
DR InterPro; IPR036498; Nfu/NifU_N_sf.
DR InterPro; IPR035433; NFU1-like.
DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR PANTHER; PTHR11178; IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED; 1.
DR PANTHER; PTHR11178:SF1; NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL; 1.
DR Pfam; PF08712; Nfu_N; 1.
DR Pfam; PF01106; NifU; 1.
DR PIRSF; PIRSF036773; HIRIP5; 1.
DR SMART; SM00932; Nfu_N; 1.
DR SUPFAM; SSF117916; Fe-S cluster assembly (FSCA) domain-like; 1.
DR SUPFAM; SSF110836; Hypothetical protein SAV1430; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00023014};
KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW Reference proteome {ECO:0000313|Proteomes:UP000006718};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 53..140
FT /note="Scaffold protein Nfu/NifU N-terminal"
FT /evidence="ECO:0000259|SMART:SM00932"
SQ SEQUENCE 246 AA; 27640 MW; 958A63B18238826C CRC64;
MEPAGPPCRR SWGFCHMLKN PYTIKKQPLH QFVQRPLFPL PAAFCNPVRY MFIQTQDTPN
PNSLKFIPGK PVLETRTMDF PTPAAAFRSP LARQLFRIEG VKSVFFGPDF ITVTKENEDL
DWNLLKPDIY ATIMDFFASG LPLVTEETPS GEAGSEEDDE VVAMIKELLD TRIRPTVQED
GGDVIYKGFE DGIVQLKLQG SCTSCPSSII TLKNGIQNML QFYIPEVEGV EQVMDDESDE
KEANSP
//
