ID   A0A5J4JKN2_9BACI        Unreviewed;       721 AA.
AC   A0A5J4JKN2;
DT   11-DEC-2019, integrated into UniProtKB/TrEMBL.
DT   11-DEC-2019, sequence version 1.
DT   28-JAN-2026, entry version 18.
DE   RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE            EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE   AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE   AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE   AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE   AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN   ORFNames=BpJC7_22200 {ECO:0000313|EMBL:GER70917.1};
OS   Weizmannia acidilactici.
OC   Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
OC   Heyndrickxia.
OX   NCBI_TaxID=2607726 {ECO:0000313|EMBL:GER70917.1, ECO:0000313|Proteomes:UP000391919};
RN   [1] {ECO:0000313|EMBL:GER70917.1, ECO:0000313|Proteomes:UP000391919}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JC-7 {ECO:0000313|EMBL:GER70917.1,
RC   ECO:0000313|Proteomes:UP000391919};
RA   Tanaka N., Shiwa Y., Fujita N., Tanasupawat S.;
RT   "Draft genome sequence of Bacillus sp. JC-7.";
RL   Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC         passage and rejoining.; EC=5.6.2.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000213};
CC   -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC       {ECO:0000256|ARBA:ARBA00009446}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:GER70917.1}.
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DR   EMBL; BKZQ01000031; GER70917.1; -; Genomic_DNA.
DR   RefSeq; WP_151680938.1; NZ_BKZP01000032.1.
DR   AlphaFoldDB; A0A5J4JKN2; -.
DR   Proteomes; UP000391919; Unassembled WGS sequence.
DR   GO; GO:0043597; C:cytoplasmic replication fork; IEA:TreeGrafter.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:TreeGrafter.
DR   GO; GO:0006281; P:DNA repair; IEA:TreeGrafter.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   CDD; cd00186; TOP1Ac; 1.
DR   CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR   Gene3D; 3.40.50.140; -; 1.
DR   Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR   Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR   Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR   InterPro; IPR000380; Topo_IA.
DR   InterPro; IPR003601; Topo_IA_2.
DR   InterPro; IPR023406; Topo_IA_AS.
DR   InterPro; IPR013497; Topo_IA_cen.
DR   InterPro; IPR013824; Topo_IA_cen_sub1.
DR   InterPro; IPR013825; Topo_IA_cen_sub2.
DR   InterPro; IPR013826; Topo_IA_cen_sub3.
DR   InterPro; IPR023405; Topo_IA_core_domain.
DR   InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR   InterPro; IPR005738; TopoIII.
DR   InterPro; IPR025589; Toprim_C_rpt.
DR   InterPro; IPR006171; TOPRIM_dom.
DR   InterPro; IPR034144; TOPRIM_TopoIII.
DR   NCBIfam; NF005829; PRK07726.1; 1.
DR   NCBIfam; TIGR01056; topB; 1.
DR   PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR   PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR   Pfam; PF01131; Topoisom_bac; 1.
DR   Pfam; PF01751; Toprim; 1.
DR   Pfam; PF13342; Toprim_Crpt; 1.
DR   PRINTS; PR00417; PRTPISMRASEI.
DR   SMART; SM00437; TOP1Ac; 1.
DR   SMART; SM00436; TOP1Bc; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR   PROSITE; PS00396; TOPO_IA_1; 1.
DR   PROSITE; PS52039; TOPO_IA_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000391919};
KW   Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT   DOMAIN          159..599
FT                   /note="Topo IA-type catalytic"
FT                   /evidence="ECO:0000259|PROSITE:PS52039"
SQ   SEQUENCE   721 AA;  81871 MW;  793E06BE4662F085 CRC64;
     MSFPLIIAEK PDQGLTLCSV FPFVKRQGYI EIRPNDIFPE GAYCTWAVGH LAQLVQPESY
     NPAWKQWKLE TLPIIPEKFR YEVSKTKYKQ FHTVKKLLKD ARVTEIIHAG DAGREGELII
     RNIIHLSGIK KPLKRLWISS LTPQSIKEGF RNLLHDEDTS SLYHEAYARA CADWLVGMNA
     SRVYSLLLKQ KGINDVFSVG RVQTPTLALI VKRELEIENF KPEPFWEVTA LFQFDDKQYE
     GKWVKNGESR LKDPAMAERI AAFCRQKPAK VAALKKERKE FQPPLLFNLS SLQAAANQAI
     KFPPKKTLDL VQSLYQKGIV SYPRSDSNYV TKGEAETFPD ILQKLQTYPE YAPFFPLPHP
     SIINNKRFVN EKKVSDHYAI IPTEQVVNPE NLPNDERKIY DMIVRRLIAA HYGNAVYHYT
     TVKTLVDGRA EFISKGTQME KEGWRKVIFA NEKEKDRILP EVKQGDKGIV KDVKIKEGKT
     QPPKRYTEGQ LITLMKTAGK FIEDEALEKV MADVEGLGTE ATRAGVITLL KERKYIEVKK
     NQVYATDKAK VLIRAVGGKI LASPEMTAKW EQRLREIGKG QASPAGFMEQ SKKLSAKIVH
     DAVQSANEWD FSGLKVDEIK RVKSKYTLGK KMGKCPKCGG DIVDKGNFYG CSAYQKSGCT
     FTLPKVILGK KISQANVKKL LAGEETNLIK GFKKNEKAFD AKLKLEQEKL RFIFGKEKTE
     G
//