ID A0A5J4N7C1_9TREM Unreviewed; 1633 AA.
AC A0A5J4N7C1;
DT 11-DEC-2019, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2019, sequence version 1.
DT 18-JUN-2025, entry version 16.
DE RecName: Full=Pre-mRNA-processing-splicing factor 8 {ECO:0000256|ARBA:ARBA00071757};
DE AltName: Full=Splicing factor Prp8 {ECO:0000256|ARBA:ARBA00079260};
GN ORFNames=DEA37_0011608 {ECO:0000313|EMBL:KAA3671445.1};
OS Paragonimus westermani.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda;
OC Digenea; Plagiorchiida; Troglotremata; Troglotrematidae; Paragonimus.
OX NCBI_TaxID=34504 {ECO:0000313|EMBL:KAA3671445.1, ECO:0000313|Proteomes:UP000324629};
RN [1] {ECO:0000313|EMBL:KAA3671445.1, ECO:0000313|Proteomes:UP000324629}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IND2009 {ECO:0000313|EMBL:KAA3671445.1,
RC ECO:0000313|Proteomes:UP000324629};
RX PubMed=30520948;
RA Oey H., Zakrzewski M., Narain K., Devi K.R., Agatsuma T., Nawaratna S.,
RA Gobert G.N., Jones M.K., Ragan M.A., McManus D.P., Krause L.;
RT "Whole-genome sequence of the oriental lung fluke Paragonimus westermani.";
RL Gigascience 8:0-5232231(2019).
CC -!- FUNCTION: Plays a role in pre-mRNA splicing as core component of
CC precatalytic, catalytic and postcatalytic spliceosomal complexes, both
CC of the predominant U2-type spliceosome and the minor U12-type
CC spliceosome. Functions as a scaffold that mediates the ordered assembly
CC of spliceosomal proteins and snRNAs. Required for the assembly of the
CC U4/U6-U5 tri-snRNP complex, a building block of the spliceosome.
CC Functions as a scaffold that positions spliceosomal U2, U5 and U6
CC snRNAs at splice sites on pre-mRNA substrates, so that splicing can
CC occur. Interacts with both the 5' and the 3' splice site.
CC {ECO:0000256|ARBA:ARBA00055739}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAA3671445.1}.
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DR EMBL; QNGE01006444; KAA3671445.1; -; Genomic_DNA.
DR Proteomes; UP000324629; Unassembled WGS sequence.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IEA:TreeGrafter.
DR GO; GO:0005682; C:U5 snRNP; IEA:TreeGrafter.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IEA:TreeGrafter.
DR GO; GO:0030619; F:U1 snRNA binding; IEA:TreeGrafter.
DR GO; GO:0030620; F:U2 snRNA binding; IEA:TreeGrafter.
DR GO; GO:0030623; F:U5 snRNA binding; IEA:InterPro.
DR GO; GO:0017070; F:U6 snRNA binding; IEA:InterPro.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IEA:TreeGrafter.
DR CDD; cd08056; MPN_PRP8; 1.
DR CDD; cd13838; RNase_H_like_Prp8_IV; 1.
DR FunFam; 1.20.80.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.30.420.230:FF:000003; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.40.140.10:FF:000002; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.90.1570.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR Gene3D; 1.20.80.40; -; 1.
DR Gene3D; 3.30.420.230; -; 1.
DR Gene3D; 3.90.1570.40; -; 1.
DR Gene3D; 3.40.140.10; Cytidine Deaminase, domain 2; 1.
DR Gene3D; 3.30.43.40; Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain; 1.
DR InterPro; IPR000555; JAMM/MPN+_dom.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR012592; PROCN.
DR InterPro; IPR012984; PROCT.
DR InterPro; IPR027652; PRP8.
DR InterPro; IPR021983; PRP8_domainIV.
DR InterPro; IPR043173; Prp8_domainIV_fingers.
DR InterPro; IPR043172; Prp8_domainIV_palm.
DR InterPro; IPR019581; Prp8_U5-snRNA-bd.
DR InterPro; IPR042516; Prp8_U5-snRNA-bd_sf.
DR InterPro; IPR019580; Prp8_U6-snRNA-bd.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR019582; RRM_spliceosomal_PrP8.
DR PANTHER; PTHR11140; PRE-MRNA SPLICING FACTOR PRP8; 1.
DR PANTHER; PTHR11140:SF0; PRE-MRNA-PROCESSING-SPLICING FACTOR 8; 1.
DR Pfam; PF01398; JAB; 1.
DR Pfam; PF08083; PROCN; 1.
DR Pfam; PF08084; PROCT; 1.
DR Pfam; PF12134; PRP8_domainIV; 1.
DR Pfam; PF10598; RRM_4; 1.
DR Pfam; PF10597; U5_2-snRNA_bdg; 1.
DR Pfam; PF10596; U6-snRNA_bdg; 1.
DR SMART; SM00232; JAB_MPN; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 2.
DR PROSITE; PS50249; MPN; 1.
PE 4: Predicted;
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000324629};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Spliceosome {ECO:0000256|ARBA:ARBA00022728}.
FT DOMAIN 1404..1532
FT /note="MPN"
FT /evidence="ECO:0000259|PROSITE:PS50249"
SQ SEQUENCE 1633 AA; 189911 MW; 18FCE3E7E40BF1D4 CRC64;
MYSLALSIKV PNMPIPIENM ILRYVKAKAD WWTNTAHYNR ERIRRGATVD KTVCKKNLGR
LTRLYLKAEQ ERQHNYLKDG PYVTAEEAVA IYTTTVHWLE SRRFSPIPFP PLSYKHDTKL
LILALERLRE SYSVKNRLNQ SQREELGLIE QAYDNPHEAL SRIKRHLLTQ RAFKEVGIEF
MDLYSNLVPV YDVEPLEKIT DAYLDQYLWY EADKRRLFPP WIKPADLEPP PLLVYKWCQG
INNLKDVWET GEGECNVLLE TKFEKVYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN
YKDMNHTNSY GIIRGLQFAS FITQYFGLVL DLLVLGLERA AEMCGPPQMP NDFLQFQDTA
TEVAHPIRLY CRYVDRFWMF FRFTAEEARD LIQRYLTEHP DPNNENIVGY NNKKCWPRDS
RMRLMKHDVN LGRAVFWDIK NRLPRSITTT DWDSSFVSVY SRDNPNLLFA MSGFECRILP
ACRAPSQAAD ANLPSGVWHL QNERGFRISS NFMLAFNVFK VTKERTAQCF LRVDDESMQR
FHNRVRQILM ASGSTTFTKI VNKWNTALIG LMTYFREAAV STVPLLDLLV KCENKIQTRI
KIGLNSKMPA RFPPVVFYTP KELGGLGMLS MGHVLIPQSD LRWSKQTDVG ITHFRSGMSH
EEDQVIPNLF SYILTWETEF RDSQSVWAEY ALKRQEANAQ NRRLTLEDLE DSWDRGIPRI
NTLFQKDRHT LAYDKGWRVR TEFKQYQVLK QNPFWWTHQR HDGKLWNLNN YRTDMIQALG
GVEGILEHTL FKGTYFPTWE GLFWEKASGF EESMKYKKLT NAQRSGLNQI PNRRFTLWWS
PTINRANVYV GFQVQLDLTG IFMHGKIPTL KISLIQIFRA HLWQKIHESV VMDICQVFDQ
ELDALEIETV QKETIHPRKS YKMNSSCADI LLFASYKWPV SRPSLLADSK DLMDGTTTQK
FWIDIQLRWG DYDSHDVERY ARAKFLDYTT DNMSIYPSPT GVMIAFDLAY NLHSAYGNWF
PGCKPLIQQA MVKIMKANPA LYVLRERIRK ALQLYSSEPT EPYLSSQNYN ELFSNQIIWF
VDDTNVYRVT IHKTFEGNLT TKPINGAIFI FNPRTGQLFL KIIHTSVWAG QKRLGQLAKW
KTAEEVAALI RSLPVEEQPK QIIVTRKGML DPLEVHLLDF PNIVIKGSEL QLPFQACLKV
EKFGDLILKA SEPQMVLFNL YDDWLKSISS YTAFSRLILI LRALHVNNDK AKIVLKPDKT
TVTEPHHIWP SLSPDDWIKV EYQLKDLILA DYGKKNNVNV ASLTQSEIRD IILGMEISAP
SQQRQQIAEI EKQAKEQSQL TATTTRTVNK HGDEIISTTT SNYETLHFSS KTEWRVRAIS
ATNLYLRTNN IYVSSDDIKE NGYTYILPKN ILKKFITISD LRTQIAGYLY GVSPPDNSQI
KEIRCIVMPP QWGTHQTVHL PNGLPQDEYL REMEPLGWIH TQPNELPQLS PQDITTHAKI
FSEQDGEKTI VITCSFTPGS VSLCAHKLTP GGYEWGRQNT DKGNNPKGYL PSHYERVQML
LSDRFLGFFM VPPQSSWNYN FMGVRHDPNM KYELQPLKPK KFYHRIHRPS HFLNFTSIEE
TELTSVDRDN PFA
//
