ID A0A5J5F870_9PEZI Unreviewed; 374 AA.
AC A0A5J5F870;
DT 11-DEC-2019, integrated into UniProtKB/TrEMBL.
DT 11-DEC-2019, sequence version 1.
DT 08-OCT-2025, entry version 14.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Name=NCS2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Synonyms=CTU2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN ORFNames=FN846DRAFT_930205 {ECO:0000313|EMBL:KAA8913239.1};
OS Sphaerosporella brunnea.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC Pezizales; Pyronemataceae; Sphaerosporella.
OX NCBI_TaxID=1250544 {ECO:0000313|EMBL:KAA8913239.1, ECO:0000313|Proteomes:UP000326924};
RN [1] {ECO:0000313|EMBL:KAA8913239.1, ECO:0000313|Proteomes:UP000326924}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sb_GMNB300 {ECO:0000313|EMBL:KAA8913239.1,
RC ECO:0000313|Proteomes:UP000326924};
RG DOE Joint Genome Institute;
RA Benucci G.M., Marozzi G., Antonielli L., Sanchez S., Marco P., Wang X.,
RA Falini L.B., Barry K., Haridas S., Lipzen A., Labutti K., Grigoriev I.V.,
RA Murat C., Martin F., Albertini E., Donnini D., Bonito G.;
RT "Draft genome of the ectomycorrhizal ascomycete Sphaerosporella brunnea.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with NCS6 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC Prior mcm(5) tRNA modification by the elongator complex is required for
CC 2-thiolation. May also be involved in protein urmylation.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAA8913239.1}.
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DR EMBL; VXIS01000016; KAA8913239.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5J5F870; -.
DR FunCoup; A0A5J5F870; 180.
DR InParanoid; A0A5J5F870; -.
DR OrthoDB; 25129at2759; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000326924; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000326924};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 374 AA; 40985 MW; 6F5619B5D3A229B4 CRC64;
MPAAAEAQQC KRCHAQTSAH ILRSTPHCVG CYDHYIRSKC VKRMESYKIK SENPPITLLL
ALSFGASSTT LLHLLDSQIR AQKHRIRYRL KILHVDERIL DPSLPASESL EEVKRRFPQT
GGYLSARLED VYDFAAEETG LERDGEDGGG KEEKLKTLLQ RLPSPTSRED LAAVLRTRLI
VALARREGCS GILWGDTTTR LADKVLASAA KGRGAQLPFE IGDGPGPFGI TYYRPLRDVF
KKELVTYISE IVTPPLLPLC VGFSSTPKHS AAAATSGKNI TVGELMTQYF ESMEAQYPST
VSNVVRMGDR LKLPGRGEEE DSCAMCGLLM DKGGNGLTLG AQEVVEGGER RKVCYGCMRA
THGASAELSW PVLS
//
