ID A0A5M3VNK2_9ACTN Unreviewed; 560 AA.
AC A0A5M3VNK2;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 28-JAN-2026, entry version 22.
DE RecName: Full=assimilatory sulfite reductase (ferredoxin) {ECO:0000256|ARBA:ARBA00012353};
DE EC=1.8.7.1 {ECO:0000256|ARBA:ARBA00012353};
GN ORFNames=Acor_04260 {ECO:0000313|EMBL:GER98364.1};
OS Acrocarpospora corrugata.
OC Bacteria; Bacillati; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Streptosporangiaceae; Acrocarpospora.
OX NCBI_TaxID=35763 {ECO:0000313|EMBL:GER98364.1, ECO:0000313|Proteomes:UP000334990};
RN [1] {ECO:0000313|EMBL:GER98364.1, ECO:0000313|Proteomes:UP000334990}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 13972 {ECO:0000313|EMBL:GER98364.1,
RC ECO:0000313|Proteomes:UP000334990};
RA Ichikawa N., Kimura A., Kitahashi Y., Komaki H., Oguchi A.;
RT "Whole genome shotgun sequence of Acrocarpospora corrugata NBRC 13972.";
RL Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the reduction of sulfite to sulfide, a step in the
CC biosynthesis of sulfur-containing amino acids and cofactors.
CC {ECO:0000256|ARBA:ARBA00003247}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydrogen sulfide + 6 oxidized [2Fe-2S]-[ferredoxin] + 3 H2O =
CC sulfite + 6 reduced [2Fe-2S]-[ferredoxin] + 7 H(+);
CC Xref=Rhea:RHEA:23132, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17359,
CC ChEBI:CHEBI:29919, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738; EC=1.8.7.1;
CC Evidence={ECO:0000256|ARBA:ARBA00049518};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- COFACTOR:
CC Name=siroheme; Xref=ChEBI:CHEBI:60052;
CC Evidence={ECO:0000256|ARBA:ARBA00001929};
CC -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC family. {ECO:0000256|ARBA:ARBA00010429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GER98364.1}.
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DR EMBL; BLAD01000036; GER98364.1; -; Genomic_DNA.
DR RefSeq; WP_155334810.1; NZ_BAAABN010000078.1.
DR AlphaFoldDB; A0A5M3VNK2; -.
DR OrthoDB; 3189055at2; -.
DR Proteomes; UP000334990; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0050311; F:sulfite reductase (ferredoxin) activity; IEA:UniProtKB-EC.
DR FunFam; 3.30.413.10:FF:000009; Sulfite reductase [ferredoxin]; 1.
DR FunFam; 3.30.413.10:FF:000013; Sulfite reductase [ferredoxin]; 1.
DR Gene3D; 3.90.480.20; -; 1.
DR Gene3D; 3.30.413.10; Sulfite Reductase Hemoprotein, domain 1; 2.
DR InterPro; IPR051329; NIR_SIR_4Fe-4S.
DR InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR InterPro; IPR036136; Nit/Sulf_reduc_fer-like_dom_sf.
DR InterPro; IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR InterPro; IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS.
DR PANTHER; PTHR32439; FERREDOXIN--NITRITE REDUCTASE, CHLOROPLASTIC; 1.
DR PANTHER; PTHR32439:SF0; FERREDOXIN--NITRITE REDUCTASE, CHLOROPLASTIC; 1.
DR Pfam; PF01077; NIR_SIR; 2.
DR Pfam; PF03460; NIR_SIR_ferr; 2.
DR PRINTS; PR00397; SIROHAEM.
DR SUPFAM; SSF56014; Nitrite and sulphite reductase 4Fe-4S domain-like; 2.
DR SUPFAM; SSF55124; Nitrite/Sulfite reductase N-terminal domain-like; 2.
DR PROSITE; PS00365; NIR_SIR; 2.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Heme {ECO:0000256|ARBA:ARBA00022617}; Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000334990};
KW Thioether bond {ECO:0000256|ARBA:ARBA00022784}.
FT DOMAIN 96..159
FT /note="Nitrite/Sulfite reductase ferredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF03460"
FT DOMAIN 167..319
FT /note="Nitrite/sulphite reductase 4Fe-4S"
FT /evidence="ECO:0000259|Pfam:PF01077"
FT DOMAIN 344..410
FT /note="Nitrite/Sulfite reductase ferredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF03460"
FT DOMAIN 420..555
FT /note="Nitrite/sulphite reductase 4Fe-4S"
FT /evidence="ECO:0000259|Pfam:PF01077"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 560 AA; 62354 MW; 556E889C3751B2DF CRC64;
MSTPARPGNR HHKRPRGEGQ WALGYREPLN KNEENKKNDD GLNVRQRIID IYSKRGFDSI
DPADLRGRMR WYGLYTQRKP GIDGGKTAIL EPEELDDRYF MLRIRIDGGQ LSLAQLRVIA
DISNEFGRGT ADITDRQNVQ LHWVEVESVP EIWQRLEAVG LSTTEACGDT PRVILGCPLA
GVDVAEVIDG TEQVAEIYER FIGDKAFSNL PRKFKSAVSG CPAHCTVHEI NDIAFVGVVN
EAGEPGFDAW VGGGLSTNPM IGKRLGVFVR PDQVADVYGG VVGIFRDYGY RRLRHRARIK
FLMNDWGPER FREVLQKDYL GYELPDGPAP EEPRGGRRDH VGVHPQRDGN FYVGFAPKVG
RVDGDTLHRV ADIAERHGSD RVRTTVEQKM VILDIAPDLV DSVVAELEAA DLQVNPSTFR
RQTMACTGIE YCKLAIVETK ATATTLIHEL ENRLPDFKDP LTINVNGCPN SCARIQVADI
GLKGQLVVNE HGEQVEGFQI HLGGSLGPTP GFGRKVRGLK ATAAELPDYV ERVVGNFDKQ
KNDGERFADW VQRADESDLK
//
