ID A0A5N4CMT6_CAMDR Unreviewed; 531 AA.
AC A0A5N4CMT6;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 10-JUN-2026, entry version 13.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Name=CTU2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Synonyms=NCS2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN ORFNames=Cadr_000024977 {ECO:0000313|EMBL:KAB1260255.1};
OS Camelus dromedarius (Dromedary) (Arabian camel).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Tylopoda; Camelidae; Camelus.
OX NCBI_TaxID=9838 {ECO:0000313|EMBL:KAB1260255.1, ECO:0000313|Proteomes:UP000299084};
RN [1] {ECO:0000313|EMBL:KAB1260255.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Drom800 {ECO:0000313|EMBL:KAB1260255.1};
RC TISSUE=Blood {ECO:0000313|EMBL:KAB1260255.1};
RA Fitak R., Mohandesan E., Burger P.A., Jukka C.;
RL Submitted (DEC-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAB1260255.1, ECO:0000313|Proteomes:UP000299084}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Drom800 {ECO:0000313|EMBL:KAB1260255.1};
RC TISSUE=Blood {ECO:0000313|EMBL:KAB1260255.1};
RX PubMed=30972949; DOI=.1111/1755-0998.13020;
RA Elbers J.P., Rogers M.F., Perelman P.L., Proskuryakova A.A.,
RA Serdyukova N.A., Johnson W.E., Horin P., Corander J., Murphy D.,
RA Burger P.A.;
RT "Improving Illumina assemblies with Hi-C and long reads: an example with
RT the North African dromedary.";
RL Mol. Ecol. Resour. 19:1015-1026(2019).
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBUNIT: Component of a complex at least composed of URM1, CTU2/NCS2
CC and CTU1/ATPBD3. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAB1260255.1}.
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DR EMBL; JWIN03000021; KAB1260255.1; -; Genomic_DNA.
DR EMBL; JWIN03000021; KAB1260256.1; -; Genomic_DNA.
DR STRING; 9838.ENSCDRP00005030026; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000299084; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000299084};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..10
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 531 AA; 57237 MW; 168F600ECFC66A07 CRC64;
MCEVGEDYRE SAPGGPPLTR PGREQKCVKC KEGPPVVVIR AGDAFCRDCF KVFYVHKFRA
MLGKNRLIFP GEKVLLAWSG GPSSSSMVWQ VLEGLSRDSA KRLRFVPGVI YVDGAVVVAL
TARVRLFCKV VIPHFPEGAA CGRSPEDRAR TLVEVEQVLQ RVGFPSHVIA LEEVFGLPPS
VLRCFAQEPE SVGTEGAYKA AVDSFLQQQC VLGAAGEEPW SQPRPQGPQS LAGPPTAVQT
EALSGLFDSV KTLTAKEELL QTLRTHLILH VARTHGYSKV MAGDSCTRLA VKLMTSLALG
RGAFLAWDTG FSDERHGDVV VVRPMREHTL KEVAFYNRLF AVPSVFTPAV DTKAPEKASI
HRLMEAFLLR LQAQFPSTVS TVYRTSEKLV KAPRDGCAAS PPGPRCLLCL CTLDVDSADS
ATAFGAQTSS HLCQMQAPGP QAEAGTPTTL CCCPGLGGAQ GCCAREDPRA RVVGQLCYGC
RVNARDLPSL DSLPPYILAE AQLRSQRAGG EQEIQAYLLG DRDDEALPGG S
//
