ID A0A5N5U3Z1_9EURY Unreviewed; 1582 AA.
AC A0A5N5U3Z1;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 18-JUN-2025, entry version 28.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=DM867_09820 {ECO:0000313|EMBL:KAB7513273.1};
OS Halosegnis rubeus.
OC Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
OC Halobacteria; Halobacteriales; Haloarculaceae; Halosegnis.
OX NCBI_TaxID=2212850 {ECO:0000313|EMBL:KAB7513273.1, ECO:0000313|Proteomes:UP000326865};
RN [1] {ECO:0000313|EMBL:KAB7513273.1, ECO:0000313|Proteomes:UP000326865}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F18-79 {ECO:0000313|EMBL:KAB7513273.1,
RC ECO:0000313|Proteomes:UP000326865};
RA Duran-Viseras A., Andrei A.-S., Vera-Gargallo B., Ghai R.,
RA Sanchez-Porro C., Ventosa A.;
RT "Unraveling microbial dark matter from salterns through culturing: the case
RT of the genus Halosegnis.";
RL Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00048988};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
CC {ECO:0000256|ARBA:ARBA00006637}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAB7513273.1}.
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DR EMBL; QKKZ01000004; KAB7513273.1; -; Genomic_DNA.
DR Proteomes; UP000326865; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0140097; F:catalytic activity, acting on DNA; IEA:UniProtKB-ARBA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00081; Hint; 2.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 3.
DR Gene3D; 3.10.28.10; Homing endonucleases; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR050615; ATP-dep_DNA_Helicase.
DR InterPro; IPR032438; ERCC3_RAD25_C.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C-like.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc_central.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 2.
DR PANTHER; PTHR11274:SF0; GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB; 1.
DR PANTHER; PTHR11274; RAD25/XP-B DNA REPAIR HELICASE; 1.
DR Pfam; PF16203; ERCC3_RAD25_C; 1.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 2.
DR Pfam; PF04851; ResIII; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00305; HintC; 2.
DR SMART; SM00306; HintN; 2.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 2.
DR SUPFAM; SSF55608; Homing endonucleases; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 2.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 2.
DR PROSITE; PS50817; INTEIN_N_TER; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022806};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Reference proteome {ECO:0000313|Proteomes:UP000326865}.
FT DOMAIN 269..374
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 498..630
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 1083..1220
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 1433..1582
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1555..1582
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 519..546
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1582 AA; 175706 MW; C662E25E4C8E849B CRC64;
MTDDADESVE PAIRRVDADL TLDTFYDALQ SVGRPLATAS ELARELDVSQ SVALDALESL
ARTGRIDSAD VETDPRVWFP SDYKETTDRE RVVVFPGRRQ VVVDGPSQFT RAQLSQFAHL
EDANRESGYI YEVREEDVWS APYDEFSGLQ RTMRQALGER SDALEEWVRS QWERARKFRL
YSHEDGYTVL EAKSADLMGN IAEQELEEGD LRALISDTEA WIADDAVAGV KRTLYEAGYP
VLDTRHLDTG DDLPIELEVD LRDYQNEWVN RFTKKRSGIL VGPPGSGKTV AAMGVMSAIE
GETLVLVPGR ELAAQWKETI VEHTSLSPDV IGEYHGGKKE IRPVTIATYQ TAGMDRHRHL
FDDRKWGLIV YDECLTGETT VETPARKTTF SQLDERYEFE QGWNDGIDEQ VRTHDPGGGS
EWTEVTGVYK NEAPVQRIET NTGHVLRATP SHTHLVFDPE SCEIQEQRGV SEGEFLVMPL
PEPDETARES DGAASAELLG WFIGDGHMNE YDDVKFSFAR RADEQIRILE ILCERLETDY
STFENERGDR TLWAPKLREP LGWSGQHGDK TTTVSVPDEG FGWSDSDIGA LLRGLFDAEG
SVDSKGRIQF NTTSEALAND VGLLLRRLGI LSRSFSIERE ADQHNTVHRI TIPTHYNDQF
TTYVGFRLEH KSSRVEAGHS PATGIPAGQY LETVKIDTGL TNEQLGEMAG IAKGTVGDTI
RGAYQLGQPR LDTLAEGLNA AGTKSFEDAI AAKEFAGISY RVLGEALGVA TSTAEQRLKR
GERDAVQAVE ELLDRRQSAM LRHSKRLSSL SELCLLEVAD VQDDGVETVY DFETASHTFI
ANGILTHNCH HIPSPIHRRS ATLQAKHRLG LSVDGETMVP IRNGGELSMR PIAEFAEEHL
AVGPGMERLS GMETLGVTED GAVEWTPIQA VMRHRHDGQM YRVRGRNGRE VTVTGDHSLI
VFDGETMEVT SKVAEDLSET DYLLQPEAVP APGATDQTVD VMELLEEGYV LIDDDAPESV
FDPLYERGIG DNKSRYNWKS RRSIPLSIAR EIDLNRECIK GVYVHGRHSY IPPEVSTEAF
ARLLGLFVAD GALDDGRVEF YATDSEEKSE VVAFEQVIRG VCPEVDISYV ANGENCTTLR
VTGPLVRVLR SLGLSNGARD KSVPSVVLSN PTAYEPFIEG IVLGDGHRAK RERGKEMVTI
STSSKQLAAG LNLLLAARGY VGGEYHRNPE VGVREGDHDT VENYLVRFNP NNDGQTGRLS
LTPFTEPLQR AYDEAERYGP RRADGGTFES GLAERMRLND DELSAVTQRG DVDAEWLTET
DVAMLDVESV VEVDSEDEYV YDLSTGTENF LGDHLFCHNS ATPIREDDKQ QEIYTLIGPP
IGTNWAALFD AGYVAEPEVE IRLVPWADDD ARREYDAAVG HDKRKAAAEN PAKLPEIEAI
LREHRGEKAL VFVEYIEQGE QIAEALSVPF VSGEMRHARR RKLFDEFRHG ARDVLVVSRV
ADEGIDLPGA EIAIAASGLG GSRRQGAQRA GRTMRPVGKA RMYVLATRAS KEEEFARSRT
RHLQGKGIRV QERGAETADE SE
//
