ID A0A5N6PKP4_9ASTR Unreviewed; 587 AA.
AC A0A5N6PKP4;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 18-JUN-2025, entry version 19.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN ORFNames=E3N88_08911 {ECO:0000313|EMBL:KAD6454205.1};
OS Mikania micrantha.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC Heliantheae alliance; Eupatorieae; Mikania.
OX NCBI_TaxID=192012 {ECO:0000313|EMBL:KAD6454205.1, ECO:0000313|Proteomes:UP000326396};
RN [1] {ECO:0000313|EMBL:KAD6454205.1, ECO:0000313|Proteomes:UP000326396}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NLD-2019 {ECO:0000313|EMBL:KAD6454205.1};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAD6454205.1};
RA Liu B.;
RT "Mikania micrantha, genome provides insights into the molecular mechanism
RT of rapid growth.";
RL Submitted (MAY-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAD6454205.1}.
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DR EMBL; SZYD01000004; KAD6454205.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A5N6PKP4; -.
DR OrthoDB; 10009520at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000326396; Miscellaneous, Linkage group lg12.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd20346; BRcat_RBR_ANKIB1; 1.
DR CDD; cd22586; Rcat_RBR_ARI1-like; 1.
DR CDD; cd16773; RING-HC_RBR_TRIAD1; 1.
DR FunFam; 1.20.120.1750:FF:000013; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000019; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR045840; Ariadne.
DR InterPro; IPR048962; ARIH1-like_UBL.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR054694; Parkin-like_IBR.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF19422; Ariadne; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF22605; IBR_2; 1.
DR Pfam; PF21235; UBA_ARI1; 1.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000326396};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 119..333
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 123..171
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
SQ SEQUENCE 587 AA; 67876 MW; 44A4DE279D8BFB85 CRC64;
MEFVDDCLSS GEEDYYCSDR ESLEDALEND DSDSHFAPPK GSSSKVITQE SLLAAQREDL
CRVMELLPLK EHHARTLLIH YRWDVEKIFA VLVEKGKYCL FTEAGVPMSH DSNSSMSTST
TTCDICMEDL LSSEMTKMDC GHCFCNSCWT EHFIVKINEG QSRRIRCMSH KCFAICDEAT
IRTLVNKRHP DLAKKFDRFL LESYIEDNKM VKWCPSTPHC GNAIRVEDDE CCEVECSCGN
QFCFSCLCEA HSPCSCTMWE LWVKKCRDES ETVNWITVYT KPCPKCHKPV EKNGGCNLVG
CICGQAFCWL CGGATGREHT WSTITGHSCG RYKEDQEKKS EHAKRDLYRY MHYYNRYKAH
KDSFKQESRL KETIRDKVAV LEGKNSELRD FSWVTNGLYR LFRSRRALSY SYPFAFYMFG
AELFHDEMTK EDLEIKQNLF EDQQQQLETN VEKLSKFIEE PFEGYSEDNI MKMRMQVINL
SIITDTLCKK MYEYIEMDLL GSLQYGIHNI APYHSKGVEK ATELSISWAA KSCNDDMCQN
LEDSTNCIER LTRQSRQQIV TLFSQPSTLV ALEDEDDADS PGEDRRI
//
