ID A0A5Q2FE95_9ACTN Unreviewed; 585 AA.
AC A0A5Q2FE95;
DT 26-FEB-2020, integrated into UniProtKB/TrEMBL.
DT 26-FEB-2020, sequence version 1.
DT 28-JAN-2026, entry version 27.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:QGF24701.1};
GN ORFNames=Rai3103_14845 {ECO:0000313|EMBL:QGF24701.1};
OS Raineyella fluvialis.
OC Bacteria; Bacillati; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Propionibacteriaceae; Raineyella.
OX NCBI_TaxID=2662261 {ECO:0000313|EMBL:QGF24701.1, ECO:0000313|Proteomes:UP000386847};
RN [1] {ECO:0000313|EMBL:QGF24701.1, ECO:0000313|Proteomes:UP000386847}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBA3103 {ECO:0000313|EMBL:QGF24701.1,
RC ECO:0000313|Proteomes:UP000386847};
RA Roh S.W.;
RT "Genomic analysis of Raineyella sp. CBA3103.";
RL Submitted (OCT-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; CP045725; QGF24701.1; -; Genomic_DNA.
DR RefSeq; WP_153573230.1; NZ_CP045725.1.
DR AlphaFoldDB; A0A5Q2FE95; -.
DR KEGG; rain:Rai3103_14845; -.
DR Proteomes; UP000386847; Chromosome.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0035368; F:selenocysteine insertion sequence binding; IEA:TreeGrafter.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0000049; F:tRNA binding; IEA:TreeGrafter.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR GO; GO:0016259; P:selenocysteine metabolic process; IEA:TreeGrafter.
DR CDD; cd04171; SelB; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR050543; eIF2G.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR NCBIfam; TIGR00475; selB; 1.
DR PANTHER; PTHR42854; EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER; 1.
DR PANTHER; PTHR42854:SF3; EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Elongation factor {ECO:0000313|EMBL:QGF24701.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000386847}.
FT DOMAIN 1..167
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 585 AA; 62866 MW; E81EBFD94051586B CRC64;
MRVIATAGHV DHGKSTLVRA LTGIEPDRWA EERARGLTID LGFAWTRLAS GEEVAFVDVP
GHERFIANML AGLGPVPAVM LVVAADEGWR AQSQEHLDAI TALGISHGLL VITRKDLADP
APALGQARER LRSTPLAGCE AVSVSAMTGD GVEELRGALT RLIASLPAPA PSSRVRLWID
RSFTIRGAGT VVTGTLESGT LRVGDRVQLP GRTVGIRALE SLGQDHPQVS APARVAVNLR
DVAADEVVRG DTLLTGAWHH SEVVDVALNP VPDRLPGHLM AHVGTTSRAV HVRLLGTRFA
RISWRAALPL QAGDRLVLRD PGQQRVLAGA VVLDADPPDL DRRGAARQRA AELEDASHHL
DPAREVHRRG WMTRHDLQLL GGSPDEVLAG VHRSGHYLID GEVWEGWAEA LRQSVAAYAA
TNPLRPRMPM AAAAGVLGLP DRALLAPLAE DAGLELCEGH VARPGTRPTL GPAEPGLVEI
ERRLARQPFL APERQELTEL GLGPRQIAAA VSLGRLLDLG DQIVLAPTAP ALAMRRLASL
PQPFTTSEAR QALDTTRRVV IPLLEHLDHR GWTRRLDAGH REVVR
//
