ID   A0A671MEA1_9TELE        Unreviewed;       562 AA.
AC   A0A671MEA1;
DT   17-JUN-2020, integrated into UniProtKB/TrEMBL.
DT   17-JUN-2020, sequence version 1.
DT   18-JUN-2025, entry version 21.
DE   RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE            EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
OS   Sinocyclocheilus anshuiensis.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Cyprininae; Sinocyclocheilus.
OX   NCBI_TaxID=1608454 {ECO:0000313|Ensembl:ENSSANP00000030757.1, ECO:0000313|Proteomes:UP000472260};
RN   [1] {ECO:0000313|Ensembl:ENSSANP00000030757.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (MAR-2025) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC         EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000256|ARBA:ARBA00004906}.
CC   -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC       {ECO:0000256|ARBA:ARBA00005884}.
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DR   AlphaFoldDB; A0A671MEA1; -.
DR   Ensembl; ENSSANT00000032734.1; ENSSANP00000030757.1; ENSSANG00000015705.1.
DR   Proteomes; UP000472260; Unassembled WGS sequence.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR   CDD; cd20343; BRcat_RBR_HHARI-like; 1.
DR   CDD; cd20356; Rcat_RBR_HHARI-like; 1.
DR   CDD; cd16626; RING-HC_RBR_HHARI; 1.
DR   FunFam; 1.20.120.1750:FF:000002; RBR-type E3 ubiquitin transferase; 1.
DR   FunFam; 3.30.40.10:FF:000019; RBR-type E3 ubiquitin transferase; 1.
DR   Gene3D; 1.20.120.1750; -; 1.
DR   Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR   InterPro; IPR045840; Ariadne.
DR   InterPro; IPR048962; ARIH1-like_UBL.
DR   InterPro; IPR031127; E3_UB_ligase_RBR.
DR   InterPro; IPR002867; IBR_dom.
DR   InterPro; IPR044066; TRIAD_supradom.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR   Pfam; PF19422; Ariadne; 1.
DR   Pfam; PF01485; IBR; 1.
DR   Pfam; PF22191; IBR_1; 1.
DR   Pfam; PF21235; UBA_ARI1; 1.
DR   SMART; SM00647; IBR; 2.
DR   SUPFAM; SSF57850; RING/U-box; 3.
DR   PROSITE; PS51873; TRIAD; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   3: Inferred from homology;
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000472260};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833};
KW   Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW   ProRule:PRU00175}.
FT   DOMAIN          187..398
FT                   /note="RING-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51873"
FT   DOMAIN          191..240
FT                   /note="RING-type"
FT                   /evidence="ECO:0000259|PROSITE:PS50089"
FT   REGION          82..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   562 AA;  65578 MW;  43217A1B37E824FD CRC64;
     ITYFETAKSS SSKFCISTMF SLTPSRSLGF YRNPVMDSDE GYNYEFDDEE EECSEDSGEE
     ETADDTLELG EVELLDAVVA GGERDDCGET GGSGLGPGQD EEDYRFEVLT AEQILQHMVE
     CIREVNEVIQ NPATITRILL SHFNWDKEKL MERYFDGNLD KLFSECHVIN PSKKSRTRLM
     NTRSSAQDMP CQICYLNYPN SYFTGLECGH KFCMQCWGDY LTTKIIEEGM GQTISCPAHS
     CDILVDDSTV MRLITDLKVK LKYQHLITNS FVECNRLLKW CPAPDCHHVV KVQYPDAKPV
     RCKCGRQFCF NCGENWHDPV KCKWLRKWIK KCDDDSETSN WIAANTKECP KCHVTIEKDG
     GCNHMVCRNQ NCKAEFCWVC LGPWEPHGSA WYNCNRYNED DAKAARDAQE RSRAALQRYL
     FYCNRYMNHM QSLRFEHKLY AQVKQKMEEM QQHNMSWIEV QFLKKAVDVL CQCRSTLMFT
     YVFAFYLKKN NQSIIFENNQ ADLENATEVL SGYLERDISQ DSLQDIKQKV QDKYRYCESR
     RRVLLQHVHE GYDKDLWEYI ED
//