ID A0A6A4WG12_AMPAM Unreviewed; 486 AA.
AC A0A6A4WG12;
DT 17-JUN-2020, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 1.
DT 02-APR-2025, entry version 17.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN Name=RNF14_1 {ECO:0000313|EMBL:KAF0304169.1};
GN ORFNames=FJT64_023976 {ECO:0000313|EMBL:KAF0304169.1};
OS Amphibalanus amphitrite (Striped barnacle) (Balanus amphitrite).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Multicrustacea;
OC Cirripedia; Thoracica; Thoracicalcarea; Balanomorpha; Balanoidea;
OC Balanidae; Amphibalaninae; Amphibalanus.
OX NCBI_TaxID=1232801 {ECO:0000313|EMBL:KAF0304169.1, ECO:0000313|Proteomes:UP000440578};
RN [1] {ECO:0000313|EMBL:KAF0304169.1, ECO:0000313|Proteomes:UP000440578}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SNU_AA5 {ECO:0000313|EMBL:KAF0304169.1};
RC TISSUE=Soma without cirri and trophi {ECO:0000313|EMBL:KAF0304169.1};
RA Kim W.;
RT "Draft genome assembly of a fouling barnacle, Amphibalanus amphitrite
RT (Darwin, 1854): The first reference genome for Thecostraca.";
RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the RBR family. RNF14 subfamily.
CC {ECO:0000256|ARBA:ARBA00044508}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF0304169.1}.
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DR EMBL; VIIS01000868; KAF0304169.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A6A4WG12; -.
DR EnsemblMetazoa; XM_043386521.1; XP_043242456.1; LOC122392039.
DR EnsemblMetazoa; XM_043386523.1; XP_043242458.1; LOC122392039.
DR EnsemblMetazoa; XM_043386524.1; XP_043242459.1; LOC122392039.
DR OrthoDB; 69641at2759; -.
DR Proteomes; UP000440578; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR GO; GO:0060255; P:regulation of macromolecule metabolic process; IEA:UniProtKB-ARBA.
DR CDD; cd20341; BRcat_RBR_RNF14; 1.
DR CDD; cd20354; Rcat_RBR_RNF14; 1.
DR CDD; cd16628; RING-HC_RBR_RNF14; 1.
DR CDD; cd23820; RWD_RNF14; 1.
DR FunFam; 3.30.40.10:FF:000137; RanBP-type and C3HC4-type zinc finger-containing protein 1; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 2.20.25.20; -; 1.
DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR047548; Rcat_RBR_RNF14.
DR InterPro; IPR031128; RNF14_RING-HC_Zfn.
DR InterPro; IPR006575; RWD_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF22191; IBR_1; 1.
DR Pfam; PF05773; RWD; 1.
DR SMART; SM00647; IBR; 2.
DR SMART; SM00184; RING; 1.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR SUPFAM; SSF54495; UBC-like; 1.
DR PROSITE; PS50908; RWD; 1.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000440578};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 9..129
FT /note="RWD"
FT /evidence="ECO:0000259|PROSITE:PS50908"
FT DOMAIN 211..452
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 215..264
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
SQ SEQUENCE 486 AA; 54662 MW; FB36AFD3C9A158B1 CRC64;
MEDREQQREE LLVMDSIFGH DGLFTSDDKQ MSGMISVSPE LQRPLLVKPP EPDKEPLSVS
ALPPICIEFT LPEQYPLEAP PTNRVTCSWL TPTQMLKIWR HLVTLWEQGQ GNTVLYEYTE
FLRNDSIAFL GLEHELDVSF FVDRARTAAA AAAAGPPSPA AGSSPPRSLN RRASVEQLRA
RYRLTLPASA SVHRLLETHQ RQAEAAEFGR RRFPCKVCGE ERAGSDCVRL TRCGHVYCRD
CLTAYYTVLI RDGEVQNLRC PEDQCPEQAT PKEVRDLVGD DLFERYDTIL LRTSLSAMTD
VVTCPRAHCQ KPVVLDPGDS LGRCAHCDHT FCAICQNAYH GNNPCAVKSG ERLELVRQYR
VAGAGERADL ERRYGRRQLQ RLVEEVDSAA WMSDHSERCP RCRTPIEKID GCNKMSCTKC
GTLFCWLCNA RLPRENPYTH FNVPGSKCFN QLFAGVVDGA LLDEELGALA FDELGEEEDL
PMGFFL
//
