ID A0A6A5F2L5_PERFL Unreviewed; 569 AA.
AC A0A6A5F2L5;
DT 17-JUN-2020, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 1.
DT 08-OCT-2025, entry version 21.
DE RecName: Full=TNF receptor-associated factor {ECO:0000256|PIRNR:PIRNR015614};
DE EC=2.3.2.27 {ECO:0000256|PIRNR:PIRNR015614};
GN ORFNames=PFLUV_G00065840 {ECO:0000313|EMBL:KAF1388746.1};
OS Perca fluviatilis (European perch).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Percoidei; Percidae; Percinae; Perca.
OX NCBI_TaxID=8168 {ECO:0000313|EMBL:KAF1388746.1, ECO:0000313|Proteomes:UP000465112};
RN [1] {ECO:0000313|EMBL:KAF1388746.1, ECO:0000313|Proteomes:UP000465112}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Blood {ECO:0000313|EMBL:KAF1388746.1};
RA Roques C., Zahm M., Cabau C., Klopp C., Bouchez O., Donnadieu C., Kuhl H.,
RA Gislard M., Guendouz S., Journot L., Haffray P., Bestin A., Morvezen R.,
RA Feron R., Wen M., Jouanno E., Herpin A., Schartl M., Postlethwait J.,
RA Schaerlinger B., Chardard D., Lecocq T., Poncet C., Jaffrelo L.,
RA Lampietro C., Guiguen Y.;
RT "A chromosome-scale genome assembly of the European perch, Perca
RT fluviatilis.";
RL Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|PIRNR:PIRNR015614};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|PIRNR:PIRNR015614}.
CC -!- SIMILARITY: Belongs to the TNF receptor-associated factor family. A
CC subfamily. {ECO:0000256|ARBA:ARBA00006608}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF1388746.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; VHII01000006; KAF1388746.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A6A5F2L5; -.
DR OrthoDB; 6499288at2759; -.
DR Proteomes; UP000465112; Chromosome 6.
DR GO; GO:0009898; C:cytoplasmic side of plasma membrane; IEA:TreeGrafter.
DR GO; GO:0005829; C:cytosol; IEA:InterPro.
DR GO; GO:1990604; C:IRE1-TRAF2-ASK1 complex; IEA:UniProtKB-ARBA.
DR GO; GO:0043235; C:receptor complex; IEA:UniProtKB-ARBA.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IEA:UniProtKB-ARBA.
DR GO; GO:0005164; F:tumor necrosis factor receptor binding; IEA:UniProtKB-UniRule.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0043123; P:positive regulation of canonical NF-kappaB signal transduction; IEA:TreeGrafter.
DR GO; GO:0010628; P:positive regulation of gene expression; IEA:UniProtKB-ARBA.
DR GO; GO:0070534; P:protein K63-linked ubiquitination; IEA:UniProtKB-ARBA.
DR GO; GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
DR GO; GO:0043408; P:regulation of MAPK cascade; IEA:UniProtKB-ARBA.
DR GO; GO:0002700; P:regulation of production of molecular mediator of immune response; IEA:UniProtKB-ARBA.
DR GO; GO:1905669; P:TORC1 complex assembly; IEA:UniProtKB-ARBA.
DR GO; GO:1905670; P:TORC2 complex disassembly; IEA:UniProtKB-ARBA.
DR GO; GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IEA:InterPro.
DR CDD; cd16639; RING-HC_TRAF2; 1.
DR FunFam; 1.20.5.170:FF:000035; TNF receptor-associated factor; 1.
DR FunFam; 3.30.40.10:FF:000189; TNF receptor-associated factor; 1.
DR FunFam; 3.30.40.10:FF:000263; TNF receptor-associated factor; 1.
DR FunFam; 3.30.40.10:FF:000291; TNF receptor-associated factor; 1.
DR FunFam; 2.60.210.10:FF:000035; TNF receptor-associated factor 2; 1.
DR Gene3D; 1.20.5.170; -; 1.
DR Gene3D; 2.60.210.10; Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 3.
DR InterPro; IPR002083; MATH/TRAF_dom.
DR InterPro; IPR012227; TNF_rcpt-assoc_TRAF_met.
DR InterPro; IPR008974; TRAF-like.
DR InterPro; IPR049342; TRAF1-6_MATH_dom.
DR InterPro; IPR027133; TRAF2_RING-HC.
DR InterPro; IPR049441; TRAF2_Znf.
DR InterPro; IPR049440; TRAF3/5_RING.
DR InterPro; IPR032070; TRAF_BIRC3-bd.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR001293; Znf_TRAF.
DR PANTHER; PTHR10131; TNF RECEPTOR ASSOCIATED FACTOR; 1.
DR PANTHER; PTHR10131:SF160; TNF RECEPTOR-ASSOCIATED FACTOR; 1.
DR Pfam; PF21355; TRAF-mep_MATH; 1.
DR Pfam; PF21341; TRAF2_zf; 1.
DR Pfam; PF21363; TRAF3_RING; 1.
DR Pfam; PF16673; TRAF_BIRC3_bd; 1.
DR Pfam; PF02176; zf-TRAF; 1.
DR PIRSF; PIRSF015614; TRAF; 1.
DR SMART; SM00061; MATH; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR SUPFAM; SSF49599; TRAF domain-like; 2.
DR SUPFAM; SSF57953; Trimerization domain of TRAF; 1.
DR PROSITE; PS50144; MATH; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
DR PROSITE; PS50145; ZF_TRAF; 2.
PE 3: Inferred from homology;
KW Acetylation {ECO:0000256|ARBA:ARBA00022990};
KW Apoptosis {ECO:0000256|ARBA:ARBA00022703};
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|PIRNR:PIRNR015614};
KW Isopeptide bond {ECO:0000256|ARBA:ARBA00022499};
KW Lipid-binding {ECO:0000256|ARBA:ARBA00023121};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRNR:PIRNR015614};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000465112};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRNR:PIRNR015614};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00207}.
FT DOMAIN 35..73
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT DOMAIN 125..171
FT /note="TRAF-type"
FT /evidence="ECO:0000259|PROSITE:PS50145"
FT DOMAIN 178..234
FT /note="TRAF-type"
FT /evidence="ECO:0000259|PROSITE:PS50145"
FT DOMAIN 419..564
FT /note="MATH"
FT /evidence="ECO:0000259|PROSITE:PS50144"
FT ZN_FING 125..171
FT /note="TRAF-type"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00207"
FT ZN_FING 178..234
FT /note="TRAF-type"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00207"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 267..294
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 319..395
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1..10
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 569 AA; 64376 MW; E1BDCFB9075D7414 CRC64;
MAAQEPPSPP SSLEGNKPGF PKKILANNLE DKHLCNWCQK VLRRPLQAQC GHRFCSFCFN
NTVSSGPQKC NACIKEDLFE EPTSILKQGG AFPDNAARRE VEALAAVCPN EGCTWTGTIK
EFEASHEGHC DFMIILCPSC KELMRANEQE RHNERECPER TLNCKYCKEP FLLKNIKAHD
EICPKYPMIC EGCAKKKIPR EKYVDHIKFC SKFKAPCRFH VVGCDTSVEK ERIHDHERQC
SYEHLNLLLH FIMGIKVSLE SVQPQSLEVA GQRLQELHQS LRELELKMSQ LGRGAAPPVQ
GAGAPTLATS FTPLPSAAAA ALELQLHSEK TKVAELSRRC QELELKVGTF ENIVCVLNRE
MERSCTTMEA YNRQHRLDQD KIEILNNKVR QLERTVSLRD LSIVEMEGKM REMSAATYDG
IFVWKISDFT KKRQDAVAGR APAMFSPAFY TSKYGYKMCL RIYLNGDGTG RGTHLSLFFV
VMRGHSDALL KWPFNQKVTL MLLDQNNREH IIDAFRPDVS SSSFQRPVSD MNIASGCPLF
CPLSKLDSKN SYIRDDTIFI KAIVDLTGL
//
