ID A0A6A9T6E3_9EURY Unreviewed; 894 AA.
AC A0A6A9T6E3;
DT 17-JUN-2020, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 1.
DT 18-JUN-2025, entry version 18.
DE RecName: Full=DNA-directed RNA polymerase subunit Rpo1C {ECO:0000256|HAMAP-Rule:MF_00411};
DE EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_00411};
DE AltName: Full=DNA-directed RNA polymerase subunit A'' {ECO:0000256|HAMAP-Rule:MF_00411};
GN Name=rpo1C {ECO:0000256|HAMAP-Rule:MF_00411};
GN Synonyms=rpoA2 {ECO:0000256|HAMAP-Rule:MF_00411};
GN ORFNames=GJ633_04950 {ECO:0000313|EMBL:MUW14079.1};
OS Halorubrum sp. CBA1125.
OC Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
OC Halobacteria; Halobacteriales; Haloferacaceae; Halorubrum.
OX NCBI_TaxID=2668072 {ECO:0000313|EMBL:MUW14079.1, ECO:0000313|Proteomes:UP000432575};
RN [1] {ECO:0000313|EMBL:MUW14079.1, ECO:0000313|Proteomes:UP000432575}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBA1125 {ECO:0000313|EMBL:MUW14079.1,
RC ECO:0000313|Proteomes:UP000432575};
RA Roh S.W.;
RT "Genomic analysis of Halorubrum sp. CBA1125.";
RL Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC transcription of DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. Forms part of the jaw domain.
CC {ECO:0000256|HAMAP-Rule:MF_00411}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) +
CC diphosphate; Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00048552,
CC ECO:0000256|HAMAP-Rule:MF_00411};
CC -!- SUBUNIT: Part of the RNA polymerase complex. {ECO:0000256|HAMAP-
CC Rule:MF_00411}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00411}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|HAMAP-Rule:MF_00411}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:MUW14079.1}.
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DR EMBL; WPCE01000029; MUW14079.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A6A9T6E3; -.
DR OrthoDB; 372142at2157; -.
DR Proteomes; UP000432575; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR CDD; cd06528; RNAP_A; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR HAMAP; MF_00411; RNApol_arch_Rpo1C; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc_central.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_dom.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR012757; RPO1C.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR NCBIfam; NF011491; PRK14898.1; 1.
DR NCBIfam; TIGR02389; RNA_pol_rpoA2; 1.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF32; DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1; 1.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 2.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 2.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 2.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00411};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00411};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|HAMAP-Rule:MF_00411};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_00411}; Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Reference proteome {ECO:0000313|Proteomes:UP000432575};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00411};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00411}.
FT DOMAIN 287..417
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
SQ SEQUENCE 894 AA; 97391 MW; 8B7454B92D4AD68C CRC64;
MTDREHVTDD MEAVVEATEL PKRLKSEVYD AIERKAEETG EVTIEQATDI ATGVENRYLE
TRVDPLDPVG TVSAQSIGEP GTQMSVPADE RVVVRRNGET NVVEIGPLVD EVVDSRESRT
VDDHEVALAP DGLETLSLGD DEGVDWKQVE EVSRHKAPAE LLRFELESGR TIRATKAHSF
VTRRDNDVVP VAGEDLSAGD WLPVVASFGG GETSEIDLRE YLPATDYWYT STLADGGVDT
VPAGADQLRN KQNALESGRI EENAVYPAGG TVGLPERLPL DAETGFFFGA WLAEGSLTDH
YVSVSNVDET FQDGVRTFAD RFGLSVNEYE NESGFARGYD VRVNGTILAD VLRAACVEDG
EKVVPGFAFG ASGEFARGLL RGYFSGDGNV SDTAVRSSST SDRLTAGIAL LLARFDVYAT
LGRQDTSRTL RVPKKHVERF ADRIGMVGER GEQLDALASE LDGTGPDATD QIPNFGDALR
EVASDAGIPS RQVNAASNRQ RIGRTRLRRI LSEAAEAGVE SASLDALRQA VDGDVVWDRI
ASIETVTTGH EYVYDLSVEG LETFTTAEGV VTHNTMNTFH YAGVAEIDVT QGLPRLIELV
DARKTPDTPM MTVYLEDEYA TDREKAHEVV WSIEATRILA LGDVSTNVAD MLVRIDLNDD
TLLERWPTHS DPAEVAGIIA ETIEDALGVD ARQAGTVVEF GPNEPSYREL LQLVERLRDI
VFKGIEEIER VVIRKEQIDG EEEFVLYTEG SAFGDTLDIE GVDASRTTCN NIHEIYRNLG
VEAARETIID ETKNTLEEQG LDDVNVRHLM LVADIMTNNG EIESIGRHGI SGSKDSVLAR
AAFEVTVNHL LDAAIHGEYD ELDGVIENVI AGKPISLGTG DVDLRMGSRV VGDD
//
