ID A0A6I6EFP0_9GAMM Unreviewed; 306 AA.
AC A0A6I6EFP0; A0A6L6GMR2;
DT 12-AUG-2020, integrated into UniProtKB/TrEMBL.
DT 12-AUG-2020, sequence version 1.
DT 18-JUN-2025, entry version 19.
DE RecName: Full=2-dehydropantoate 2-reductase {ECO:0000256|ARBA:ARBA00019465, ECO:0000256|RuleBase:RU362068};
DE EC=1.1.1.169 {ECO:0000256|ARBA:ARBA00013014, ECO:0000256|RuleBase:RU362068};
DE AltName: Full=Ketopantoate reductase {ECO:0000256|ARBA:ARBA00032024, ECO:0000256|RuleBase:RU362068};
GN Name=panE {ECO:0000313|EMBL:QGU88677.1};
GN ORFNames=GN242_16240 {ECO:0000313|EMBL:QGU88677.1};
OS Erwinia sorbitola.
OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC Enterobacterales; Erwiniaceae; Erwinia.
OX NCBI_TaxID=2681984 {ECO:0000313|EMBL:QGU88677.1, ECO:0000313|Proteomes:UP000424752};
RN [1] {ECO:0000313|EMBL:QGU88677.1, ECO:0000313|Proteomes:UP000424752}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J780 {ECO:0000313|EMBL:QGU88677.1,
RC ECO:0000313|Proteomes:UP000424752};
RA Ge Y.;
RT "Erwinia sp. nov., isolated from droppings of birds in the Qinghai-Tiebt
RT plateau of China.";
RL Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the NADPH-dependent reduction of ketopantoate into
CC pantoic acid. {ECO:0000256|RuleBase:RU362068}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+);
CC Xref=Rhea:RHEA:16233, ChEBI:CHEBI:11561, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15980, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.1.1.169; Evidence={ECO:0000256|ARBA:ARBA00048793,
CC ECO:0000256|RuleBase:RU362068};
CC -!- PATHWAY: Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-
CC pantoate from 3-methyl-2-oxobutanoate: step 2/2.
CC {ECO:0000256|ARBA:ARBA00004994, ECO:0000256|RuleBase:RU362068}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the ketopantoate reductase family.
CC {ECO:0000256|ARBA:ARBA00007870, ECO:0000256|RuleBase:RU362068}.
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DR EMBL; CP046509; QGU88677.1; -; Genomic_DNA.
DR RefSeq; WP_154752388.1; NZ_CP046509.1.
DR AlphaFoldDB; A0A6I6EFP0; -.
DR KEGG; erwi:GN242_16240; -.
DR UniPathway; UPA00028; UER00004.
DR Proteomes; UP000424752; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter.
DR GO; GO:0008677; F:2-dehydropantoate 2-reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0050661; F:NADP binding; IEA:TreeGrafter.
DR GO; GO:0015940; P:pantothenate biosynthetic process; IEA:UniProtKB-UniPathway.
DR FunFam; 1.10.1040.10:FF:000014; 2-dehydropantoate 2-reductase; 1.
DR FunFam; 3.40.50.720:FF:000162; 2-dehydropantoate 2-reductase; 1.
DR Gene3D; 1.10.1040.10; N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR013328; 6PGD_dom2.
DR InterPro; IPR003710; ApbA.
DR InterPro; IPR050838; Ketopantoate_reductase.
DR InterPro; IPR013752; KPA_reductase.
DR InterPro; IPR013332; KPR_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR00745; apbA_panE; 1.
DR NCBIfam; NF005087; PRK06522.1-1; 1.
DR PANTHER; PTHR43765:SF2; 2-DEHYDROPANTOATE 2-REDUCTASE; 1.
DR PANTHER; PTHR43765; 2-DEHYDROPANTOATE 2-REDUCTASE-RELATED; 1.
DR Pfam; PF02558; ApbA; 1.
DR Pfam; PF08546; ApbA_C; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW NADP {ECO:0000256|ARBA:ARBA00022857, ECO:0000256|RuleBase:RU362068};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU362068};
KW Pantothenate biosynthesis {ECO:0000256|ARBA:ARBA00022655,
KW ECO:0000256|RuleBase:RU362068}.
SQ SEQUENCE 306 AA; 33720 MW; 7D685DD81E76612A CRC64;
MKITVLGCGA LGQIWLAALE QQGHEVQGWL RVPQPYCSVN VIGLNGETLN KTFIANDPAF
LGDSELLIVT LKAWQVSEAV KNMQSVLPAH CPVLLLHNGM GTLDELQGLP QPLLRGVTTH
AARRDGTVIV HVASGITHIG PVSAAGEDMS ELAEILHRAL PDVAWHNNVA SAAWQKLAVN
CVINPLSVFY NCTNGELAEH RQEIEALSNE VAAVMEREGQ HTSREWLIDY VLEVIRSTAG
NTSSMLQDIR AQRRTEIDYI NGYVIRRGRA QGISSPENSR LYEFIKRKEQ DYDRESIGSG
LPGTWE
//
