ID A0A6I9Q0A4_9TELE Unreviewed; 2338 AA.
AC A0A6I9Q0A4;
DT 07-OCT-2020, integrated into UniProtKB/TrEMBL.
DT 07-OCT-2020, sequence version 1.
DT 18-JUN-2025, entry version 22.
DE RecName: Full=Pre-mRNA-processing-splicing factor 8 {ECO:0000256|ARBA:ARBA00071757};
DE AltName: Full=Splicing factor Prp8 {ECO:0000256|ARBA:ARBA00079260};
GN Name=prpf8 {ECO:0000313|RefSeq:XP_010794252.1,
GN ECO:0000313|RefSeq:XP_010794253.1};
OS Notothenia coriiceps (black rockcod).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Notothenioidei; Nototheniidae; Notothenia.
OX NCBI_TaxID=8208 {ECO:0000313|Proteomes:UP000504611, ECO:0000313|RefSeq:XP_010794252.1};
RN [1] {ECO:0000313|RefSeq:XP_010794252.1, ECO:0000313|RefSeq:XP_010794253.1}
RP IDENTIFICATION.
RC TISSUE=Muscle {ECO:0000313|RefSeq:XP_010794252.1,
RC ECO:0000313|RefSeq:XP_010794253.1};
RG RefSeq;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- FUNCTION: Plays a role in pre-mRNA splicing as core component of
CC precatalytic, catalytic and postcatalytic spliceosomal complexes, both
CC of the predominant U2-type spliceosome and the minor U12-type
CC spliceosome. Functions as a scaffold that mediates the ordered assembly
CC of spliceosomal proteins and snRNAs. Required for the assembly of the
CC U4/U6-U5 tri-snRNP complex, a building block of the spliceosome.
CC Functions as a scaffold that positions spliceosomal U2, U5 and U6
CC snRNAs at splice sites on pre-mRNA substrates, so that splicing can
CC occur. Interacts with both the 5' and the 3' splice site.
CC {ECO:0000256|ARBA:ARBA00055739}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR RefSeq; XP_010794252.1; XM_010795950.1.
DR RefSeq; XP_010794253.1; XM_010795951.1.
DR GeneID; 104966717; -.
DR KEGG; ncc:104966717; -.
DR CTD; 10594; -.
DR OrthoDB; 1931567at2759; -.
DR Proteomes; UP000504611; Unplaced.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IEA:TreeGrafter.
DR GO; GO:0005682; C:U5 snRNP; IEA:TreeGrafter.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IEA:TreeGrafter.
DR GO; GO:0030619; F:U1 snRNA binding; IEA:TreeGrafter.
DR GO; GO:0030620; F:U2 snRNA binding; IEA:TreeGrafter.
DR GO; GO:0030623; F:U5 snRNA binding; IEA:InterPro.
DR GO; GO:0017070; F:U6 snRNA binding; IEA:InterPro.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IEA:TreeGrafter.
DR CDD; cd08056; MPN_PRP8; 1.
DR CDD; cd13838; RNase_H_like_Prp8_IV; 1.
DR FunFam; 1.20.80.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.30.420.230:FF:000003; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.30.43.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.40.140.10:FF:000002; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.90.1570.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR Gene3D; 1.20.80.40; -; 1.
DR Gene3D; 3.30.420.230; -; 1.
DR Gene3D; 3.90.1570.40; -; 1.
DR Gene3D; 3.40.140.10; Cytidine Deaminase, domain 2; 1.
DR Gene3D; 3.30.43.40; Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain; 1.
DR InterPro; IPR000555; JAMM/MPN+_dom.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR012591; PRO8NT.
DR InterPro; IPR012592; PROCN.
DR InterPro; IPR012984; PROCT.
DR InterPro; IPR027652; PRP8.
DR InterPro; IPR021983; PRP8_domainIV.
DR InterPro; IPR043173; Prp8_domainIV_fingers.
DR InterPro; IPR043172; Prp8_domainIV_palm.
DR InterPro; IPR019581; Prp8_U5-snRNA-bd.
DR InterPro; IPR042516; Prp8_U5-snRNA-bd_sf.
DR InterPro; IPR019580; Prp8_U6-snRNA-bd.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR019582; RRM_spliceosomal_PrP8.
DR PANTHER; PTHR11140; PRE-MRNA SPLICING FACTOR PRP8; 1.
DR PANTHER; PTHR11140:SF0; PRE-MRNA-PROCESSING-SPLICING FACTOR 8; 1.
DR Pfam; PF01398; JAB; 1.
DR Pfam; PF08082; PRO8NT; 1.
DR Pfam; PF08083; PROCN; 1.
DR Pfam; PF08084; PROCT; 1.
DR Pfam; PF12134; PRP8_domainIV; 1.
DR Pfam; PF10598; RRM_4; 1.
DR Pfam; PF10597; U5_2-snRNA_bdg; 1.
DR Pfam; PF10596; U6-snRNA_bdg; 1.
DR SMART; SM00232; JAB_MPN; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 2.
DR PROSITE; PS50249; MPN; 1.
PE 4: Predicted;
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000504611};
KW Ribonucleoprotein {ECO:0000256|ARBA:ARBA00023274};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Spliceosome {ECO:0000256|ARBA:ARBA00022728}.
FT DOMAIN 2106..2237
FT /note="MPN"
FT /evidence="ECO:0000259|PROSITE:PS50249"
SQ SEQUENCE 2338 AA; 273975 MW; 2301AC4232ED69BC CRC64;
MAATFPYRGV PGGMPPGVPP PIPVPDYMSE EKLQEKARKW QHLQAKRYSE KRKFGFVDAQ
KEDMPPEHVR KIIRDHGDMT NRKFRHDKRV YLGALKYMPH AVLKLLENMP MPWEQIRDVP
VLYHITGAIS FVNEIPWVIE PVYIAQWGTM WIMMRREKRD RRHFKRMRFP PFDDEEPPLD
YADNILDVEP LEAIQMELDN EEDSPVAEWL YDHQPLKDAN KFVNGTTYRR WQFTLPMMST
LYRLANQLLT DLVDFNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN
DINKIIIRQP IRTEYKIAFP YLYNNLPHYV HLTWYHTPNV VFIKTEDPDL PAFYFDPLIN
PISHRHSVKS QEPLPDDDEE FELPEYVEPL LKETPLYTDN TANGIALLWA PRPFNLRSGR
TRRAIDIPLI KNWYREHCPA GQPVKVRVSY QKLLKYYVLN ALKHRPPKAQ KKRYLFRSFK
ATKFFQSTKL DWVEVGLQVC RQGYNMLNLL IHRKNLNYLH LDYNFNLKPV KTLTTKERKK
SRFGNAFHLC REVLRLSKLV VDSHVQYRLG NVDAFQLSDG LQYIFAHVGQ LTGMYRYKYK
LMRQIRMCKD LKHLIYYRFN TGPVGKGPGC GFWAPGWRVW LFFMRGITPL LERWLGNLLA
RQFEGRHSKG VAKTVTKQRV ESHFDLELRA AVMHDILDMM PEGIKQNKAR TILQHLSESW
RCWKANIPWK VPGLPTPIEN MILRYVKAKA DWWTNTAHYN RERIRRGATV DKTVCKKNLG
RLTRLYLKAE QERQHNYLKD GPYITAEEAV AIYTTTVHWL ESRRFSPIPF PPLSYKHDTK
LLILALERLK EAYSVKSRLN QSQREELGLI EQAYDNPHEA LSRIKRHLLT QRAFKEVGIE
FMDLYSHLVP VYDVEPLEKI TDAYLDQYLW YEADKRRLFP PWIKPADTEP PPLLVYKWCQ
GINNLQDVWE TAEGECNVML ESRYEKMYEK IDLTLLNRLL RLIVDHNIAD YMTAKNNVVI
NYKDMNHTNS YGIIRGLQFA SFIVQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDT
PTESAHPIRL YCRYIDRIHI FFRFSADEAR DLIQRYLTEH PDPNNENIVG YNNKKCWPRD
ARMRLMKHDV NLGRAVFWDI KNRLPRSVTT VQWENSFVSV YSKDNPNLLF NMCGFECRIL
PKCRTSYEEF THKDGVWNLQ NEVTKERTAQ CFLRVDDESM QRFHNRVRQI LMASGSTTFT
KIVNKWNTAL IGLMTYFREA VVNTQELLDL LVKCENKIQT RIKIGLNSKM PSRFPPVVFY
TPKELGGLGM LSMGHVLIPQ SDLRWSKQTD VGITHFRSGM SHEEDQLIPN LYRYIQPWES
EFIDSQRVWA EYALKRQEAI AQNRRLTLED LEDSWDRGIP RINTLFQKDR HTLAYDKGWR
VRTDFKQYQV LKQNPFWWTH QRHDGKLWNL NNYRTDMIQA LGGVEGILEH TLFKGTYFPT
WEGLFWEKAS GFEESMKWKK LTNAQRSGLN QIPNRRFTLW WSPTINRANV YVGFQVQLDL
TGIFMHGKIP TLKISLIQIF RAHLWQKIHE SVVMDLCQVF DQELDALEIE TVQKETIHPR
KSYKMNSSCA DILLFASYKW NVSRPSLLAD SKDVMDSTTT QKYWIDIQLR WGDYDSHDIE
RYARAKFLDY TTDNMSIYPS PTGVLIAIDL AYNLHSAYGN WFPGSKPLIQ QAMAKIMKAN
PALYVLRERI RKGLQLYSSE PTEPYLSSQN YGELFSNQII WFVDDTNVYR VTIHKTFEGN
LTTKPINGAI FIFNPRTGQL FLKIIHTSVW AGQKRLGQLA KWKTAEEVAA LIRSLPVEEQ
PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF
NLYDDWLKTI SSYTAFSRLI LILRALHVNN DRAKVILKPD KTTITEPHHI WPTLTDEEWI
KVEVQLKDLI LADYGKKNNV NVASLTQSEI RDIILGMEIS APSQQRQQIA EIEKQTKEQS
QLTATQTRTV NKHGDEIITS TTSNYETQTF SSKTEWRVRA ISAANLHLRT NHIYVSSDDI
KETGYTYILP KNVLKKFICI SDLRAQIAGY LYGTSPPDNP QVKEIRCIVM VPQWGTHQTV
HLPNQLPGHE YLKEMEPLGW IHTQPNESPQ LSPQDVTTHA KVMADNPSWD GEKTIIITCS
FTPGSCTLTA YKLTPSGYEW GRQNTDKGNN PKGYLPSHYE RVQMLLSDRF LGFFMVPGQV
SWNYNFMGVR HDPNMKYDLQ LANPMEFYHE VHRPSHFLNF ASLQEGEVYN ADREDMYA
//
