ID A0A6J0JKI3_RAPSA Unreviewed; 455 AA.
AC A0A6J0JKI3;
DT 07-OCT-2020, integrated into UniProtKB/TrEMBL.
DT 07-OCT-2020, sequence version 1.
DT 18-JUN-2025, entry version 22.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN Name=LOC108808510 {ECO:0000313|RefSeq:XP_018436142.1,
GN ECO:0000313|RefSeq:XP_056845561.1};
OS Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus.
OX NCBI_TaxID=3726 {ECO:0000313|Proteomes:UP000504610, ECO:0000313|RefSeq:XP_018436142.1};
RN [1] {ECO:0000313|Proteomes:UP000504610}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. WK10039 {ECO:0000313|Proteomes:UP000504610};
RX PubMed=30722041;
RA Yu H.J., Baek S., Lee Y.J., Cho A., Mun J.H.;
RT "The radish genome database (RadishGD): an integrated information resource
RT for radish genomics.";
RL Database 2019:5306501-5306501(2019).
RN [2] {ECO:0000313|RefSeq:XP_018436142.1, ECO:0000313|RefSeq:XP_056845561.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_018436142.1,
RC ECO:0000313|RefSeq:XP_056845561.1};
RG RefSeq;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR RefSeq; XP_018436142.1; XM_018580640.2.
DR RefSeq; XP_056845561.1; XM_056989581.1.
DR GeneID; 108808510; -.
DR KEGG; rsz:108808510; -.
DR OrthoDB; 10009520at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000504610; Chromosome 6.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd20346; BRcat_RBR_ANKIB1; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 1.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
PE 4: Predicted;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000504610};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 72..300
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
SQ SEQUENCE 455 AA; 51988 MW; D75E67B90B9C51A4 CRC64;
METDGQRVYS VLTTNEVGEK MMSEINQISE VFLLSKSDAT VILISFHWNS FKASDRLGDD
KEKLLLELGL VKVFSSSQEN KSVPSFADQE NLVSTPFCPH KFSETCWISH LNNLLEEGKA
ENNKEEERVL CPNPDCVASV GPDTIEKLKE PAVKMMYERY VLRSFVEANK EWIKWCPAPG
CEYAIERHED MLEDDDESSL DFGVVCLCGH TFCWCCKLES HRPLTCKDAS VWFSSTLDTL
KSNAWLVENT KRCPRCNTHV QRADEPVLRM ITCFCSCHFC WRCFRSAKEH NGKWNCVEVS
VEPSMRQEVE DSRYLRLWEA CHEEFEKSKS DLKAIEENGI PTLVENFSFN NGDVDAVMEA
TLLMVQCRLA LKWSCAYEYL ITMHQSAKTQ FVNHLRGEAT KTLLRHKATQ EALMNQALTS
GDFSCFEVKL SASTTTTGNY FHEFVKTLES GFPGT
//
