ID A0A6J1SQQ0_FRAOC Unreviewed; 471 AA.
AC A0A6J1SQQ0;
DT 07-OCT-2020, integrated into UniProtKB/TrEMBL.
DT 07-OCT-2020, sequence version 1.
DT 10-JUN-2026, entry version 24.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Name=LOC113209763 {ECO:0000313|RefSeq:XP_026283227.1};
OS Frankliniella occidentalis (Western flower thrips) (Euthrips occidentalis).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Paraneoptera; Thysanoptera; Terebrantia; Thripoidea; Thripidae;
OC Frankliniella.
OX NCBI_TaxID=133901 {ECO:0000313|Proteomes:UP000504606, ECO:0000313|RefSeq:XP_026283227.1};
RN [1] {ECO:0000313|RefSeq:XP_026283227.1}
RP IDENTIFICATION.
RC TISSUE=Whole organism {ECO:0000313|RefSeq:XP_026283227.1};
RG RefSeq;
RL Submitted (JAN-2026) to UniProtKB.
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
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DR RefSeq; XP_026283227.1; XM_026427442.2.
DR AlphaFoldDB; A0A6J1SQQ0; -.
DR GeneID; 113209763; -.
DR CTD; 348180; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000504606; Unplaced.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000504606};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 471 AA; 52565 MW; 1EDD567FED8B009A CRC64;
MCSRNDEEDG SQSLMEGKTK LTWTEVCQKC STEKPVIQIL QIKHAYCRNC FLAHVNHKFR
STLGKSKMMK PSDVVLAAFS GSGKSNAMLH LLHAGLSEQS HKRFLFKVQV LYIDEGAILG
TSLEDRTANL QAISGIIEQY GFKSYYTTLA SIFEETGSKC FPLVKDSTLK IKENSEEELK
NLFLNVKSLT AREDLLRQLR RKIFLQSAKE LDCCKVFTAN TSTDLAIHIL TDVSLGRGSQ
MHHDVSFCDP RDASVKIIRP LLELSSKEVT YYSVFHELES IYIPSLCTKA SSDSSIHNMS
KQFVMELMEN FPSTVSTVFR TGEKLGSTNT TNTCETEDNL CIICQMKLDV SVAACSSVQA
TDFSRLISSL GPAGFDNKTL SQLPGILSNQ ADSLLCSSST SCCSDSDVQC GCHKDVQRLS
FPDVEKLCCY GCRLIIHDME NTSSLPESIL HKVRTRRSLE NMRESIQDFL L
//
