ID A0A6J2P8E0_COTGO Unreviewed; 1141 AA.
AC A0A6J2P8E0;
DT 07-OCT-2020, integrated into UniProtKB/TrEMBL.
DT 07-OCT-2020, sequence version 1.
DT 18-JUN-2025, entry version 23.
DE RecName: Full=mitogen-activated protein kinase kinase kinase {ECO:0000256|ARBA:ARBA00012406};
DE EC=2.7.11.25 {ECO:0000256|ARBA:ARBA00012406};
GN Name=map3k5 {ECO:0000313|RefSeq:XP_029281636.1};
OS Cottoperca gobio (Frogmouth) (Aphritis gobio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Perciformes; Notothenioidei; Bovichtidae; Cottoperca.
OX NCBI_TaxID=56716 {ECO:0000313|Proteomes:UP000504630, ECO:0000313|RefSeq:XP_029281636.1};
RN [1] {ECO:0000313|RefSeq:XP_029281636.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP +
CC H(+); Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.25;
CC Evidence={ECO:0000256|ARBA:ARBA00048329};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] +
CC ADP + H(+); Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.25;
CC Evidence={ECO:0000256|ARBA:ARBA00047559};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr
CC protein kinase family. MAP kinase kinase kinase subfamily.
CC {ECO:0000256|ARBA:ARBA00006529}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR RefSeq; XP_029281636.1; XM_029425776.1.
DR AlphaFoldDB; A0A6J2P8E0; -.
DR GeneID; 115003836; -.
DR Proteomes; UP000504630; Chromosome 24.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004709; F:MAP kinase kinase kinase activity; IEA:TreeGrafter.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0033554; P:cellular response to stress; IEA:TreeGrafter.
DR CDD; cd06624; STKc_ASK; 1.
DR FunFam; 1.10.510.10:FF:000054; Mitogen-activated protein kinase kinase kinase 5; 1.
DR FunFam; 3.30.200.20:FF:000067; Mitogen-activated protein kinase kinase kinase 5; 1.
DR Gene3D; 3.30.200.20; Phosphorylase Kinase, domain 1; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR046872; DRHyd-ASK.
DR InterPro; IPR046873; HisK-N-like.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR043969; MAP3K_PH.
DR InterPro; IPR025136; MAP3K_TRAF-bd.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR11584:SF332; MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5; 1.
DR PANTHER; PTHR11584; SERINE/THREONINE PROTEIN KINASE; 1.
DR Pfam; PF19039; ASK_PH; 1.
DR Pfam; PF20309; DRHyd-ASK; 1.
DR Pfam; PF20302; HisK-N-like; 1.
DR Pfam; PF13281; MAP3K_TRAF_bd; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Coiled coil {ECO:0000256|ARBA:ARBA00023054};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|RefSeq:XP_029281636.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Reference proteome {ECO:0000313|Proteomes:UP000504630};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 677..935
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT BINDING 706
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 1141 AA; 127505 MW; 2D4B3AA7B363CC3A CRC64;
MISQDQDGIS LPVPCFGVCP SRESLGGESS SSSSGGGGGG GVYRIPAAGT FWQDSVIIGG
VLSPTPGTNP MDGGGLLPTG KSCKSRPVTV AYVVNGEASQ QNNAESMALQ CLKDACETSS
SKLETVNFGK LDFGETTVLD SFYNADIAVV EMTDGFRQPS LFYHLGVRES FSMANNIILY
CDTNADSLQS LQDIICHKNT TCSANYTFIP YMVTPHNKVY CCESSLMKGL TELMQPSFEM
LLGPICMPLL DRFIQLLKVS QANSHQYFRE TILNEIRKAR ELYTGMELAT ELSRIQQRLD
NVECLSVDIV INLLLTYRDI QDYESIVKLV ETLEKLPTFD PVAHPHVKFH YAFALNRRNL
PGDRQKALDI MLPLVKGEEQ VASDIYCLVG RIYKDMFLES HFTDTPSRDS GTQWFKKGFE
SEPTLHSGIN YAVLLLAAGH QFDSSLELRK VGVKLSSLLG KKGSLDKLQS YWDVGFFLGA
SILACDNTRV IQASEKLFKL KAPIWYLRSL VETILIYQQF KKPGAEQPAP KQELVDFWMD
FLVEATKKDV SSVRFPVLIL EPTKVYQPSY LSINKDVDDN TVSIWHVAAD DKHKGIHEWN
FSATSVRGVS ISKFDERSAF LYVLHNAEDF QIYFCTEMHC KRFCDLVNSI TEEAWKGPEE
GDCDADALEY DYEYDELAER VVLGKGTFGV VYAGRDLSNQ VRLAIKEIPE RDSRYSQPLH
EEIALHKHLK HKNIVQYLGS ISENGFIKIF MEQVPGGSLS ALLRSKWGPL KNNEHTIGFY
TRQILEGLKY LHDNQIAHRD IKGDNVLINT YSGVLKISDF GTSKRLAGIN PCTETFTGTL
QYMAPEIIDK GPRGYGKPAD IWSLGCTIIE MATGKPPFYE LGEPQAAMFK VGMFKIHPEI
PESMSLEAKA FILRCFEPDP DRRATAFDLL TDEFLTVTSR KKKSKSSFTA LSPGSEYLRS
ISLPVPVVVE DTSSSSEYGS VSPDNDLNTN PFIFKPSVKC YSERDVKGPR SLFLSIPVEN
FEDHSAPPSP DEKDSGFFML RKDSERRATL HHILTEDRDK VVANLMEAIT QGSEEMKLKP
QHISTLVVSL ADFVRMADRK IIANTLSQLK LELDFDSTAI SQLQIVLFAF QDAAELLPSS
S
//
