ID A0A6J3LI80_9HYME Unreviewed; 713 AA.
AC A0A6J3LI80;
DT 07-OCT-2020, integrated into UniProtKB/TrEMBL.
DT 07-OCT-2020, sequence version 1.
DT 28-JAN-2026, entry version 27.
DE RecName: Full=Vesicle-fusing ATPase {ECO:0000256|ARBA:ARBA00012674, ECO:0000256|RuleBase:RU367045};
DE EC=3.6.4.6 {ECO:0000256|ARBA:ARBA00012674, ECO:0000256|RuleBase:RU367045};
GN Name=LOC117242489 {ECO:0000313|RefSeq:XP_033365092.1};
OS Bombus vosnesenskii.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea;
OC Anthophila; Apidae; Bombus; Pyrobombus.
OX NCBI_TaxID=207650 {ECO:0000313|Proteomes:UP000504631, ECO:0000313|RefSeq:XP_033365092.1};
RN [1] {ECO:0000313|RefSeq:XP_033365092.1}
RP IDENTIFICATION.
RC TISSUE=Muscle {ECO:0000313|RefSeq:XP_033365092.1};
RG RefSeq;
RL Submitted (AUG-2025) to UniProtKB.
CC -!- FUNCTION: Required for vesicle-mediated transport. Catalyzes the fusion
CC of transport vesicles within the Golgi cisternae. Is also required for
CC transport from the endoplasmic reticulum to the Golgi stack. Seems to
CC function as a fusion protein required for the delivery of cargo
CC proteins to all compartments of the Golgi stack independent of vesicle
CC origin. {ECO:0000256|RuleBase:RU367045}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.6;
CC Evidence={ECO:0000256|ARBA:ARBA00048883,
CC ECO:0000256|RuleBase:RU367045};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU367045};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|RuleBase:RU367045};
CC -!- SUBUNIT: Homohexamer. {ECO:0000256|ARBA:ARBA00011643}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU367045}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family.
CC {ECO:0000256|ARBA:ARBA00006914, ECO:0000256|RuleBase:RU367045}.
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DR RefSeq; XP_033365092.1; XM_033509201.1.
DR AlphaFoldDB; A0A6J3LI80; -.
DR GeneID; 117242489; -.
DR Proteomes; UP000504631; Unplaced.
DR GO; GO:0005795; C:Golgi stack; IEA:TreeGrafter.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IEA:TreeGrafter.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IEA:TreeGrafter.
DR GO; GO:0035494; P:SNARE complex disassembly; IEA:InterPro.
DR CDD; cd19504; RecA-like_NSF-SEC18_r1-like; 1.
DR FunFam; 1.10.8.60:FF:000026; vesicle-fusing ATPase isoform X1; 1.
DR FunFam; 1.10.8.60:FF:000031; vesicle-fusing ATPase isoform X1; 1.
DR FunFam; 3.40.50.300:FF:000166; vesicle-fusing ATPase isoform X1; 1.
DR FunFam; 3.40.50.300:FF:000187; Vesicular-fusion ATPase SEC18; 1.
DR Gene3D; 1.10.8.60; -; 2.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.10.330.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR004201; Cdc48_dom2.
DR InterPro; IPR029067; CDC48_domain_2-like_sf.
DR InterPro; IPR054419; NSF_ATPase_lid.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR039812; Vesicle-fus_ATPase.
DR PANTHER; PTHR23078:SF3; VESICLE-FUSING ATPASE; 1.
DR PANTHER; PTHR23078; VESICULAR-FUSION PROTEIN NSF; 1.
DR Pfam; PF00004; AAA; 2.
DR Pfam; PF17862; AAA_lid_3; 1.
DR Pfam; PF02933; CDC48_2; 1.
DR Pfam; PF21964; NSF_ATPase_lid; 1.
DR SMART; SM00382; AAA; 2.
DR SMART; SM01072; CDC48_2; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF54585; Cdc48 domain 2-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00674; AAA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU367045};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU367045};
KW ER-Golgi transport {ECO:0000256|RuleBase:RU367045};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU367045};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU367045};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU367045};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU367045};
KW Protein transport {ECO:0000256|ARBA:ARBA00022927,
KW ECO:0000256|RuleBase:RU367045};
KW Reference proteome {ECO:0000313|Proteomes:UP000504631};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU367045}.
FT DOMAIN 84..156
FT /note="CDC48"
FT /evidence="ECO:0000259|SMART:SM01072"
FT DOMAIN 225..372
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 508..644
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
SQ SEQUENCE 713 AA; 79469 MW; 91FA1A50998741F1 CRC64;
MKHTHIEVTT APNHHFVFTI KRHHEVPRGT VGFSLLQRKW ATLSLNQEIE VRPYHFNPTS
STECLCTIVL EADFLQKKST TLEPYNTDEM AKDFLLQFSG QAFTVGQQLV FQFKDKRMLG
LVVKSLEAAD ISAISSGQNT VSKKTQMGRC LGDTVIQFEK AENSNVNLIG KAKGNVIRQS
IINPDWDFQK MGIGGLDNEF SAIFRRAFAS RVFPPEIVTQ LGCKHVKGIL LYGPPGTGKT
LMARQIGTML NAREPKIVNG PQILDKYVGE SEANVRRLFA DAEEEEKRLG PNSGLHIIIF
DEIDAICKSR GSVAGNTGVH DTVVNQLLAK IDGVEQLNNI LVIGMTNRRD MIDEALLRPG
RLEVQMEISL PDEHGRFQIL NIHTSRMKDY KKISPDVDLR ELATLTKNFS GAELEGLVRA
AQSTAMNRLI KASSKVEVDP AAMEKLMVSR TDFLHALEHD VKPAFGTSAE KLDHLLIRGI
INWGKPVAEI LSDGNLYIQE ARSAEGSGLV SVLLEGPPNS GKTALAAQIA KNSDFPFVKV
CTPEDMVGFT ESAKCLLIRK IFDDAYRSQL SCILVDNIER LLDYGSIGPR YSNLTLQALL
VLLKKQPPRG RKLLVLCTTS RRQVLEDMEM LSAFSTTLHV PNLSTPDHLL SVLEEMDLFN
KKEIVHLHAK LQGKRVFIGI KKLLCLIDMA RQVEPKYRVP KFLSKLEEEG GLE
//
