ID A0A6M4GQE8_9PROT Unreviewed; 635 AA.
AC A0A6M4GQE8;
DT 07-OCT-2020, integrated into UniProtKB/TrEMBL.
DT 07-OCT-2020, sequence version 1.
DT 28-JAN-2026, entry version 23.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:QJR09286.1};
GN ORFNames=DSM104443_00323 {ECO:0000313|EMBL:QJR09286.1};
OS Usitatibacter rugosus.
OC Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
OC Nitrosomonadales; Usitatibacteraceae; Usitatibacter.
OX NCBI_TaxID=2732067 {ECO:0000313|EMBL:QJR09286.1, ECO:0000313|Proteomes:UP000501534};
RN [1] {ECO:0000313|EMBL:QJR09286.1, ECO:0000313|Proteomes:UP000501534}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0125_3 {ECO:0000313|EMBL:QJR09286.1,
RC ECO:0000313|Proteomes:UP000501534};
RA Huber K.J., Neumann-Schaal M., Geppert A., Luckner M., Wanner G.,
RA Overmann J.;
RT "Usitatibacter rugosus gen. nov., sp. nov. and Usitatibacter palustris sp.
RT nov., novel members of Usitatibacteraceae fam. nov. within the order
RT Nitrosomonadales isolated from soil.";
RL Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; CP053069; QJR09286.1; -; Genomic_DNA.
DR RefSeq; WP_171088989.1; NZ_CP053069.1.
DR AlphaFoldDB; A0A6M4GQE8; -.
DR KEGG; uru:DSM104443_00323; -.
DR Proteomes; UP000501534; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR CDD; cd04171; SelB; 1.
DR CDD; cd03696; SelB_II; 1.
DR CDD; cd15491; selB_III; 1.
DR Gene3D; 1.10.10.2770; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR050055; EF-Tu_GTPase.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR015190; Elong_fac_SelB-wing-hlx_typ-2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR048931; WHD_2nd_SelB_bact.
DR NCBIfam; TIGR00475; selB; 1.
DR PANTHER; PTHR43721:SF22; ELONGATION FACTOR TU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF09106; WHD_2nd_SelB; 1.
DR Pfam; PF21214; WHD_2nd_SelB_bact; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 3.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Elongation factor {ECO:0000313|EMBL:QJR09286.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000501534}.
FT DOMAIN 1..169
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 635 AA; 68554 MW; 061B3F0F17523BCF CRC64;
MIVGTAGHID HGKTSLVKAL TGVDTDRLKE EKARGISIEL GYAYKPLPGG AILGFVDVPG
HEKFVDHMVA GATGIDFVLL VIAADDGPMP QTLEHLDIVS LLGVRTGAVA MTKVDRVDAT
RRAECEREIR ALLSGGPLAD APIFGVSSVT REGLDALQAH LQEAAAAQDA RRPGAGFRLA
VDRCFTLDGI GTVVTGTVFS GAVTVGDEVI VSPSGERTRV RSLHAQNRTA TVGHAGQRCA
LALGGIAKEA IARGDWIVAP PLHLPTARID VRLRLSAREA KAFRHWTAVH LHLGAAHALA
RVALLEGESL APGSEALAQL VVGQPIGAWA GDAFIVRDAS GARTLGGGVV VDPVGRERHR
RAPERLDTLR RLEAPTPAAR LHALLEASDT GIDLDQFRAT QNVAGSELEL GRDVIRIESG
GRDIAIGAAP WQRLRDTFVE RLARSHEERP EELGPDLGRV RRMAFPRHRS AVVDALAASL
LEEGRVARSG PWWHLPTHSI RLTEREEQLA RAILPRLDAE GVDPTWVRDH AKAVGAPEHE
VRALMRRLGR RGDVFAVVKD LYFSRSAVER LALAAKALDD ESGAVRAAEF RDRIGIGRKR
AIQILEFFDR VGFTRRAHDE HRVRGDSLLR LGESP
//
