ID A0A6N0DUI7_9HYPH Unreviewed; 695 AA.
AC A0A6N0DUI7;
DT 07-OCT-2020, integrated into UniProtKB/TrEMBL.
DT 07-OCT-2020, sequence version 1.
DT 18-JUN-2025, entry version 21.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00044770};
DE EC=2.4.99.28 {ECO:0000256|ARBA:ARBA00044770};
GN Name=pbpC {ECO:0000313|EMBL:QKP78599.1};
GN ORFNames=HT051_14840 {ECO:0000313|EMBL:QKP78599.1};
OS Methyloligella sp. GL2.
OC Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC Hyphomicrobiales; Hyphomicrobiaceae; Methyloligella.
OX NCBI_TaxID=2742204 {ECO:0000313|EMBL:QKP78599.1, ECO:0000313|Proteomes:UP000509225};
RN [1] {ECO:0000313|EMBL:QKP78599.1, ECO:0000313|Proteomes:UP000509225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GL2 {ECO:0000313|EMBL:QKP78599.1,
RC ECO:0000313|Proteomes:UP000509225};
RA Grouzdev D.S.;
RL Submitted (JUN-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans,octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.99.28;
CC Evidence={ECO:0000256|ARBA:ARBA00049902};
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004752}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the transpeptidase
CC family. {ECO:0000256|ARBA:ARBA00007090}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the
CC glycosyltransferase 51 family. {ECO:0000256|ARBA:ARBA00007739}.
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DR EMBL; CP054476; QKP78599.1; -; Genomic_DNA.
DR RefSeq; WP_174542748.1; NZ_CP054476.1.
DR AlphaFoldDB; A0A6N0DUI7; -.
DR KEGG; metg:HT051_14840; -.
DR UniPathway; UPA00219; -.
DR Proteomes; UP000509225; Chromosome.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:TreeGrafter.
DR GO; GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:InterPro.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR050396; Glycosyltr_51/Transpeptidase.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR011815; PBP_1c.
DR InterPro; IPR009647; PBP_C.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR NCBIfam; TIGR02073; PBP_1c; 1.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF15; PENICILLIN-BINDING PROTEIN 1C; 1.
DR Pfam; PF06832; BiPBP_C; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 3: Inferred from homology;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022670};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000509225};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT DOMAIN 69..235
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 312..528
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
FT DOMAIN 608..690
FT /note="Penicillin-binding C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06832"
SQ SEQUENCE 695 AA; 74074 MW; BEB90F4707602751 CRC64;
MTRRLRPSRL RRALLYSGLG VLFAGAIAGG AVSGFVETLG PAPLGDQVEM SPIVVDREGR
LLRPYTTKAG RWRLPATVAD VDPRFLDVLL AYEDKRFYAH PGVDPLAMLR AAEQLATQGH
IVSGGSTLTM QVARLLEPRP RSFEAKARQI VRAIQMERKL GKQQVLSRYL TLAPYGGNLE
GIRAASLAYF GKEPRRLSLG ESALLVALPQ SPEERRPDRS PKAAQAARDR VLDRIAGSGV
FSNAEIARAR LEPVPHARKP MPILAPHAAD DAVAAAPETR IHRLTIDKRM QARLEQLAKE
RADRLGPDIS VAMVALDNAT GAVLARVGTP DYFDKRRAGQ VDLTRAVRSP GSALKPFIYG
LGFEDGAIHP ATLIKDKPVR FGAYAPENFD MSYQGTVSIR RALQLSLNVP AIAVLDAVGP
ERLVARLGEV GTPLELPPHE KPGLALGLGG VGVTLSALTQ GYSGIARQGS VMASYERQDQ
PVPAPKRLIS PVAAWYVADI LRGAPPPENG LPGRIAFKTG TSYGYRDAWS IGFDGARTIG
VWVGRPDGAP VPGLVGRLSA APILFDAFAR TGPVTPLPPA PDGVLTASTA ELPRPLQRFT
TDPVTAGSTE QALRILFPPN GASLELSGDL NGALDPVPVK ISGGTPPLTV LVNGAPIDLR
RPSQAVFFAP DGPGFTRVTV TDAAGHADSV LVRLQ
//
