ID A0A6P3HIQ1_BISBB Unreviewed; 547 AA.
AC A0A6P3HIQ1;
DT 02-DEC-2020, integrated into UniProtKB/TrEMBL.
DT 02-DEC-2020, sequence version 1.
DT 28-JAN-2026, entry version 23.
DE RecName: Full=tRNA (adenine(58)-N(1))-methyltransferase {ECO:0000256|ARBA:ARBA00012796};
DE EC=2.1.1.220 {ECO:0000256|ARBA:ARBA00012796};
GN Name=TRMT61B {ECO:0000313|RefSeq:XP_010839004.1};
OS Bison bison bison (North American plains bison).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bison.
OX NCBI_TaxID=43346 {ECO:0000313|Proteomes:UP000515208, ECO:0000313|RefSeq:XP_010839004.1};
RN [1] {ECO:0000313|RefSeq:XP_010839004.1}
RP IDENTIFICATION.
RC TISSUE=Blood {ECO:0000313|RefSeq:XP_010839004.1};
RG RefSeq;
RL Submitted (AUG-2025) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an adenosine in mRNA + S-adenosyl-L-methionine = an N(1)-
CC methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+);
CC Xref=Rhea:RHEA:55392, Rhea:RHEA-COMP:12414, Rhea:RHEA-COMP:12415,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74411, ChEBI:CHEBI:74491;
CC Evidence={ECO:0000256|ARBA:ARBA00048481};
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DR RefSeq; XP_010839004.1; XM_010840702.1.
DR AlphaFoldDB; A0A6P3HIQ1; -.
DR GeneID; 104989135; -.
DR KEGG; bbis:104989135; -.
DR CTD; 55006; -.
DR OrthoDB; 5585464at2759; -.
DR Proteomes; UP000515208; Unplaced.
DR GO; GO:0005739; C:mitochondrion; IEA:TreeGrafter.
DR GO; GO:0031515; C:tRNA (m1A) methyltransferase complex; IEA:InterPro.
DR GO; GO:0160107; F:tRNA (adenine(58)-N1)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030488; P:tRNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR FunFam; 3.10.330.20:FF:000003; tRNA (Adenine(58)-N(1))-methyltransferase, mitochondrial isoform X1; 1.
DR FunFam; 3.40.50.150:FF:000181; tRNA (Adenine(58)-N(1))-methyltransferase, mitochondrial isoform X4; 1.
DR Gene3D; 3.10.330.20; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR054151; TR61B_FKBP-like.
DR InterPro; IPR049470; TRM61_C.
DR InterPro; IPR014816; tRNA_MeTrfase_Gcd14.
DR PANTHER; PTHR12133; TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR12133:SF1; TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR Pfam; PF08704; GCD14; 1.
DR Pfam; PF21985; TR61B_FKBP-like; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51620; SAM_TRM61; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000515208};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 205..258
FT /note="TR61B FKBP-like"
FT /evidence="ECO:0000259|Pfam:PF21985"
FT DOMAIN 308..537
FT /note="tRNA (adenine(58)-N(1))-methyltransferase catalytic
FT subunit TRM61 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08704"
FT REGION 96..178
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 96..111
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 151..164
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 547 AA; 61216 MW; FFDD14485B2B1A52 CRC64;
MKRLEAEQPY SILLSSRGRL RHCYAQREAE PRRAFRHSPA TAPARIVMLS TRCVGLVLLP
LRLLQRFPGF RTCLFLRRLG RESFERGRLL CFKASPRDTG NEERESEEAQ RKAPGADSPP
SVPSRVRGLG TRCLSSLESL GLPTPREESP PQESEVSSGE QGQSDPAHPG SRSPSVPALV
QPATKDEILH VSSSWSASRV VPFRVGELIL AETGKRETQF KKLFRLSNAG HLNSNWGTVP
FSEIVGKFPG QILRSSSGKH FMLRRPALED YVLLMQRGPA ITYPKFAGSA FKRMLNSWGD
ISGDILSIIL NEDINMILSM MNIHPGDTVL EAGSGSGGMS LFLSKAVGSQ GRVISFEIRK
DHHKLAMKNY RHWRDSWKIS HVEEWPDNVD FIHKDILGAT EDIQSLTFDA VALDMLNPQV
ALPVLFPNLK QGGICAVYLA NITQVIELLD GIRICELALS CEKISEVIVR DWLVCLARQK
NGILAQKVEP KINTDLQAHS QEKIRIEEES HSDFPYGSFP YIARPIHWQI GHTAFLVKLR
KCKPQLN
//
