ID A0A6P3XYT8_DINQU Unreviewed; 474 AA.
AC A0A6P3XYT8;
DT 02-DEC-2020, integrated into UniProtKB/TrEMBL.
DT 02-DEC-2020, sequence version 1.
DT 10-JUN-2026, entry version 23.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Name=LOC106748857 {ECO:0000313|RefSeq:XP_014483247.1};
OS Dinoponera quadriceps (South American ant).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Ponerinae; Ponerini; Dinoponera.
OX NCBI_TaxID=609295 {ECO:0000313|Proteomes:UP000515204, ECO:0000313|RefSeq:XP_014483247.1};
RN [1] {ECO:0000313|RefSeq:XP_014483247.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (JAN-2026) to UniProtKB.
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
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DR RefSeq; XP_014483247.1; XM_014627761.1.
DR AlphaFoldDB; A0A6P3XYT8; -.
DR GeneID; 106748857; -.
DR KEGG; dqu:106748857; -.
DR CTD; 348180; -.
DR OrthoDB; 25129at2759; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000515204; Unplaced.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000515204};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 474 AA; 53346 MW; 161E560A365B3130 CRC64;
MCSLNGYRCN SNDDEIPKNT KDSLIDIKVC RKCRIKDVDV LLLGQNGYCN MCFLTATNHK
FRAALGKSKI IRHGDSILVD HSGELNSTVL LHLIKTGMNE STHKKLIFKT TVLYIDDALP
NEITDEDRNS LQCKIAEQTK NFGFAYYIVS LSQVLNKDDT LDIRQFDNQL VKDNNDEQLH
EILTNLPDNT SRTDLLEQLR RKLLLSAARK LNCNKVFVAD STMDIATKVL GNICLGRGAQ
LSTLATFCDT RCTDIKILKP MRDFTQQEMM YYSQCHSLNP IKSKKINVTT QPATSIQALT
YNFIMGMESQ FSSTISTIMR TAEKVSARNN KDNQDMENCV LCDASLDSVS SENNVTAMKA
IEVSSLVSSK YFNARTSVTD KENESFSDKK SDRTSQCLSN NQKCCNNNSS QCGCMNGKMG
QLTTEDVQKC LCYSCQLIFR NSNLLSRLPA SLLFAVQQRL ALKKMKEDIS DFLI
//
