ID A0A6P6X5S9_COFAR Unreviewed; 3026 AA.
AC A0A6P6X5S9;
DT 02-DEC-2020, integrated into UniProtKB/TrEMBL.
DT 08-OCT-2025, sequence version 2.
DT 28-JAN-2026, entry version 26.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513, ECO:0000256|RuleBase:RU365027};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513, ECO:0000256|RuleBase:RU365027};
GN Name=LOC113739908 {ECO:0000313|RefSeq:XP_027123123.2};
OS Coffea arabica (Arabian coffee).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Gentianales; Rubiaceae; Ixoroideae; Gardenieae complex;
OC Bertiereae - Coffeeae clade; Coffeeae; Coffea.
OX NCBI_TaxID=13443 {ECO:0000313|Proteomes:UP001652660, ECO:0000313|RefSeq:XP_027123123.2};
RN [1] {ECO:0000313|Proteomes:UP001652660}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=40002058; DOI=10.3390/foods14040614;
RG RefSeq;
RA Tenea G.N., Cifuentes V., Reyes P., Cevallos-Vallejos M.;
RT "Unveiling the Microbial Signatures of Arabica Coffee Cherries: Insights
RT into Ripeness Specific Diversity, Functional Traits, and Implications for
RT Quality and Safety.";
RL Foods 14:614-614(2025).
RN [2] {ECO:0000313|RefSeq:XP_027123123.2}
RP IDENTIFICATION.
RC TISSUE=Leaves {ECO:0000313|RefSeq:XP_027123123.2};
RG RefSeq;
RL Submitted (AUG-2025) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] +
CC ADP + H(+); Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00047899,
CC ECO:0000256|RuleBase:RU365027};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU365027}.
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. ATM subfamily.
CC {ECO:0000256|ARBA:ARBA00010769, ECO:0000256|RuleBase:RU365027}.
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DR RefSeq; XP_027123123.2; XM_027267322.2.
DR GeneID; 113739908; -.
DR Proteomes; UP001652660; Chromosome 4e.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR CDD; cd05171; PIKKc_ATM; 1.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR Gene3D; 3.30.1010.10; Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR038980; ATM_plant.
DR InterPro; IPR057445; ATM_TPR.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR044107; PIKKc_ATM.
DR InterPro; IPR021668; TAN.
DR PANTHER; PTHR37079; SERINE/THREONINE-PROTEIN KINASE ATM; 1.
DR PANTHER; PTHR37079:SF4; SERINE_THREONINE-PROTEIN KINASE ATM; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR Pfam; PF11640; TAN; 1.
DR Pfam; PF25360; TPR_ATM; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU365027};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU365027};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|RuleBase:RU365027};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU365027};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU365027};
KW Reference proteome {ECO:0000313|Proteomes:UP001652660};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527,
KW ECO:0000256|RuleBase:RU365027};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365027}.
FT DOMAIN 1897..2560
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 2666..2981
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 2994..3026
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
FT REGION 1843..1867
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2405..2450
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1856..1867
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3026 AA; 344656 MW; 08D752FE9CE48DB6 CRC64;
MTAKVTSGDV QEIVSKLSSD KAKAREDGIK LLNTWLEGER SILFCKYISE KSALLKPDEL
PHSETWPFII SLLTQCVTLE IQSSKKRLPK LGFAKTLRIV VQRAEDPKFS GKNLLLVPVV
KLLFNHIWDV LRDVPSFQSE YGVILRHLLQ VQHYRLHIRK RVYSGLVLLY MEKVETSLDV
KTSGYSNPKE EVFRCILTLH SLLENPPGDF PADLREDIVK SFIGIFCHIR EEGKISRKLI
ECLNTYLLKD GPNLDSQNLE IHDAVQQFVF RCWLTTHDRS LKDALVLYAR LQLNLTRGTG
DGNALLEQLK EVLSKELDQM TTSSSNLPWN DITRDEKCGI LTRSQQRFLE LAAVVFWRAC
ANTSKPASAE KRARREHMVV QVKDGLMNGK WSWHAAFCFL IRNYSNRIRK DLIIYWFESI
CASFDRIIND ANVEHTYDGL LWTLRSLQRL SSLVLFPSSR GDLSPKSSFF MNEFDKFWHT
IWSCTVRGLP IFSNIISVAE AALVLLRNMI LSDKLITFTV PRDIWDLRLF THLPSVSVLC
FVSCYFSSRA SQGDLHEALH VRQNLLKAVL ALLNWKESSM LNDQLVVMLP PAVYALCAGC
APLSHSVPEA GMNWVMAEEQ EQENAHEFFE LSVEVLASIS HESTPQDCLP ECYQNVHLPM
KLRDLLCHEV EICTLEALKE IEIKKMLLSD IFLTCALLSN LIYCSCSMRN RDEKSYFLTK
LGEYMIGLLD QSASILERTH NDIRCGCAGS SPVFNSMDSI VASFNSFVSS PFFSKWRDKN
DIDVVIYTGI VESIERLLKG LAKLYDACSD NGKDVYSGVD LPDDFDSKML QNDPADNSKS
IIVDMELDVN SDSKDMDILN LGGKVTHGFG FSSENLKMDV LSFISSFFSV LPSLTWDTLF
DLIEKDGNQR VLENLVSCLC QHPHWSSHRR VSELIMSLNN VVDMQANLKL QCIRTLDAIC
SLVRNILLAR TAKDKNVSSL WDRISEEELI SLGDLINKVD EKNLVDWCGR TKLIGCICDF
VLLKPEIGQS MIEKLLGMLR DSDYRVRFCL AQRIGVLFQT WDGHNELFQD ICSNFGPRLV
IFSKDRVVTA KEVLAAGPQA GSLLETTITT LLHLAVESEK IELEAVFMMS VVAAIDPSQR
ELVTAVFDNL SRELNYTSRA KYMEELIGPL LFCWVACGVS LVALVEIRDM FVSSVEPCNF
ILYCCQWLLP ALLLNEDFSN LNWVAKIACQ PLATLVKIHF VHVFSVCMAL HCSKKTGWQK
GSAVLESLIL RIAEISENER DKLIKRHMVS IVNHLFSLAA ASADPAPPLF SRDTIVHAIQ
TVVDGFLEME DRSRSGGLVD KINIFRPDRV FSFIVEMHYK VTAAAHQRHK CHRLGGIEVL
VNVLGHRAGV SSSFIYLLNL VGQCIGRDAL LNQCCRIIST LLKIYKDCPS DETTRVLGEQ
LQFLVSKLVL CYSPSDNDGE NPVACSSEVL LLLRELILHS DASLYEYIKE LEPLPDLDMF
SDIRKFHEEL CLDYSAKNHL VNLAKRSLYL PPRVLVWSLQ ALHKRFFEEE ARQGWKRATL
GFLNEYWHSD QDIVSAIWHL VRMCSLDDTD NFATLVSDFV SRVGIDDPHR VVLHLPGEIS
HAPRFEEDKA EKGTDRLVQL DTVVSDELLN SLIRLLKKYL MDDSVKIIDM TSQALRGILS
TEKGQRALLL FDQHEKSLIE VHSKGVNTEL AQKLVSDLEA KFNAEAISLE NSAMWKTHDK
TFDMWVCPLV YGLIGYCDDI IIRFCQDICL VKSEVAELLF PSIMVNLAGR KDLDVDLCKL
ISFQVQENIF IESNTLMKSI KVMLDALNEL RLCHMLERST SLPGSYKREN SKHTKASSYG
SKSRSTSVKL KDNSSSRLVT TTLSWEKVYW LPIDYLVVSK SAINCGSYFT AVLYVEHWCE
HHFDSLTLGS PDFSHLEILP SHIEILVSAV TQINEPDSLY GIIQSHKLTS QITMFEHEGN
WTKALEHYDL LVQSGLKEPI SGHPKSLSYD ESQPVVPALL SKSKDQIMQK RPYKGLIRSL
QQIGCTHVLD IYCQGLASLK GQLLHDLEFT ELQYEAAWRA GNWDFSLLNG VATPVLDQKI
KCGHFNENLH SCLRALEEGE FDEFHTKLRD SKKELLLSIC HASKESTEYI YSAMVKLQIF
YHVGMAWGLR WSMLCKKTVA SSVTLRLSSE PIVPSMDQLS WLNKDWRRIL EQAQLHMNLL
EPFIAFRRVL LEILNCRDFK LQHLLEAAST LRKGIQFSQA AASLHEFKSL CTAMGKEHSN
VYWHGRIEEA KLLRAQGQHE MAINLAKYIA QDSQFDDYTS DVFRLVGKWL AETRSSNSRT
VLDKYLKHAV SLADNLKATD TKSIAKQCQM HFHLSHYADA LYRTNEERLN SNEWQAAMRL
RKFKAKELKA LIKRLKNSSK GEQTEYSLKI QELQKQLAMD GEEEEKLQED RDNFLTTALE
GYKHCLVIGD EYDVRVVFRL VSLWFGLSSR PIVINGMIST IKEVQSHKFI PLAYQIASRM
GSTKDQGPHS FQFALVSLVK KMAIEHPYHT IFQLLALANG DRIKDKQRSR NSFVVDMDKK
VAAENLLIEL HSYHGDVIRQ MKQMVEIYIK LAEMETKRED TNRKVTLPRD IRSVRELELV
PVITSNFPVD RSCQYCEGSF PHFKGLTDSV TVMNGINAPK AVECLGSDGK MYRQLAKSGN
DDLRQDAVME QFFSLVNTFL QNHRETWKRR LRISTYKVVP FTPSAGVIEW VTGTVPLGDY
LTGRSSTRNG GAHGRYGQGD WTFLKCRHHM TTESDKRRAF QEVCENFRPV MHYFFLERFL
HPANWFEKRL AYTRSVAASS MVGYIVGLGD RHSMNILIDQ ATAEVVHIDL GVAFEQGLML
KTPERVPFRL TRDMIDGMGV TGVEGVFRRC CEETLSVMRA NKEALLTIVE VFIHDPLYKW
ALSPLKALQR QKETDYDLET SLEGSEEDEY EGNKDAARAL LRVKQKLDGY EEGEMRSVRG
QVQQLVQDAI DPDRLSQMFP GWGAWM
//
