ID A0A7D5GBB1_9EURY Unreviewed; 390 AA.
AC A0A7D5GBB1;
DT 02-DEC-2020, integrated into UniProtKB/TrEMBL.
DT 02-DEC-2020, sequence version 1.
DT 18-JUN-2025, entry version 15.
DE RecName: Full=glucarate dehydratase {ECO:0000256|ARBA:ARBA00011973};
DE EC=4.2.1.40 {ECO:0000256|ARBA:ARBA00011973};
GN ORFNames=HUG10_06910 {ECO:0000313|EMBL:QLG27292.1};
OS Halorarum halophilum.
OC Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
OC Halobacteria; Halobacteriales; Haloferacaceae; Halorarum.
OX NCBI_TaxID=2743090 {ECO:0000313|EMBL:QLG27292.1, ECO:0000313|Proteomes:UP000509750};
RN [1] {ECO:0000313|EMBL:QLG27292.1, ECO:0000313|Proteomes:UP000509750}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Gai3-2 {ECO:0000313|EMBL:QLG27292.1,
RC ECO:0000313|Proteomes:UP000509750};
RA Cui H., Shi X.;
RT "Gai3-2, isolated from salt lake.";
RL Submitted (JUL-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O;
CC Xref=Rhea:RHEA:14573, ChEBI:CHEBI:15377, ChEBI:CHEBI:30612,
CC ChEBI:CHEBI:42819; EC=4.2.1.40;
CC Evidence={ECO:0000256|ARBA:ARBA00001426};
CC -!- PATHWAY: Carbohydrate acid metabolism; D-glucarate degradation; 2,5-
CC dioxopentanoate from D-glucarate: step 1/2.
CC {ECO:0000256|ARBA:ARBA00005183}.
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DR EMBL; CP058529; QLG27292.1; -; Genomic_DNA.
DR RefSeq; WP_179168867.1; NZ_CP058529.1.
DR AlphaFoldDB; A0A7D5GBB1; -.
DR GeneID; 56028549; -.
DR KEGG; halg:HUG10_06910; -.
DR OrthoDB; 42605at2157; -.
DR Proteomes; UP000509750; Chromosome.
DR Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR InterPro; IPR034593; DgoD-like.
DR InterPro; IPR036849; Enolase-like_C_sf.
DR InterPro; IPR029017; Enolase-like_N.
DR InterPro; IPR029065; Enolase_C-like.
DR InterPro; IPR013342; Mandelate_racemase_C.
DR InterPro; IPR013341; Mandelate_racemase_N_dom.
DR PANTHER; PTHR48080; D-GALACTONATE DEHYDRATASE-RELATED; 1.
DR PANTHER; PTHR48080:SF4; GLUCARATE DEHYDRATASE; 1.
DR Pfam; PF13378; MR_MLE_C; 1.
DR Pfam; PF02746; MR_MLE_N; 1.
DR SFLD; SFLDS00001; Enolase; 1.
DR SMART; SM00922; MR_MLE; 1.
DR SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE 4: Predicted;
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Reference proteome {ECO:0000313|Proteomes:UP000509750}.
FT DOMAIN 149..248
FT /note="Mandelate racemase/muconate lactonizing enzyme C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00922"
SQ SEQUENCE 390 AA; 42767 MW; 7278C57F2772B69B CRC64;
MAMRISELEI HEFTYRLDDV GMSHGHQGYD PGNVLEPPGF VLTIRTADGL EGHYRGFYFV
PPMLAQIRMA SSALLDRDPL EREDIWQDLW KAMRHLDHVG MGPIDVALWD LAGNHYGASV
SELLGGTSEP VPAYASTFMA DDNGGLDSPE AYADFAEECL DRGFPAYKLH GFGAPDGDVA
ACRAVADRVG DEMDLMLDPA SEYDTYADTL RVGRALDELD FFWYEDPMAD GGESMEMTRQ
LARDLDTPIL GVEHVRGGPY TRANHLADEA LDLVRADAHL DGGITGAMKI ANVAESFGRD
VELHVGGPAH LHCMSAIRNT NYYEHGLVHP AGIEWMSAQG FLESPEHIDD DGTVPIPDGP
GLGVEVDWDF VDERLTNHEV IDEPLVSGLA
//
