ID A0A7J6DFG7_9TELE Unreviewed; 604 AA.
AC A0A7J6DFG7;
DT 07-APR-2021, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 1.
DT 28-JAN-2026, entry version 17.
DE RecName: Full=procollagen galactosyltransferase {ECO:0000256|ARBA:ARBA00038926};
DE EC=2.4.1.50 {ECO:0000256|ARBA:ARBA00038926};
GN ORFNames=G5714_000127 {ECO:0000313|EMBL:KAF4118076.1};
OS Onychostoma macrolepis.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Cyprinidae; Acrossocheilinae; Onychostoma.
OX NCBI_TaxID=369639 {ECO:0000313|EMBL:KAF4118076.1, ECO:0000313|Proteomes:UP000579812};
RN [1] {ECO:0000313|EMBL:KAF4118076.1, ECO:0000313|Proteomes:UP000579812}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SWU-2019 {ECO:0000313|EMBL:KAF4118076.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:KAF4118076.1};
RA Wang D.;
RT "Chromosome-level genome assembly of a cyprinid fish Onychostoma macrolepis
RT by integration of Nanopore Sequencing, Bionano and Hi-C technology.";
RL Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5R)-5-hydroxy-L-lysyl-[collagen] + UDP-alpha-D-galactose =
CC (5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysyl-[collagen] + UDP +
CC H(+); Xref=Rhea:RHEA:12637, Rhea:RHEA-COMP:12752, Rhea:RHEA-
CC COMP:12753, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914,
CC ChEBI:CHEBI:133442, ChEBI:CHEBI:133443; EC=2.4.1.50;
CC Evidence={ECO:0000256|ARBA:ARBA00048515};
CC -!- SIMILARITY: Belongs to the glycosyltransferase 25 family.
CC {ECO:0000256|ARBA:ARBA00006721}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF4118076.1}.
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DR EMBL; JAAMOB010000001; KAF4118076.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A7J6DFG7; -.
DR OrthoDB; 47375at2759; -.
DR Proteomes; UP000579812; Unassembled WGS sequence.
DR GO; GO:0050211; F:procollagen galactosyltransferase activity; IEA:UniProtKB-EC.
DR CDD; cd06532; Glyco_transf_25; 1.
DR FunFam; 3.90.550.10:FF:000048; Glycosyltransferase 25 family member 1; 1.
DR Gene3D; 3.90.550.10; Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A; 1.
DR InterPro; IPR050757; Collagen_mod_GT25.
DR InterPro; IPR002654; Glyco_trans_25.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR10730:SF28; PROCOLLAGEN GALACTOSYLTRANSFERASE 1; 1.
DR PANTHER; PTHR10730; PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER; 1.
DR Pfam; PF13704; Glyco_tranf_2_4; 1.
DR Pfam; PF01755; Glyco_transf_25; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Reference proteome {ECO:0000313|Proteomes:UP000579812};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT SIGNAL 1..18
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 19..604
FT /note="procollagen galactosyltransferase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5029553729"
FT DOMAIN 323..506
FT /note="Glycosyl transferase family 25"
FT /evidence="ECO:0000259|Pfam:PF01755"
FT REGION 569..604
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 569..580
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 584..597
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 604 AA; 70923 MW; 406BB62516179C36 CRC64;
MHLLCVFVLL LWTGPARGYF PEERWSPESA LLAPRVLLAL VCRNSAHSLP HVLGAIDRLN
YPKDRMAVWV ATDHNSDNTT AILREWLVNV QNFYHYVEWR PQEEPSVYED ESGPKHWTNL
RYEHLMKLRQ AALDTAREMW ADYFMMVDCD NLLTNRDVLW KLMQENKTMV APMLESRAAY
SNFWCGMTSQ GYYKRTPAYM PIRRQERKGC FAVPMVHSTF LLDLRKEASR ELAFFPPHPD
YSWAFDDIII FAFSARMAEV QMYICNRETY GYFPVPLRSH NTLQDEEDSF LHSLLEVMVR
NPPTEPSVYL SLPPKQLDKM GFDEVFMINL QRRSDRRERM LRTLYEQEIE CKIIAAVDGK
ALNVSQIEAL GIHMMPGYSD PYHGRPLTKG ELGCFLSHFN IWTEIVDRGL QTSLVIEDDL
RFEIFFKRRL QNLMHEIESQ RLDWDLIYIG RKRMQVDRSE KSVPRIHNLV EADYSYWTLG
YAISLRGAQK LLRAEPLKNM LPVDEFLPVM YNKHPIEDYM SHFDRRDLRA FSAEPLLVYP
THYTGDAGYI SDTETSTVWD NETVRTDWDR ARSRKSREQE ELSSEAQNSD VLQSPLDSTA
RDEL
//
