ID A0A7J7CDY2_TRIWF Unreviewed; 283 AA.
AC A0A7J7CDY2;
DT 07-APR-2021, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 1.
DT 18-JUN-2025, entry version 17.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN ORFNames=HS088_TW18G00776 {ECO:0000313|EMBL:KAF5732087.1};
OS Tripterygium wilfordii (Thunder God vine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Celastrales; Celastraceae; Tripterygium.
OX NCBI_TaxID=458696 {ECO:0000313|EMBL:KAF5732087.1, ECO:0000313|Proteomes:UP000593562};
RN [1] {ECO:0000313|EMBL:KAF5732087.1, ECO:0000313|Proteomes:UP000593562}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. XIE 37 {ECO:0000313|Proteomes:UP000593562};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF5732087.1};
RX PubMed=32080175; DOI=10.1038/s41467-020-14776-1;
RA Tu L., Su P., Zhang Z., Gao L., Wang J., Hu T., Zhou J., Zhang Y., Zhao Y.,
RA Liu Y., Song Y., Tong Y., Lu Y., Yang J., Xu C., Jia M., Peters R.J.,
RA Huang L., Gao W.;
RT "Genome of Tripterygium wilfordii and identification of cytochrome P450
RT involved in triptolide biosynthesis.";
RL Nat. Commun. 11:971-971(2020).
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF5732087.1}.
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DR EMBL; JAAARO010000018; KAF5732087.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A7J7CDY2; -.
DR FunCoup; A0A7J7CDY2; 344.
DR InParanoid; A0A7J7CDY2; -.
DR OrthoDB; 10009520at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000593562; Chromosome 18.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd22582; BRcat_RBR_unk; 1.
DR CDD; cd22584; Rcat_RBR_unk; 1.
DR FunFam; 3.30.40.10:FF:000230; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 2.
DR SMART; SM00647; IBR; 2.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000593562};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 81..283
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 85..130
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 54..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..65
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 283 AA; 31997 MW; C4180FA9A6ADBB54 CRC64;
MTGRSRRSRD NKTRFLDDFV IPVLSDEKEK IPMSDEEYAL QLQLQEALIS SSVSSSRGSI
KGPSKQPIHP RKKRKGTVES SQSFCTICLD VKLHGEMFVS HSCTHTFCTD CISQYVATKI
QENISMVKCP DLNCKGILEP QNCREIVPAE VFDRWENTLC ESLVLGSQKF YCPFGDCSAL
LVDDGGVTVT SSECPNCRRL FCAQCKVAWH AGMTCSEFEK FKGGESKKED KMVMKLVKKK
KWKRCPNCSF YVDKISGCIH ISCRCGFEFC YRCGSKWSDD HAC
//
