ID A0A7J7CER3_TRIWF Unreviewed; 335 AA.
AC A0A7J7CER3;
DT 07-APR-2021, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 1.
DT 08-OCT-2025, entry version 17.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=HS088_TW18G01120 {ECO:0000313|EMBL:KAF5732425.1};
OS Tripterygium wilfordii (Thunder God vine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Celastrales; Celastraceae; Tripterygium.
OX NCBI_TaxID=458696 {ECO:0000313|EMBL:KAF5732425.1, ECO:0000313|Proteomes:UP000593562};
RN [1] {ECO:0000313|EMBL:KAF5732425.1, ECO:0000313|Proteomes:UP000593562}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. XIE 37 {ECO:0000313|Proteomes:UP000593562};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF5732425.1};
RX PubMed=32080175; DOI=10.1038/s41467-020-14776-1;
RA Tu L., Su P., Zhang Z., Gao L., Wang J., Hu T., Zhou J., Zhang Y., Zhao Y.,
RA Liu Y., Song Y., Tong Y., Lu Y., Yang J., Xu C., Jia M., Peters R.J.,
RA Huang L., Gao W.;
RT "Genome of Tripterygium wilfordii and identification of cytochrome P450
RT involved in triptolide biosynthesis.";
RL Nat. Commun. 11:971-971(2020).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the SINA (Seven in absentia) family.
CC {ECO:0000256|ARBA:ARBA00009119}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF5732425.1}.
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DR EMBL; JAAARO010000018; KAF5732425.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A7J7CER3; -.
DR FunCoup; A0A7J7CER3; 1160.
DR InParanoid; A0A7J7CER3; -.
DR OrthoDB; 941555at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000593562; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR CDD; cd16571; RING-HC_SIAHs; 1.
DR CDD; cd03829; Sina; 1.
DR FunFam; 3.30.40.10:FF:000041; E3 ubiquitin-protein ligase SINAT3; 1.
DR FunFam; 3.30.40.10:FF:000483; E3 ubiquitin-protein ligase SINAT3; 1.
DR FunFam; 2.60.210.10:FF:000004; E3 ubiquitin-protein ligase SINAT5-like; 1.
DR Gene3D; 2.60.210.10; Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 2.
DR InterPro; IPR018121; 7-in-absentia-prot_TRAF-dom.
DR InterPro; IPR052088; E3_ubiquitin-ligase_SINA.
DR InterPro; IPR049548; Sina-like_RING.
DR InterPro; IPR008974; TRAF-like.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR013010; Znf_SIAH.
DR PANTHER; PTHR10315; E3 UBIQUITIN PROTEIN LIGASE SIAH; 1.
DR PANTHER; PTHR10315:SF80; E3 UBIQUITIN-PROTEIN LIGASE SINAT3; 1.
DR Pfam; PF21362; Sina_RING; 1.
DR Pfam; PF03145; Sina_TRAF; 1.
DR Pfam; PF21361; Sina_ZnF; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR SUPFAM; SSF49599; TRAF domain-like; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
DR PROSITE; PS51081; ZF_SIAH; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000593562};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00455}.
FT DOMAIN 73..109
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT DOMAIN 126..186
FT /note="SIAH-type"
FT /evidence="ECO:0000259|PROSITE:PS51081"
SQ SEQUENCE 335 AA; 37915 MW; 1765682C52BB27AE CRC64;
MELDTVECLS SSDGFDEDGI HHHSLHHPSH PHLHNLHQFS SSKLHNANGG NNGNILGPTA
IAPATSVHEL LECPVCTNSM YPPIHQCHNG HTLCSTCKTR VQNRCPTCRQ ELGDIRCLAL
EKVAESLDLP CKFYSLGCQE IFPYYSKLKH ETICNFRPYN CPYAGSECSV TGDIPFLVTH
LRDDHKVDMH TGCTFNHRYV KSNPREVENA TWMLTVFHCF GQYFCLHFEA FQLGTAPVYM
AFLRFMGDEA EARNYSYSLE VGANGRKQIW EGTPRSVRDS HRKVRDSHDG LIIQRNMALF
FSGGDRKELK LRVTGRIWKE QQNSDGGVCI PNLCS
//
