ID A0A7J7DXT5_TRIWF Unreviewed; 548 AA.
AC A0A7J7DXT5;
DT 07-APR-2021, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 1.
DT 18-JUN-2025, entry version 17.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN ORFNames=HS088_TW02G00137 {ECO:0000313|EMBL:KAF5751127.1};
OS Tripterygium wilfordii (Thunder God vine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Celastrales; Celastraceae; Tripterygium.
OX NCBI_TaxID=458696 {ECO:0000313|EMBL:KAF5751127.1, ECO:0000313|Proteomes:UP000593562};
RN [1] {ECO:0000313|EMBL:KAF5751127.1, ECO:0000313|Proteomes:UP000593562}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. XIE 37 {ECO:0000313|Proteomes:UP000593562};
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF5751127.1};
RX PubMed=32080175; DOI=10.1038/s41467-020-14776-1;
RA Tu L., Su P., Zhang Z., Gao L., Wang J., Hu T., Zhou J., Zhang Y., Zhao Y.,
RA Liu Y., Song Y., Tong Y., Lu Y., Yang J., Xu C., Jia M., Peters R.J.,
RA Huang L., Gao W.;
RT "Genome of Tripterygium wilfordii and identification of cytochrome P450
RT involved in triptolide biosynthesis.";
RL Nat. Commun. 11:971-971(2020).
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF5751127.1}.
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DR EMBL; JAAARO010000002; KAF5751127.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A7J7DXT5; -.
DR FunCoup; A0A7J7DXT5; 4885.
DR InParanoid; A0A7J7DXT5; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000593562; Chromosome 2.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd20346; BRcat_RBR_ANKIB1; 1.
DR CDD; cd22586; Rcat_RBR_ARI1-like; 1.
DR CDD; cd16773; RING-HC_RBR_TRIAD1; 1.
DR FunFam; 1.20.120.1750:FF:000013; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000019; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR045840; Ariadne.
DR InterPro; IPR048962; ARIH1-like_UBL.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF19422; Ariadne; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF22191; IBR_1; 1.
DR Pfam; PF21235; UBA_ARI1; 1.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000593562};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 116..330
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 120..168
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 526..548
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..12
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 548 AA; 63585 MW; 62046766129170C1 CRC64;
MEDCVASDEE YYSSDRDSLD GFDNEEPDFQ WVPPRVPTTK VITRESLLAA QKEDLRRVMD
LLSLREHHAR TLLIHYRWDV ERLFAVFVDK GKTRLFTEAG VTIVEHLDIQ SPLYSSTATC
DICMDDVSGD KVTRMDCGHC FCNECWTGHF IVKINEGQSK RIRCMAHKCN SICDEAIVRN
LVGKEHPDLA EKFERFLLES YIEDNKMVKW CPSAPHCGNA IRVEDDESFE VECSCGLQFC
FGCISEAHSP CSCLMWELWV KKCRDESETV NWMTVHTKPC PKCHKPVEKN GGCNLVSCIC
GQSFCWLCGG ATGQDHTWSR IAGHSCGRYK EDQQKQTERA KRDLYRYMHY HNRYKAHTDS
FKLENKLRET ILEKVSISEE RESKLKDFSW VNNGLYRLFR SRRVLSYSYP FAYYMFGEEL
FADEMTKEER EIKQNLFEDQ QQQLEANVEK LSKFLEEPFD QYPDDKVMEI RMQVINLSAV
VDLLCKKMYE CIENDLLGPL QLGIHNIAPY KSKGIERASE LSSCWSTKPN NTDKYPPSDF
VTSGHSRG
//
