ID A0A7J8MD02_9ROSI Unreviewed; 449 AA.
AC A0A7J8MD02;
DT 07-APR-2021, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 1.
DT 28-JAN-2026, entry version 16.
DE RecName: Full=U-box domain-containing protein {ECO:0000256|RuleBase:RU369093, ECO:0000259|PROSITE:PS51698};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU369093};
DE AltName: Full=RING-type E3 ubiquitin transferase PUB {ECO:0000256|RuleBase:RU369093};
GN ORFNames=Golob_007633 {ECO:0000313|EMBL:MBA0562601.1};
OS Gossypium lobatum.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=34289 {ECO:0000313|EMBL:MBA0562601.1, ECO:0000313|Proteomes:UP000593572};
RN [1] {ECO:0000313|EMBL:MBA0562601.1, ECO:0000313|Proteomes:UP000593572}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=157 {ECO:0000313|EMBL:MBA0562601.1};
RC TISSUE=Leaf {ECO:0000313|EMBL:MBA0562601.1};
RX PubMed=30476109;
RA Grover C.E., Arick M.A. 2nd, Thrash A., Conover J.L., Sanders W.S.,
RA Peterson D.G., Frelichowski J.E., Scheffler J.A., Scheffler B.E.,
RA Wendel J.F.;
RT "Insights into the evolution of the New World diploid cottons (Gossypium,
RT subgenus Houzingenia) based on genome sequencing.";
RL Genome Biol. Evol. 11:53-71(2019).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC {ECO:0000256|RuleBase:RU369093}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU369093};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369093}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:MBA0562601.1}.
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DR EMBL; JABEZX010000008; MBA0562601.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A7J8MD02; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000593572; Unassembled WGS sequence.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 2.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR058678; ARM_PUB.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849:SF142; U-BOX DOMAIN-CONTAINING PROTEIN 31; 1.
DR PANTHER; PTHR22849; WDSAM1 PROTEIN; 1.
DR Pfam; PF25598; ARM_PUB; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000593572};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU369093};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|RuleBase:RU369093}.
FT DOMAIN 64..138
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
SQ SEQUENCE 449 AA; 49111 MW; 70AADA3D756A6C66 CRC64;
MFQSSKRDGV VVAAFDGSGD GQVLDLDTAV KDGVLGGVGG GVIGAAVGEK LDLKKMIEEL
DLPEIPSVFI CPISLEPMQD PVTLCTGQTY ERSNILKWFN LGHYTCPTTM QELWDDSVTP
NRTLYHLIYT WFSQKYLLMK KRSEDVQGRV SELLQTLKKA KGQARIQALK ELKQVVAARA
TAMKTVVDEG GVSLISSLLG PFTSHAVGSE AIAVLVNLEL DSESKTNLMQ PTKISLMVDM
LNEGTVETKI NCTRLIEKLM EEKEFRAESI SSHRLLVGLM RLVKDKRHTN GILPGLSLLR
SICLHKQVMG LIVSIGAVSQ LVELLPALEP DCLELALFIL DTLSSLPEGK AALKNCGNTI
PNMVRLLMRI SECCTQYALS ILWSVCKLAP EECSSVAVEA GLAAKLLLVI QSGCNPVLKQ
KSSELLKLCS LNYTDTIFIS KCKLTRTIL
//
