ID A0A7K6KHL4_9PASE Unreviewed; 146 AA.
AC A0A7K6KHL4;
DT 07-APR-2021, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 1.
DT 08-OCT-2025, entry version 12.
DE RecName: Full=Sterol O-acyltransferase 1 {ECO:0000256|ARBA:ARBA00040378};
DE AltName: Full=Acyl-coenzyme A:cholesterol acyltransferase 1 {ECO:0000256|ARBA:ARBA00041634};
DE AltName: Full=Cholesterol acyltransferase 1 {ECO:0000256|ARBA:ARBA00042375};
DE Flags: Fragment;
GN Name=Soat1 {ECO:0000313|EMBL:NWW11345.1};
GN ORFNames=OREARF_R10652 {ECO:0000313|EMBL:NWW11345.1};
OS Oreocharis arfaki (tit berrypecker).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Neoaves; Telluraves; Australaves;
OC Passeriformes; Passeroidea; Paramythiidae; Oreocharis.
OX NCBI_TaxID=979223 {ECO:0000313|EMBL:NWW11345.1, ECO:0000313|Proteomes:UP000542358};
RN [1] {ECO:0000313|EMBL:NWW11345.1, ECO:0000313|Proteomes:UP000542358}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B10K-DU-029-42 {ECO:0000313|EMBL:NWW11345.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:NWW11345.1};
RA Zhang G.;
RT "Bird 10,000 Genomes (B10K) Project - Family phase.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(11Z)-octadecenoyl-CoA + cholesterol = cholesteryl (11Z)-
CC octadecenoate + CoA; Xref=Rhea:RHEA:64324, ChEBI:CHEBI:16113,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:75121, ChEBI:CHEBI:88768;
CC Evidence={ECO:0000256|ARBA:ARBA00036418};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64325;
CC Evidence={ECO:0000256|ARBA:ARBA00036418};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(7Z)-octadecenoyl-CoA + cholesterol = cholesteryl (7Z)-
CC octadecenoate + CoA; Xref=Rhea:RHEA:64328, ChEBI:CHEBI:16113,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:152049, ChEBI:CHEBI:152050;
CC Evidence={ECO:0000256|ARBA:ARBA00036957};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64329;
CC Evidence={ECO:0000256|ARBA:ARBA00036957};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-hexadecenoyl-CoA + cholesterol = cholesteryl (9Z)-
CC hexadecenoate + CoA; Xref=Rhea:RHEA:64320, ChEBI:CHEBI:16113,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:61540, ChEBI:CHEBI:84323;
CC Evidence={ECO:0000256|ARBA:ARBA00037015};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64321;
CC Evidence={ECO:0000256|ARBA:ARBA00037015};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + cholesterol = cholesteryl
CC (9Z,12Z)-octadecadienoate + CoA; Xref=Rhea:RHEA:42796,
CC ChEBI:CHEBI:16113, ChEBI:CHEBI:41509, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57383; Evidence={ECO:0000256|ARBA:ARBA00036867};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42797;
CC Evidence={ECO:0000256|ARBA:ARBA00036867};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesterol + hexadecanoyl-CoA = cholesteryl hexadecanoate +
CC CoA; Xref=Rhea:RHEA:42792, ChEBI:CHEBI:3663, ChEBI:CHEBI:16113,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379;
CC Evidence={ECO:0000256|ARBA:ARBA00036161};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42793;
CC Evidence={ECO:0000256|ARBA:ARBA00036161};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=octadecanoyl-CoA + cholesterol = cholesteryl octadecanoate +
CC CoA; Xref=Rhea:RHEA:42812, ChEBI:CHEBI:16113, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57394, ChEBI:CHEBI:82750;
CC Evidence={ECO:0000256|ARBA:ARBA00048346};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42813;
CC Evidence={ECO:0000256|ARBA:ARBA00048346};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004477}.
CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC Sterol o-acyltransferase subfamily. {ECO:0000256|ARBA:ARBA00009010}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:NWW11345.1}.
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DR EMBL; VZRR01011050; NWW11345.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A7K6KHL4; -.
DR Proteomes; UP000542358; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015485; F:cholesterol binding; IEA:TreeGrafter.
DR GO; GO:0034736; F:cholesterol O-acyltransferase activity; IEA:TreeGrafter.
DR GO; GO:0000062; F:fatty-acyl-CoA binding; IEA:TreeGrafter.
DR GO; GO:0033344; P:cholesterol efflux; IEA:TreeGrafter.
DR GO; GO:0008203; P:cholesterol metabolic process; IEA:TreeGrafter.
DR InterPro; IPR004299; MBOAT_fam.
DR InterPro; IPR014371; Oat_ACAT_DAG_ARE.
DR PANTHER; PTHR10408; STEROL O-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR10408:SF6; STEROL O-ACYLTRANSFERASE 1; 1.
DR Pfam; PF03062; MBOAT; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000313|EMBL:NWW11345.1};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000542358};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:NWW11345.1};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 61..82
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 102..130
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:NWW11345.1"
FT NON_TER 146
FT /evidence="ECO:0000313|EMBL:NWW11345.1"
SQ SEQUENCE 146 AA; 17251 MW; CC79691FBEA16B91 CRC64;
QVRLVMKAHS FIRENVPRVL SSVKDKAGTV HIPRISQYLY FLFAPTLIYR DNYPRNPTIR
WGYVATKFAQ VLGSLFYAYY IFVRLCIPQF RNSSQETFNL RGLVLCIFNS ILPGVLILFL
VFFAFLHCWL NAFAEMLRFA DRMFYK
//
