ID A0A7L0LI36_9SYLV Unreviewed; 731 AA.
AC A0A7L0LI36;
DT 07-APR-2021, integrated into UniProtKB/TrEMBL.
DT 07-APR-2021, sequence version 1.
DT 28-JAN-2026, entry version 19.
DE RecName: Full=Polyribonucleotide nucleotidyltransferase 1, mitochondrial {ECO:0000256|ARBA:ARBA00072100};
DE EC=2.7.7.8 {ECO:0000256|ARBA:ARBA00012416};
DE AltName: Full=Polynucleotide phosphorylase 1 {ECO:0000256|ARBA:ARBA00031451};
DE Flags: Fragment;
GN Name=Pnpt1 {ECO:0000313|EMBL:NXK67362.1};
GN ORFNames=SYLVIR_R12945 {ECO:0000313|EMBL:NXK67362.1};
OS Sylvietta virens (Green crombec).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Neoaves; Telluraves; Australaves;
OC Passeriformes; Sylvioidea; Sylviidae; Acrocephalinae; Sylvietta.
OX NCBI_TaxID=208069 {ECO:0000313|EMBL:NXK67362.1, ECO:0000313|Proteomes:UP000567822};
RN [1] {ECO:0000313|EMBL:NXK67362.1, ECO:0000313|Proteomes:UP000567822}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B10K-DU-009-59 {ECO:0000313|EMBL:NXK67362.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:NXK67362.1};
RA Zhang G.;
RT "Bird 10,000 Genomes (B10K) Project - Family phase.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBUNIT: Homotrimer; in free form. Homooligomer. Component of the
CC mitochondrial degradosome (mtEXO) complex which is a heteropentamer
CC containing 2 copies of SUPV3L1 and 3 copies of PNPT1. As part of the
CC mitochondrial degradosome complex, interacts with GRSF1 in an RNA-
CC dependent manner; the interaction enhances the activity of the complex.
CC Interacts with TCL1A; the interaction has no effect on PNPT1
CC exonuclease activity. {ECO:0000256|ARBA:ARBA00064869}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC Mitochondrion intermembrane space {ECO:0000256|ARBA:ARBA00060410};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00060410}.
CC Mitochondrion matrix {ECO:0000256|ARBA:ARBA00004305}.
CC -!- SIMILARITY: Belongs to the polyribonucleotide nucleotidyltransferase
CC family. {ECO:0000256|ARBA:ARBA00007404}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:NXK67362.1}.
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DR EMBL; VXAN01000366; NXK67362.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A7L0LI36; -.
DR Proteomes; UP000567822; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
DR GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0000175; F:3'-5'-RNA exonuclease activity; IEA:TreeGrafter.
DR GO; GO:0004654; F:polyribonucleotide nucleotidyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000958; P:mitochondrial mRNA catabolic process; IEA:TreeGrafter.
DR GO; GO:0000965; P:mitochondrial RNA 3'-end processing; IEA:TreeGrafter.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR CDD; cd09033; KH-I_PNPT1; 1.
DR CDD; cd11363; RNase_PH_PNPase_1; 1.
DR CDD; cd11364; RNase_PH_PNPase_2; 1.
DR FunFam; 3.30.230.70:FF:000032; Polyribonucleotide nucleotidyltransferase 1; 1.
DR FunFam; 1.10.10.400:FF:000001; Polyribonucleotide nucleotidyltransferase 1, mitochondrial; 1.
DR FunFam; 2.40.50.140:FF:000113; polyribonucleotide nucleotidyltransferase 1, mitochondrial; 1.
DR FunFam; 3.30.1370.10:FF:000044; Polyribonucleotide nucleotidyltransferase 1, mitochondrial; 1.
DR FunFam; 3.30.230.70:FF:000008; polyribonucleotide nucleotidyltransferase 1, mitochondrial; 1.
DR Gene3D; 3.30.230.70; GHMP Kinase, N-terminal domain; 2.
DR Gene3D; 3.30.1370.10; K Homology domain, type 1; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 1.10.10.400; Polyribonucleotide nucleotidyltransferase, RNA-binding domain; 1.
DR InterPro; IPR001247; ExoRNase_PH_dom1.
DR InterPro; IPR015847; ExoRNase_PH_dom2.
DR InterPro; IPR036345; ExoRNase_PH_dom2_sf.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR012162; PNPase.
DR InterPro; IPR027408; PNPase/RNase_PH_dom_sf.
DR InterPro; IPR015848; PNPase_PH_RNA-bd_bac/org-type.
DR InterPro; IPR036456; PNPase_PH_RNA-bd_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR003029; S1_domain.
DR NCBIfam; TIGR03591; polynuc_phos; 1.
DR NCBIfam; NF008805; PRK11824.1; 1.
DR PANTHER; PTHR11252; POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE; 1.
DR PANTHER; PTHR11252:SF0; POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL; 1.
DR Pfam; PF03726; PNPase; 1.
DR Pfam; PF01138; RNase_PH; 2.
DR Pfam; PF03725; RNase_PH_C; 1.
DR Pfam; PF00575; S1; 1.
DR PIRSF; PIRSF005499; PNPase; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF54791; Eukaryotic type KH-domain (KH-domain type I); 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF46915; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3; 1.
DR SUPFAM; SSF55666; Ribonuclease PH domain 2-like; 2.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 2.
DR PROSITE; PS50084; KH_TYPE_1; 1.
DR PROSITE; PS50126; S1; 1.
PE 3: Inferred from homology;
KW Acetylation {ECO:0000256|ARBA:ARBA00022990};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000567822};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00117};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:NXK67362.1};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 627..698
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:NXK67362.1"
FT NON_TER 731
FT /evidence="ECO:0000313|EMBL:NXK67362.1"
SQ SEQUENCE 731 AA; 79846 MW; A35543FD35575A21 CRC64;
RKMELSSGKL AKFADGSAVV QLGDTAVMVT AVSKTKPSPS QFMPLVVDYR QKAAAAGRIP
TNYLRRELGS TDKEILTSRV IDRSIRPLFP AGYFYDTQIL CNLLAVDGVN DPDILAINGA
SAALALSDIP WNGPIGAVRV GLVDGETVIN PTRKEMASSA LNLVVAGAPQ SQVVMLEATA
ENILQQDFCH AIKVGVKQTQ QIIQGIQQLV KEGGVVKRTV QKLFIAPEEI VELAKKLASN
KIYAVFTDSS HDKISRDEAI NKIRLETEEQ LKEKFPEAEP YEIMESFNVV SKDIFRSLVL
NEYRRCDGRE LTSLRDIKCE VNMFKMLHGS ALFQRGQTQV VLCTVTFDSL ESSVKSDVIS
TAVSGIKDKN FMLHYEFPPY ATNEIGKVTG MNRRELGHGA LAERALKPVI PQDFPFTIRV
TSEVLESNGS SSMASACGGS LALMDAGVPV SNAVAGVAIG LVTKCSQGKG DIEDYRLLTD
ILGIEDYFGD MDFKMAGTNK GITALQVDLK LPGIPIKIVV EAIQQATAAK REILQIMNQT
LAKPRPNRKE NGPVVETIHV PLSKRLRFVG PGAYNLKKLQ AQTGVTVSQL DEETYSVFAP
TPGAMHEARE FIGEICKDDQ EVNLEFGAIY TATITEIRDS GVMVKLYPNM TPVLLHNSQL
DQRKIKHPTA LGLEVGQEIQ VKYFGRDPTD GRMRLSRKIL QSPATSTLKT LTDKNSIVLG
GTVPQTSTSS Q
//
