ID A0A7R7CQF6_9HYPH Unreviewed; 376 AA.
AC A0A7R7CQF6;
DT 29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 1.
DT 18-JUN-2025, entry version 11.
DE RecName: Full=Cobalt-precorrin-5B C(1)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_00787};
DE EC=2.1.1.195 {ECO:0000256|HAMAP-Rule:MF_00787};
DE AltName: Full=Cobalt-precorrin-6A synthase {ECO:0000256|HAMAP-Rule:MF_00787};
GN Name=cbiD {ECO:0000256|HAMAP-Rule:MF_00787,
GN ECO:0000313|EMBL:BCH35445.1};
GN ORFNames=MesoLjLc_73750 {ECO:0000313|EMBL:BCH35445.1};
OS Mesorhizobium sp. L-8-10.
OC Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium.
OX NCBI_TaxID=2744523 {ECO:0000313|EMBL:BCH35445.1, ECO:0000313|Proteomes:UP000595886};
RN [1] {ECO:0000313|EMBL:BCH35445.1, ECO:0000313|Proteomes:UP000595886}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=L-8-10 {ECO:0000313|EMBL:BCH35445.1,
RC ECO:0000313|Proteomes:UP000595886};
RA Bamba M., Aoki S., Kajita T., Setoguchi H., Watano Y., Sato S.,
RA Tsuchimatsu T.;
RT "Massive rhizobial genomic variation associated with partner quality in
RT Lotus-Mesorhizobium symbiosis.";
RL FEMS Microbiol. Ecol. 96:0-fiaa202(2020).
CC -!- FUNCTION: Catalyzes the methylation of C-1 in cobalt-precorrin-5B to
CC form cobalt-precorrin-6A. {ECO:0000256|HAMAP-Rule:MF_00787}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A +
CC S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:26285, ChEBI:CHEBI:57856,
CC ChEBI:CHEBI:59789, ChEBI:CHEBI:60063, ChEBI:CHEBI:60064;
CC EC=2.1.1.195; Evidence={ECO:0000256|HAMAP-Rule:MF_00787};
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route):
CC step 6/10. {ECO:0000256|HAMAP-Rule:MF_00787}.
CC -!- SIMILARITY: Belongs to the CbiD family. {ECO:0000256|HAMAP-
CC Rule:MF_00787}.
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DR EMBL; AP023263; BCH35445.1; -; Genomic_DNA.
DR RefSeq; WP_202309077.1; NZ_AP023263.1.
DR AlphaFoldDB; A0A7R7CQF6; -.
DR UniPathway; UPA00148; UER00227.
DR Proteomes; UP000595886; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019251; P:anaerobic cobalamin biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.2110.10; CbiD-like; 1.
DR HAMAP; MF_00787; CbiD; 1.
DR InterPro; IPR002748; CbiD.
DR InterPro; IPR036074; CbiD_sf.
DR NCBIfam; TIGR00312; cbiD; 1.
DR NCBIfam; NF000849; PRK00075.1-1; 1.
DR PANTHER; PTHR35863; COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR35863:SF1; COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE; 1.
DR Pfam; PF01888; CbiD; 1.
DR PIRSF; PIRSF026782; CbiD; 1.
DR SUPFAM; SSF111342; CbiD-like; 1.
PE 3: Inferred from homology;
KW Cobalamin biosynthesis {ECO:0000256|ARBA:ARBA00022573, ECO:0000256|HAMAP-
KW Rule:MF_00787}; Membrane {ECO:0000256|SAM:Phobius};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|HAMAP-
KW Rule:MF_00787}; Reference proteome {ECO:0000313|Proteomes:UP000595886};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_00787}; Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00787}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 28..376
FT /note="Cobalt-precorrin-5B C(1)-methyltransferase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5032329811"
FT TRANSMEM 163..187
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 357..376
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 376 AA; 38896 MW; 73829BED67408E00 CRC64;
MTKNERPLRR GWTTGTCAAA ASKAACAALL SGDFPDSVVV TLPGGQQPAF ALARWEKGEG
AAMAGIVKDA GDDPDVTHGA LVMSTVRRGR KGSGIVYRAG PGVGTVTRAG LPLPPGEASI
NPVPRRMIAQ AIAEIAGTDA DFEVEISVED GEEIAKKTLN GRLGILGGIS ILGTTGIVIP
FSCSAWIHSI YRGIDVARAA GLDHVAGSTG STSEAAVQKF HDLPEIALID MGDFVGGMLK
YLRSHPVRRV TIAGGVAKMT KLAQGMLDVH SKRGAADLDA LAAVALEAGA DHELAERIRG
ANMVAEAFQI AVTEGVPLGD TIAARAWATA AAVLDDPAIE LEMLVFDRNG VFLGQAPFAP
SHGAPSHGPS RPRNRL
//
