ID   A0A814C474_9BILA        Unreviewed;       421 AA.
AC   A0A814C474;
DT   29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT   29-SEP-2021, sequence version 1.
DT   08-OCT-2025, entry version 10.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN   ORFNames=OXX778_LOCUS13200 {ECO:0000313|EMBL:CAF0936675.1};
OS   Brachionus calyciflorus.
OC   Eukaryota; Metazoa; Spiralia; Gnathifera; Rotifera; Eurotatoria;
OC   Monogononta; Pseudotrocha; Ploima; Brachionidae; Brachionus.
OX   NCBI_TaxID=104777 {ECO:0000313|EMBL:CAF0936675.1, ECO:0000313|Proteomes:UP000663879};
RN   [1] {ECO:0000313|EMBL:CAF0936675.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Ploen Becks lab {ECO:0000313|EMBL:CAF0936675.1};
RA   Nowell W R.;
RL   Submitted (FEB-2021) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000256|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC       Rule:MF_03054}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CAF0936675.1}.
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DR   EMBL; CAJNOC010002494; CAF0936675.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A814C474; -.
DR   OrthoDB; 25129at2759; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000663879; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR   PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR   Pfam; PF10288; CTU2; 1.
DR   SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE   3: Inferred from homology;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW   Reference proteome {ECO:0000313|Proteomes:UP000663879};
KW   tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW   Rule:MF_03054}.
SQ   SEQUENCE   421 AA;  47945 MW;  DB4859568C23FF27 CRC64;
     MCSNNEGGLT KFEPKPDLAL FRKCLKCDKN SVVVTRINDA MCKECFVEYF THKFRATIGK
     SKLFDKDEVV MFPYSSSDSS VAMLHLLFKG VGEEAKKKFT FKTICVYIED NYVFESNSST
     HERITFMENV KKSVEYSGFK FYYSLIENCL EPSKNIVSDE IAKLNLNNEN KEKFLNLINS
     CKNQTSKEEL LKRLKTQLIN RIAIELKIKK IIFPVSCTNL TIDMLTNVAL GKGAHLSIET
     SLEEKLSNGL VYVRPLRELT SKEIAFYNRY NNIDSNIMAK SSSLKNLPNS TIYRLTEKFI
     NSLQNEYPTT VPTIFRTSDK LTPTDELCPE DSCMLCELPV DTIKKISANS SAINDLEYSL
     SISRLDNTAI EKLFVLKSVC YSCSVVLKDV GGHGNLPQTI KDKIKSRDNL KDKIKEFLIE
     D
//