ID A0A834NNM7_VESGE Unreviewed; 485 AA.
AC A0A834NNM7;
DT 29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 1.
DT 08-OCT-2025, entry version 9.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN ORFNames=HZH68_003111 {ECO:0000313|EMBL:KAF7414622.1};
OS Vespula germanica (German yellow jacket) (Paravespula germanica).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC Vespidae; Vespinae; Vespula.
OX NCBI_TaxID=30212 {ECO:0000313|EMBL:KAF7414622.1, ECO:0000313|Proteomes:UP000617340};
RN [1] {ECO:0000313|EMBL:KAF7414622.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Linc-1 {ECO:0000313|EMBL:KAF7414622.1};
RX PubMed=32859687; DOI=10.1534/g3.120.401579;
RA Harrop T.W.R., Guhlin J., McLaughlin G.M., Permina E., Stockwell P.,
RA Gilligan J., Le Lec M.F., Gruber M.A.M., Quinn O., Lovegrove M.,
RA Duncan E.J., Remnant E.J., Van Eeckhoven J., Graham B., Knapp R.A.,
RA Langford K.W., Kronenberg Z., Press M.O., Eacker S.M., Wilson-Rankin E.E.,
RA Purcell J., Lester P.J., Dearden P.K.;
RT "High-Quality Assemblies for Three Invasive Social Wasps from the
RT <i>Vespula</i> Genus.";
RL G3 (Bethesda) 10:3479-3488(2020).
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with NCS6/CTU1 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF7414622.1}.
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DR EMBL; JACSDZ010000002; KAF7414622.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A834NNM7; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000617340; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000617340};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 485 AA; 54580 MW; C62CA06B8834F86E CRC64;
MCSLNACSCD FNDDITPKID AIQILNSDVC KKCRTNSTEI ILRRKDGYCT SCFLINTTHK
FRAALGKSKI IHRGDTILIG HCGRDNSTAL LHLINSGMSE TTHKKIIFNV VIFFIDDGIV
EGYTVNERKK MLDTISKQVK MFGFVGYTAS LQESLRENDV PNIYLMDAPE VVIDESDVIS
NMLNNLADDS AREELLRQLR HRLLILAARK LECNKVFVAD CATNIAVNIL SDISLGRGAQ
LSPDIDFSDT RYTDVMLLRP MKDFTKEEII HYLSCNKLNA VRSSKQNRTS LSTSIQSLTN
KFISQLNTEF HGTVSTVFRT GGKLSTELLA CQNLEENSVC AMCNIPLDTK VTNNEISALQ
ATTFSALLSS NNIDIDNTKV KANDLLNVNP EELTVQVNHS EENNQNEHND SNITNGKQCT
WNIEEYKRFK TQSLSLNDIL KHLCYSCRLI FRSSDIKSIP SFVLNTIQTR LSHEIRPNEI
TDLLL
//
