ID A0A835BSJ9_9POAL Unreviewed; 635 AA.
AC A0A835BSJ9;
DT 29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 1.
DT 02-APR-2025, entry version 17.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN ORFNames=HU200_033051 {ECO:0000313|EMBL:KAF8702283.1};
OS Digitaria exilis.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Anthephorinae; Digitaria.
OX NCBI_TaxID=1010633 {ECO:0000313|EMBL:KAF8702283.1, ECO:0000313|Proteomes:UP000636709};
RN [1] {ECO:0000313|EMBL:KAF8702283.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaves {ECO:0000313|EMBL:KAF8702283.1};
RA Bennetzen J.L., Chen S., Ma X., Wang X., Yssel A.E.J., Chaluvadi S.R.,
RA Johnson M., Gangashetty P., Hamidou F., Sanogo M.D., Zwaenepoel A.,
RA Wallace J., Van De Peer Y., Van Deynze A.;
RT "Genome sequence and genetic diversity analysis of an under-domesticated
RT orphan crop, white fonio (Digitaria exilis).";
RL Submitted (JUL-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
CC -!- SIMILARITY: Belongs to the RBR family. RNF14 subfamily.
CC {ECO:0000256|ARBA:ARBA00044508}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF8702283.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JACEFO010001787; KAF8702283.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A835BSJ9; -.
DR OrthoDB; 1431934at2759; -.
DR Proteomes; UP000636709; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR CDD; cd20341; BRcat_RBR_RNF14; 1.
DR CDD; cd20354; Rcat_RBR_RNF14; 1.
DR CDD; cd23820; RWD_RNF14; 1.
DR FunFam; 1.20.120.1750:FF:000029; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.10.110.10:FF:000083; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000358; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR047548; Rcat_RBR_RNF14.
DR InterPro; IPR006575; RWD_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF22191; IBR_1; 1.
DR Pfam; PF05773; RWD; 1.
DR SMART; SM00647; IBR; 2.
DR SMART; SM00184; RING; 3.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF57850; RING/U-box; 4.
DR SUPFAM; SSF54495; UBC-like; 1.
DR PROSITE; PS50908; RWD; 1.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000636709};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 138..275
FT /note="RWD"
FT /evidence="ECO:0000259|PROSITE:PS50908"
FT DOMAIN 326..546
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 330..378
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 1..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 65..87
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..101
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 635 AA; 71010 MW; 98A4EE7DB3F1F7B7 CRC64;
MPRKSSRPRG AAAQGPVGPT AVPRPPFKGA APNPSPAAEP PQAAAPAVDE ALERLHLDTV
SDGDPPAPAP PEPEPEPEAP APSPPAPVEA SSSGRSAAGG SLEEEEALRK LHELAEAGGE
EVALTDDEVG ANDQRQEDEI CALESIFGDA VVIFNRKGGQ RCFQVHVHIE IPEAIDVSTR
FNYGDGTLKY GEASDADDLV YKFRVEHLPP ILLTCLLPAS YPSHRPPLFT ISSYWLDKGM
ISSLCHMLDM LWEEQQGMEV TYQWVQWLQS SSLSHLGFDN EIVLSKNDVA CEADKRACLD
NASPDVIIPR MMRYNENRHH EAFVNAIHDC MICFSECPGV DFVKLPCHHF FCWKCMQTYC
KMNVKEGNVV KLLCPDTKCE GAVPPNILKR LLGEDEFERW EGLLLQRTLD AMADVVYCPR
CQTACLEDVG NEAVCSSCLF SFCTLCRNRR HIGEQCMSPA ERLLILEKRQ QSGHVQADQM
RILEELRSLK EIMKDSKQCP KCNMAISKTE GCNKMECWNC KEYFCYQCNR AITGYDHFRG
SCVLFPQEEL DRWEMQMNQR VQRQVVAQVH AEMHLQHGPA HPCPTCRQPS PKIGNNNHLF
CWACQKHFCA LCHKSVPKPA QHYGPKGCKQ HTADT
//
