ID A0A851K3E7_VIDCH Unreviewed; 238 AA.
AC A0A851K3E7;
DT 29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 1.
DT 28-JAN-2026, entry version 12.
DE RecName: Full=Cyanocobalamin reductase / alkylcobalamin dealkylase {ECO:0000256|ARBA:ARBA00014027};
DE EC=1.16.1.6 {ECO:0000256|ARBA:ARBA00012666};
DE EC=2.5.1.151 {ECO:0000256|ARBA:ARBA00012308};
DE AltName: Full=Alkylcobalamin:glutathione S-alkyltransferase {ECO:0000256|ARBA:ARBA00032650};
DE AltName: Full=CblC {ECO:0000256|ARBA:ARBA00031815};
DE AltName: Full=Cyanocobalamin reductase (cyanide-eliminating) {ECO:0000256|ARBA:ARBA00031313};
DE AltName: Full=Methylmalonic aciduria and homocystinuria type C protein {ECO:0000256|ARBA:ARBA00031056};
DE Flags: Fragment;
GN Name=Mmachc {ECO:0000313|EMBL:NXB84437.1};
GN ORFNames=VIDCHA_R03181 {ECO:0000313|EMBL:NXB84437.1};
OS Vidua chalybeata (Village indigobird).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Neoaves; Telluraves; Australaves;
OC Passeriformes; Passeroidea; Estrildidae; Viduinae; Vidua.
OX NCBI_TaxID=81927 {ECO:0000313|EMBL:NXB84437.1, ECO:0000313|Proteomes:UP000634236};
RN [1] {ECO:0000313|EMBL:NXB84437.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=OUT-0048 {ECO:0000313|EMBL:NXB84437.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:NXB84437.1};
RA Zhang G.;
RT "Bird 10,000 Genomes (B10K) Project - Family phase.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 cob(II)alamin-[cyanocobalamin reductase] + 2 hydrogen
CC cyanide + NADP(+) = 2 cyanocob(III)alamin + 2 apo-[cyanocobalamin
CC reductase] + NADPH + H(+); Xref=Rhea:RHEA:16113, Rhea:RHEA-
CC COMP:14717, Rhea:RHEA-COMP:14718, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16304, ChEBI:CHEBI:17439, ChEBI:CHEBI:18407,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:83228; EC=1.16.1.6;
CC Evidence={ECO:0000256|ARBA:ARBA00047958};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:16115;
CC Evidence={ECO:0000256|ARBA:ARBA00047958};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=apo-[alkylcobalamin reductase] + adenosylcob(III)alamin +
CC glutathione = S-adenosylglutathione + cob(I)alamin-[alkylcobalamin
CC reductase] + H(+); Xref=Rhea:RHEA:63136, Rhea:RHEA-COMP:14730,
CC Rhea:RHEA-COMP:14731, ChEBI:CHEBI:15378, ChEBI:CHEBI:18408,
CC ChEBI:CHEBI:57925, ChEBI:CHEBI:60488, ChEBI:CHEBI:83228,
CC ChEBI:CHEBI:146184; EC=2.5.1.151;
CC Evidence={ECO:0000256|ARBA:ARBA00048537};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63137;
CC Evidence={ECO:0000256|ARBA:ARBA00048537};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=apo-[alkylcobalamin reductase] + an R-cob(III)alamin +
CC glutathione = cob(I)alamin-[alkylcobalamin reductase] + an S-
CC substituted glutathione + H(+); Xref=Rhea:RHEA:40719, Rhea:RHEA-
CC COMP:14730, Rhea:RHEA-COMP:14731, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57925, ChEBI:CHEBI:60488, ChEBI:CHEBI:83228,
CC ChEBI:CHEBI:90779, ChEBI:CHEBI:140785; EC=2.5.1.151;
CC Evidence={ECO:0000256|ARBA:ARBA00049505};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40720;
CC Evidence={ECO:0000256|ARBA:ARBA00049505};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=apo-[alkylcobalamin reductase] + methylcob(III)alamin +
CC glutathione = S-methyl glutathione + cob(I)alamin-[alkylcobalamin
CC reductase] + H(+); Xref=Rhea:RHEA:63132, Rhea:RHEA-COMP:14730,
CC Rhea:RHEA-COMP:14731, ChEBI:CHEBI:15378, ChEBI:CHEBI:28115,
CC ChEBI:CHEBI:57925, ChEBI:CHEBI:60488, ChEBI:CHEBI:83228,
CC ChEBI:CHEBI:141467; EC=2.5.1.151;
CC Evidence={ECO:0000256|ARBA:ARBA00047294};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63133;
CC Evidence={ECO:0000256|ARBA:ARBA00047294};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the MMACHC family.
CC {ECO:0000256|ARBA:ARBA00007762}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:NXB84437.1}.
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DR EMBL; WBNB01000369; NXB84437.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A851K3E7; -.
DR Proteomes; UP000634236; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0031419; F:cobalamin binding; IEA:UniProtKB-KW.
DR GO; GO:0033787; F:cyanocobalamin reductase (cyanide-eliminating) (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0032451; F:demethylase activity; IEA:TreeGrafter.
DR GO; GO:0071949; F:FAD binding; IEA:TreeGrafter.
DR GO; GO:0009235; P:cobalamin metabolic process; IEA:TreeGrafter.
DR CDD; cd12959; MMACHC-like; 1.
DR InterPro; IPR032037; MMACHC.
DR PANTHER; PTHR31457:SF2; CYANOCOBALAMIN REDUCTASE _ ALKYLCOBALAMIN DEALKYLASE; 1.
DR PANTHER; PTHR31457; METHYLMALONIC ACIDURIA AND HOMOCYSTINURIA TYPE C PROTEIN; 1.
DR Pfam; PF16690; MMACHC; 1.
PE 3: Inferred from homology;
KW Cobalamin {ECO:0000256|ARBA:ARBA00022628};
KW Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643}; NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000634236}.
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:NXB84437.1"
FT NON_TER 238
FT /evidence="ECO:0000313|EMBL:NXB84437.1"
SQ SEQUENCE 238 AA; 27889 MW; 3B640698F1CAABEF CRC64;
MERRVAEQLR SVLGPLGLEA HAFKVGWYNA VLQPAFHLPY PDDTLAFVVL STPSMFDKAL
KPFVKKERLK IIRDPVDQCV SYHLSRVKER FPDQRMDVMF DYEMLPSRKP KFLAQTAAHV
AGAAYYYQRK DVKLDPWGKK KIFGVCIHPK YGGWFAIRGL LLFPDIQVPF LEQRAPVDCV
STEEKRIELL EQFNFHWQDC RYRDIIEVKE RYSEEQKAYF ATPPAERFRL LGLSQEAQ
//
