ID A0A852N1K1_9PASS Unreviewed; 473 AA.
AC A0A852N1K1;
DT 29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 1.
DT 18-JUN-2025, entry version 17.
DE RecName: Full=E3 ubiquitin-protein ligase RNF14 {ECO:0000256|ARBA:ARBA00067098};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
DE AltName: Full=RING finger protein 14 {ECO:0000256|ARBA:ARBA00075528};
DE Flags: Fragment;
GN Name=Rnf14 {ECO:0000313|EMBL:NXY06163.1};
GN ORFNames=PTEMEL_R10972 {ECO:0000313|EMBL:NXY06163.1};
OS Pteruthius melanotis.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Neoaves; Telluraves; Australaves;
OC Passeriformes; Sylvioidea; Timaliidae; Pteruthius.
OX NCBI_TaxID=357074 {ECO:0000313|EMBL:NXY06163.1, ECO:0000313|Proteomes:UP000603297};
RN [1] {ECO:0000313|EMBL:NXY06163.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=B10K-IZ-033-77 {ECO:0000313|EMBL:NXY06163.1};
RA Zhang G.;
RT "Bird 10,000 Genomes (B10K) Project - Family phase.";
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBUNIT: Interacts with GCN1; interaction takes place in response to
CC ribosome collisions and is required for ubiquitination of EEF1A1/eEF1A.
CC Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2.
CC Interacts with AR/androgen receptor. Interacts with TCF7/TCF1,
CC TCF7L1/TCF3 and TCF7L2/TCF4; promoting Wnt signaling.
CC {ECO:0000256|ARBA:ARBA00062346}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RBR family. RNF14 subfamily.
CC {ECO:0000256|ARBA:ARBA00044508}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:NXY06163.1}.
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DR EMBL; WEIY01000307; NXY06163.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A852N1K1; -.
DR OrthoDB; 1431934at2759; -.
DR Proteomes; UP000603297; Unassembled WGS sequence.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR CDD; cd20341; BRcat_RBR_RNF14; 1.
DR CDD; cd20354; Rcat_RBR_RNF14; 1.
DR CDD; cd16628; RING-HC_RBR_RNF14; 1.
DR CDD; cd23820; RWD_RNF14; 1.
DR FunFam; 1.20.120.1750:FF:000011; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 2.20.25.20:FF:000007; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.10.110.10:FF:000049; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000186; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 2.20.25.20; -; 1.
DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR047548; Rcat_RBR_RNF14.
DR InterPro; IPR031128; RNF14_RING-HC_Zfn.
DR InterPro; IPR006575; RWD_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF22191; IBR_1; 1.
DR Pfam; PF05773; RWD; 1.
DR SMART; SM00647; IBR; 2.
DR SMART; SM00591; RWD; 1.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR SUPFAM; SSF54495; UBC-like; 1.
DR PROSITE; PS50908; RWD; 1.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Ligase {ECO:0000313|EMBL:NXY06163.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000603297};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 11..137
FT /note="RWD"
FT /evidence="ECO:0000259|PROSITE:PS50908"
FT DOMAIN 216..457
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 220..265
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:NXY06163.1"
FT NON_TER 473
FT /evidence="ECO:0000313|EMBL:NXY06163.1"
SQ SEQUENCE 473 AA; 53456 MW; 34838A377FA15653 CRC64;
MSSEDKEAQE DELLALASIY DEDEFKRAQS AQGGETRICL ELPQDFKIFV RGDSTESLQS
SGFEYSVCFL PPLVLNFELP PDYPSTSPPV FTLSGKWLSQ AQLSALCKHL DNVWEENRGC
VVLFAWMQFL KEETLNYLNI SSPYELKLCP QGKGHSRTPV GPLEAGKDGG GATGSATAEE
EIVDARAVQD VESLSSLIRE ILDFDQAQRR KCFNSKMYSC SICFCEKLGS ECMHFTECSH
VYCKACLKDY FEIQIRDGQV HCLNCPEPKC SSVATPGQVK ELVGEELFAR YDRLLLQSSL
DLMADVVYCP RPGCQTPVMQ EPGCTMGICS CCNYAFCTLC KMTYHGVSPC KVTAEKLMDL
RNEYLEADET NKRFLEQRYG KRVIQKALEE MESKEWLEKN SKSCPCCGTH IEKLDGCNKM
TCTGCMQYFC WLCMGSLSRA NPYRHFNDPS SPCFNRLFQA MHIDDGEFWE VED
//
