ID A0A8B2P0R6_9HYPH Unreviewed; 304 AA.
AC A0A8B2P0R6;
DT 29-SEP-2021, integrated into UniProtKB/TrEMBL.
DT 29-SEP-2021, sequence version 1.
DT 08-OCT-2025, entry version 15.
DE RecName: Full=Arginase {ECO:0000256|ARBA:ARBA00018123, ECO:0000256|NCBIfam:TIGR01229};
DE EC=3.5.3.1 {ECO:0000256|ARBA:ARBA00012168, ECO:0000256|NCBIfam:TIGR01229};
GN Name=rocF {ECO:0000313|EMBL:RAI04580.1};
GN ORFNames=DLJ53_08175 {ECO:0000313|EMBL:RAI04580.1};
OS Acuticoccus sediminis.
OC Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC Hyphomicrobiales; Amorphaceae; Acuticoccus.
OX NCBI_TaxID=2184697 {ECO:0000313|EMBL:RAI04580.1, ECO:0000313|Proteomes:UP000249590};
RN [1] {ECO:0000313|EMBL:RAI04580.1, ECO:0000313|Proteomes:UP000249590}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PTG4-2 {ECO:0000313|EMBL:RAI04580.1,
RC ECO:0000313|Proteomes:UP000249590};
RA Liu X., Lai Q., Du Y., Sun F., Zhang X., Wang S., Shao Z.;
RT "Acuticoccus sediminis sp. nov., isolated from deep-sea sediment of Indian
RT Ocean.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-arginine + H2O = urea + L-ornithine; Xref=Rhea:RHEA:20569,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:16199, ChEBI:CHEBI:32682,
CC ChEBI:CHEBI:46911; EC=3.5.3.1;
CC Evidence={ECO:0000256|ARBA:ARBA00047391,
CC ECO:0000256|RuleBase:RU361159};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|PIRSR:PIRSR036979-1,
CC ECO:0000256|RuleBase:RU361159};
CC Note=Binds 2 manganese ions per subunit.
CC {ECO:0000256|PIRSR:PIRSR036979-1, ECO:0000256|RuleBase:RU361159};
CC -!- PATHWAY: Nitrogen metabolism; urea cycle; L-ornithine and urea from L-
CC arginine: step 1/1. {ECO:0000256|ARBA:ARBA00005098}.
CC -!- SIMILARITY: Belongs to the arginase family. {ECO:0000256|PROSITE-
CC ProRule:PRU00742, ECO:0000256|RuleBase:RU003684}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RAI04580.1}.
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DR EMBL; QHHQ01000001; RAI04580.1; -; Genomic_DNA.
DR RefSeq; WP_111343863.1; NZ_QHHQ01000001.1.
DR AlphaFoldDB; A0A8B2P0R6; -.
DR OrthoDB; 9788689at2; -.
DR UniPathway; UPA00158; UER00270.
DR Proteomes; UP000249590; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter.
DR GO; GO:0004053; F:arginase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0030145; F:manganese ion binding; IEA:TreeGrafter.
DR GO; GO:0006525; P:arginine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0000050; P:urea cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd09989; Arginase; 1.
DR FunFam; 3.40.800.10:FF:000012; Arginase; 1.
DR Gene3D; 3.40.800.10; Ureohydrolase domain; 1.
DR InterPro; IPR014033; Arginase.
DR InterPro; IPR006035; Ureohydrolase.
DR InterPro; IPR023696; Ureohydrolase_dom_sf.
DR InterPro; IPR020855; Ureohydrolase_Mn_BS.
DR NCBIfam; TIGR01229; rocF_arginase; 1.
DR PANTHER; PTHR43782; ARGINASE; 1.
DR PANTHER; PTHR43782:SF3; ARGINASE; 1.
DR Pfam; PF00491; Arginase; 1.
DR PIRSF; PIRSF036979; Arginase; 1.
DR PRINTS; PR00116; ARGINASE.
DR SUPFAM; SSF52768; Arginase/deacetylase; 1.
DR PROSITE; PS01053; ARGINASE_1; 1.
DR PROSITE; PS51409; ARGINASE_2; 1.
PE 3: Inferred from homology;
KW Arginine metabolism {ECO:0000256|ARBA:ARBA00022503,
KW ECO:0000256|RuleBase:RU361159};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU003684};
KW Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|PIRSR:PIRSR036979-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR036979-1};
KW Reference proteome {ECO:0000313|Proteomes:UP000249590}.
FT BINDING 98
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR036979-1"
FT BINDING 121
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR036979-1"
FT BINDING 123
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR036979-1"
FT BINDING 125
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR036979-1"
FT BINDING 224
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR036979-1"
FT BINDING 226
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR036979-1"
SQ SEQUENCE 304 AA; 32155 MW; 1D64A4D2F61CE232 CRC64;
MATMSVLGAP TETGTYRRGC IMGPAAYRTA GLIESLRDLG HVIAEDRDLV PDTVEIDDAA
HAPAHGVGAT AGWVRAVRRA FAQALARGNV PVLLGGDHSI AAGSLAALMD VAPGTHIVWL
DAHPDFNTIE TTMSGNLHGI PVAIASGLPG ADLIYGGPIT PLDPRQITMI GIRSVDPDER
KLLKSHGVTV HDMRDVDEFG MGELIRRAIR RAVEAGARLH VSLDVDFLDP DVAPAVGTTV
PGGATVREAH LAMELLHESG LVASLDLVEL NPFLDERGRT ATLMVDLAAS LFGRTVMDRP
TTTW
//
