ID A0A8B8FJL4_9HEMI Unreviewed; 1238 AA.
AC A0A8B8FJL4;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 28-JAN-2026, entry version 20.
DE RecName: Full=Isoleucine--tRNA ligase, cytoplasmic {ECO:0000256|ARBA:ARBA00069879};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN Name=LOC112684021 {ECO:0000313|RefSeq:XP_025411099.1};
OS Sipha flava (yellow sugarcane aphid).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Paraneoptera; Hemiptera; Sternorrhyncha; Aphidomorpha;
OC Aphidoidea; Aphididae; Sipha.
OX NCBI_TaxID=143950 {ECO:0000313|Proteomes:UP000694846, ECO:0000313|RefSeq:XP_025411099.1};
RN [1] {ECO:0000313|RefSeq:XP_025411099.1}
RP IDENTIFICATION.
RC TISSUE=Whole body {ECO:0000313|RefSeq:XP_025411099.1};
RG RefSeq;
RL Submitted (AUG-2025) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP +
CC diphosphate; Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666, Rhea:RHEA-
CC COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58045,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78528, ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00048359};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR RefSeq; XP_025411099.1; XM_025555314.1.
DR AlphaFoldDB; A0A8B8FJL4; -.
DR GeneID; 112684021; -.
DR CTD; 45785; -.
DR OrthoDB; 1706657at2759; -.
DR Proteomes; UP000694846; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA deacylase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR FunFam; 1.10.730.10:FF:000004; Isoleucyl-tRNA synthetase, cytoplasmic; 1.
DR FunFam; 3.40.50.620:FF:000023; Isoleucyl-tRNA synthetase,cytoplasmic; 1.
DR FunFam; 3.40.50.620:FF:000050; Isoleucyl-tRNA synthetase,cytoplasmic; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.730.10; Isoleucyl-tRNA Synthetase, Domain 1; 1.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR057033; Ubiquitin_IARS1.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF23567; Ubiquitin_IARS1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000694846}.
FT DOMAIN 17..637
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 690..838
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT DOMAIN 1082..1163
FT /note="Isoleucine--tRNA ligase cytoplasmic ubiquitin-like"
FT /evidence="ECO:0000259|Pfam:PF23567"
SQ SEQUENCE 1238 AA; 142781 MW; 1DC55C8AA6E12C49 CRC64;
MVEPAVEYLN FPKEEEKILE FWKEIDVFHE CLRQSKGKPR FSFYDGPPFA TGLPHYGHIL
AGAIKDVITR YAHQTGHHVE RRFGWDTHGL PVEFEIDKLL NIKGPEDVAK MGIDKYNAEC
RKIVMRYAND WETIITRLGR WIDFKNDYKT LYPWFMESVW WIFSELWNKG LVYRGVKVMP
FSTACSTPLS NFESGQNYKE VVDPAVTVSF PLESDPSVSL IAWTTTPWTL PSNLALCVNP
TLIYVKIRDL NDKILILMEA RLDVLFKKKE DYTILDKFPG EKLKGLKYKP LFPYFIQSQK
TAFVVLTDGY VSAESGTGIV HQAPYFGEDD YRVCLAAGVI TRDQDIVCPV DESGKFVLPV
SDFQGQYVKD ADKNIIKHIK EAGRLVNASS VKHSYPFCWR SETPLIYKAV PSWFIRVQHM
SQDLLTSNKS TYWVPNFVKE KRFGNWLKDA RDWAISRNRY WGTPIPLWVS EDGKEIVCIS
SIDQLYKMTG VLVTDLHRET VDKLTIPSVR PGCPPLRRIT EVFDCWFESG SMPYAQQHYP
FENRKEFLEK FPADFIAEGI DQTRGWFYTL LVISTALFNK PPFKNLIANG LVLASDGQKM
SKRKKNYPDP LEVVNKFGAD ALRLYLINSP VVRAENLRFK EEGVKEILKD LFLPWYNAYR
FFLQNIRDSY GNMYQPNKNY YSAPSENKMD RWITSWTQSL IAFVRQEMAA YRLYTVAPRL
VKFIDVLTNW YVRLNRQRLK GECGQTDWQQ SLDTLYHVLM NMVCLMAPYT PFICELMYQN
LKKIENLEER SVHFHMVPIV QDHLIDKNIE QSVSNMQNII EMGRVMRDRK TIPVKYPLPD
LLIVADDPKI IENVEEFRNY IEKELNVKTI NFTKNKSRYV KLRAEPDHKT LGSRLKSDFK
RVTAAIRELT NEQLQSWQNE VSTMTNGSDD AKKPSLKVLE YELDASEFRI LYDFTGSEAE
EMAKKYEAQL NNDILVLLNV SPDQNMLDEG TAREIINRVQ KLRKKAHLVP TDLITVYYNV
NPAGELSRVA KEWNDFIINV LKVPFIDGQA VGNIIIEETQ TLKGSNLKIV LTKENNDQTP
MPVCKFVNVE LHGLKARYGA SNSSTILLEN PVGKNVLNVI GLTKEIKNAF GLFGIDILLK
NVDGKMLDDN LLNTVNNGTI IVQKNNNSPE CCKIQQKTSP ISQFINAKYG SEIGTILLHG
EDDKNNNDKL KLKIAHTFNL KANQIILKQE KDFVIVHS
//
