ID A0A8C6KL04_NOTFU Unreviewed; 427 AA.
AC A0A8C6KL04;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 28-JAN-2026, entry version 19.
DE RecName: Full=DNA primase large subunit {ECO:0000256|ARBA:ARBA00019038};
DE AltName: Full=DNA primase 58 kDa subunit {ECO:0000256|ARBA:ARBA00080636};
GN Name=PRIM2 {ECO:0000313|Ensembl:ENSNFUP00015007194.1};
GN Synonyms=prim2 {ECO:0000313|Ensembl:ENSNFUP00015007194.1};
OS Nothobranchius furzeri (Turquoise killifish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Cyprinodontiformes; Nothobranchiidae;
OC Nothobranchius.
OX NCBI_TaxID=105023 {ECO:0000313|Ensembl:ENSNFUP00015007194.1, ECO:0000313|Proteomes:UP000694548};
RN [1] {ECO:0000313|Ensembl:ENSNFUP00015007194.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GRZ {ECO:0000313|Ensembl:ENSNFUP00015007194.1};
RA Senf B., Petzold A., Downie B.R., Koch P., Platzer M.;
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSNFUP00015007194.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (AUG-2025) to UniProtKB.
RN [3] {ECO:0000313|Ensembl:ENSNFUP00015007194.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2025) to UniProtKB.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBUNIT: Heterodimer of a catalytic subunit PRIM1 and a regulatory
CC subunit PRIM2, also known as the DNA primase complex. Interacts via (C-
CC terminus) with PRIM1. Component of the alpha DNA polymerase complex
CC (also known as the alpha DNA polymerase-primase complex) consisting of
CC four subunits: the catalytic subunit POLA1, the regulatory subunit
CC POLA2, and the primase complex subunits PRIM1 and PRIM2 respectively.
CC Within the complex, POLA1 directly interacts with PRIM2.
CC {ECO:0000256|ARBA:ARBA00061902}.
CC -!- SIMILARITY: Belongs to the eukaryotic-type primase large subunit
CC family. {ECO:0000256|ARBA:ARBA00010564}.
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DR AlphaFoldDB; A0A8C6KL04; -.
DR Ensembl; ENSNFUT00015007570.1; ENSNFUP00015007194.1; ENSNFUG00015003554.1.
DR GeneTree; ENSGT00390000009790; -.
DR Proteomes; UP000694548; Chromosome sgr03.
DR GO; GO:0005658; C:alpha DNA polymerase:primase complex; IEA:UniProtKB-ARBA.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006270; P:DNA replication initiation; IEA:TreeGrafter.
DR GO; GO:0006269; P:DNA replication, synthesis of primer; IEA:UniProtKB-KW.
DR CDD; cd07322; PriL_PriS_Eukaryotic; 1.
DR FunFam; 1.20.930.80:FF:000001; DNA primase large subunit; 1.
DR Gene3D; 1.20.930.80; -; 1.
DR InterPro; IPR058560; DNA_primase_C.
DR InterPro; IPR016558; DNA_primase_lsu_euk.
DR InterPro; IPR007238; DNA_primase_lsu_euk/arc.
DR PANTHER; PTHR10537; DNA PRIMASE LARGE SUBUNIT; 1.
DR PANTHER; PTHR10537:SF3; DNA PRIMASE LARGE SUBUNIT; 1.
DR Pfam; PF04104; DNA_primase_lrg; 1.
DR Pfam; PF26466; DNA_primase_lrg_N; 1.
DR PIRSF; PIRSF009449; DNA_primase_large_subunit; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|PIRSR:PIRSR009449-1};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR009449-1};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRSR:PIRSR009449-
KW 1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR009449-1};
KW Primosome {ECO:0000256|ARBA:ARBA00022515};
KW Reference proteome {ECO:0000313|Proteomes:UP000694548}.
FT DOMAIN 274..409
FT /note="DNA primase large subunit C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04104"
FT BINDING 283
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 363
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 380
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
SQ SEQUENCE 427 AA; 49781 MW; 2C3ED8C1F365556C CRC64;
ISTMLFSGRR KKFGTSIQTE LYVQPLQFYG QPPLENISLS EFETFAVDRL KLLKTVENLG
VSYVKQSEQY SKKLDSELKT LNFPYRSESE KRRKDHISHF ILRLAYCQTE DLRRWFIQQE
VDLFRYRFNG LLSKQKLEFL HKNNLQYDAI SLKEKKELKE KLVSSTYAIS GITVEDQDFY
KVPFQDALDL VRTRKVYLKE GYAYIPHQDI VTIVLNDFRT RLSKALALTA RSLPAVHSDE
RLQPLLNHLS HAYLGQDYSI QKNVGKISLE QIDSLSGKSF PLCMRQLHQA LRENHHLRHG
GRMQYGLFLK GIGLSLEQAL QFWRSEFIRG KVDGDKFDKA YAYSIRHMFG KEGKRTDYTP
YSCMKVILSN PPSQGDYHGC PFRHSDPELL KQKLQVFKVS PSGIVQVGDT QTIIICFQQH
NLDCYFF
//
