ID A0A8C7FZI5_ONCKI Unreviewed; 863 AA.
AC A0A8C7FZI5;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 28-JAN-2026, entry version 19.
DE RecName: Full=Protein argonaute-4 {ECO:0000256|HAMAP-Rule:MF_03033};
DE Short=Argonaute4 {ECO:0000256|HAMAP-Rule:MF_03033};
DE AltName: Full=Eukaryotic translation initiation factor 2C 4 {ECO:0000256|HAMAP-Rule:MF_03033};
DE Short=eIF-2C 4 {ECO:0000256|HAMAP-Rule:MF_03033};
DE Short=eIF2C 4 {ECO:0000256|HAMAP-Rule:MF_03033};
GN Name=EIF2C4 {ECO:0000256|HAMAP-Rule:MF_03033};
GN Synonyms=AGO4 {ECO:0000256|HAMAP-Rule:MF_03033,
GN ECO:0000313|Ensembl:ENSOKIP00005035545.1}, LOC109903691
GN {ECO:0000313|Ensembl:ENSOKIP00005035545.1};
OS Oncorhynchus kisutch (Coho salmon) (Salmo kisutch).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC Salmonidae; Salmoninae; Oncorhynchus.
OX NCBI_TaxID=8019 {ECO:0000313|Ensembl:ENSOKIP00005035545.1, ECO:0000313|Proteomes:UP000694557};
RN [1] {ECO:0000313|Ensembl:ENSOKIP00005035545.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (AUG-2025) to UniProtKB.
RN [2] {ECO:0000313|Ensembl:ENSOKIP00005035545.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2025) to UniProtKB.
CC -!- FUNCTION: Required for RNA-mediated gene silencing (RNAi). Binds to
CC short RNAs such as microRNAs (miRNAs) and represses the translation of
CC mRNAs which are complementary to them. Lacks endonuclease activity and
CC does not appear to cleave target mRNAs. {ECO:0000256|HAMAP-
CC Rule:MF_03033}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, P-body {ECO:0000256|ARBA:ARBA00004201,
CC ECO:0000256|HAMAP-Rule:MF_03033}.
CC -!- SIMILARITY: Belongs to the argonaute family. Ago subfamily.
CC {ECO:0000256|ARBA:ARBA00008201, ECO:0000256|HAMAP-Rule:MF_03033}.
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DR AlphaFoldDB; A0A8C7FZI5; -.
DR Ensembl; ENSOKIT00005037523.1; ENSOKIP00005035545.1; ENSOKIG00005014889.1.
DR GeneTree; ENSGT00940000158729; -.
DR Proteomes; UP000694557; Unassembled WGS sequence.
DR GO; GO:0000932; C:P-body; IEA:UniProtKB-SubCell.
DR GO; GO:0016442; C:RISC complex; IEA:UniProtKB-UniRule.
DR GO; GO:0035198; F:miRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0035278; P:miRNA-mediated gene silencing by inhibition of translation; IEA:UniProtKB-UniRule.
DR CDD; cd02846; PAZ_argonaute_like; 1.
DR CDD; cd04657; Piwi_ago-like; 1.
DR FunFam; 2.170.260.10:FF:000001; Protein argonaute-2; 1.
DR FunFam; 3.30.420.10:FF:000001; Protein argonaute-2; 1.
DR FunFam; 3.40.50.2300:FF:000005; Protein argonaute-2; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR Gene3D; 2.170.260.10; paz domain; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR HAMAP; MF_03033; AGO4; 1.
DR InterPro; IPR028604; AGO4.
DR InterPro; IPR014811; ArgoL1.
DR InterPro; IPR032472; ArgoL2.
DR InterPro; IPR032473; Argonaute_Mid_dom.
DR InterPro; IPR032474; Argonaute_N.
DR InterPro; IPR003100; PAZ_dom.
DR InterPro; IPR036085; PAZ_dom_sf.
DR InterPro; IPR003165; Piwi.
DR InterPro; IPR045246; Piwi_ago-like.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR22891; EUKARYOTIC TRANSLATION INITIATION FACTOR 2C; 1.
DR Pfam; PF08699; ArgoL1; 1.
DR Pfam; PF16488; ArgoL2; 1.
DR Pfam; PF16487; ArgoMid; 1.
DR Pfam; PF16486; ArgoN; 1.
DR Pfam; PF02170; PAZ; 1.
DR Pfam; PF02171; Piwi; 1.
DR SMART; SM01163; DUF1785; 1.
DR SMART; SM00949; PAZ; 1.
DR SMART; SM00950; Piwi; 1.
DR SUPFAM; SSF101690; PAZ domain; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS50821; PAZ; 1.
DR PROSITE; PS50822; PIWI; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03033};
KW Reference proteome {ECO:0000313|Proteomes:UP000694557};
KW Ribonucleoprotein {ECO:0000256|ARBA:ARBA00023274, ECO:0000256|HAMAP-
KW Rule:MF_03033};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_03033};
KW RNA-mediated gene silencing {ECO:0000256|ARBA:ARBA00023158,
KW ECO:0000256|HAMAP-Rule:MF_03033};
KW Translation regulation {ECO:0000256|ARBA:ARBA00022845, ECO:0000256|HAMAP-
KW Rule:MF_03033}.
FT DOMAIN 221..340
FT /note="PAZ"
FT /evidence="ECO:0000259|PROSITE:PS50821"
FT DOMAIN 511..822
FT /note="Piwi"
FT /evidence="ECO:0000259|PROSITE:PS50822"
SQ SEQUENCE 863 AA; 97375 MW; 0C895702AD9AA2EB CRC64;
TVIPTVSAYP HLALLPSPPR RPGMGTVGKP IRLLANHFQV QIPKIDVYHY DIDIKPEKRP
RRVNREVVDT MVRHFKMQIF GDRQPGYDGK RNMYTAHPLP IGRDRVDLEV TLPGEGKDQT
FKVSLQWVSV VSLQMLLEAL SGHNEVPEDS VQALDVITRH LPSMRYTPVG RSFFSPPEGY
YHPLGGGREV WFGFHQSVRP AMWNMMLNID VSATAFYRAQ PVIEFMCEVL DIQNINEQTK
PLTDSQRVKF TKEIRGLKVE VTHCGQMKRK YRVCNVTRRP ASHQTFPLQL ENGQAMECTV
AQYFKQKYSL QLKYPHLPCL QVGQEQKHTY LPLEVCNIVA GQRCIKKLTD NQTSTMIKAT
ARSAPDRQEE ISRLVKSNSM VGGPDPYLKE FGIVVHNDMT EVTGRVLPAP MLQYGGRNKT
VATPNQGVWD MRGKQFYAGI EIKVWAVACF APQKQCREDL LKSFTDQLRK ISKDAGMPIQ
GQPCFCKYAQ GADSVEPMFK HLKMSYVGLQ LIVVILPGKT PVYAEVKRVG DTLLGMATQC
VQVKNVVKTS PQTLSNLCLK INAKLGGINN VLVPHQRPSV FQQPVIFLGA DVTHPPAGDG
KKPSIAAVVG SMDGHPSRYC ATVRVQTSRQ DLSQEQLYSQ EVIQDLTNMV RELLIQFYKS
TRFKPTRIIY YRGGVSEGQM KQVAWPELIA IRKACISLEE DYRPGITYIV VQKRHHTRLF
CSDKAERVGK SGNVPAGTTV DSTITHPSEF DFYLCSHAGI QGTSRPSHYH VLWDDNCFTA
DELQLLTYQL CHTYVRCTRS VSIPAPAYYA RLVAFRARYH LVDKDHDSAE GSHVSGQSNG
RDPLALAKAV QIHYDTQHTM YFA
//
