ID A0A8C7MVL0_ONCKI Unreviewed; 319 AA.
AC A0A8C7MVL0;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 28-JAN-2026, entry version 18.
DE RecName: Full=Phosphopantothenate--cysteine ligase {ECO:0000256|ARBA:ARBA00068949};
DE EC=6.3.2.51 {ECO:0000256|ARBA:ARBA00066585};
DE AltName: Full=Phosphopantothenoylcysteine synthetase {ECO:0000256|ARBA:ARBA00080986};
GN Name=PPCS {ECO:0000313|Ensembl:ENSOKIP00005082636.1};
GN Synonyms=ppcs {ECO:0000313|Ensembl:ENSOKIP00005082636.1};
OS Oncorhynchus kisutch (Coho salmon) (Salmo kisutch).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC Salmonidae; Salmoninae; Oncorhynchus.
OX NCBI_TaxID=8019 {ECO:0000313|Ensembl:ENSOKIP00005082636.1, ECO:0000313|Proteomes:UP000694557};
RN [1] {ECO:0000313|Ensembl:ENSOKIP00005082636.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (AUG-2025) to UniProtKB.
RN [2] {ECO:0000313|Ensembl:ENSOKIP00005082636.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2025) to UniProtKB.
CC -!- FUNCTION: Catalyzes the second step in the biosynthesis of coenzyme A
CC from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate
CC to form 4-phosphopantothenoylcysteine. Has a preference for ATP over
CC CTP as a cosubstrate. {ECO:0000256|ARBA:ARBA00060296}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-4'-phosphopantothenate + L-cysteine + ATP = N-[(R)-4-
CC phosphopantothenoyl]-L-cysteine + AMP + diphosphate + H(+);
CC Xref=Rhea:RHEA:25156, ChEBI:CHEBI:10986, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:35235,
CC ChEBI:CHEBI:59458, ChEBI:CHEBI:456215; EC=6.3.2.51;
CC Evidence={ECO:0000256|ARBA:ARBA00051127};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25157;
CC Evidence={ECO:0000256|ARBA:ARBA00051127};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4-
CC phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H(+);
CC Xref=Rhea:RHEA:19397, ChEBI:CHEBI:10986, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:35235, ChEBI:CHEBI:37563,
CC ChEBI:CHEBI:59458, ChEBI:CHEBI:60377;
CC Evidence={ECO:0000256|ARBA:ARBA00052332};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19398;
CC Evidence={ECO:0000256|ARBA:ARBA00052332};
CC -!- SIMILARITY: Belongs to the PPC synthetase family.
CC {ECO:0000256|ARBA:ARBA00005703}.
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DR RefSeq; XP_020338612.1; XM_020483023.2.
DR AlphaFoldDB; A0A8C7MVL0; -.
DR Ensembl; ENSOKIT00005088199.1; ENSOKIP00005082636.1; ENSOKIG00005035858.1.
DR GeneID; 109890900; -.
DR CTD; 79717; -.
DR GeneTree; ENSGT00950000182834; -.
DR Proteomes; UP000694557; Unassembled WGS sequence.
DR GO; GO:0004632; F:phosphopantothenate--cysteine ligase activity; IEA:UniProtKB-ARBA.
DR GO; GO:0015937; P:coenzyme A biosynthetic process; IEA:UniProtKB-ARBA.
DR FunFam; 3.40.50.10300:FF:000002; Phosphopantothenate--cysteine ligase 2; 1.
DR Gene3D; 3.40.50.10300; CoaB-like; 1.
DR InterPro; IPR035929; CoaB-like_sf.
DR InterPro; IPR007085; DNA/pantothenate-metab_flavo_C.
DR PANTHER; PTHR12290; CORNICHON-RELATED; 1.
DR Pfam; PF04127; DFP; 2.
DR SUPFAM; SSF102645; CoaB-like; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000694557}.
FT DOMAIN 43..91
FT /note="DNA/pantothenate metabolism flavoprotein C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04127"
FT DOMAIN 177..272
FT /note="DNA/pantothenate metabolism flavoprotein C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04127"
SQ SEQUENCE 319 AA; 35028 MW; 188FFAEE2E7B9C91 CRC64;
MADPRTLTAE GKLSEEFAVP SHVEEVKGQM AAFAVQHRAA GHRVVLITSG GTKVPLESRT
VRFLDNFSSG RRGASSAEYF LESGYAVIFL HRHRSLYPFT RLYSGINLLD SLQLESGKGG
SVSDQVVVNQ QALPNIAKIL MRYQAVKKAG LLLPVEFSTL SEYLHLLKAA AQALSPIGSN
AMFYLAAAVS DFYIPASDMP EHKIQSSNGP LQISMKMVPK MLSPLVKDWA PQAFVISFKL
ETDPSILLDR ARRALATYRH QAVVANVLDT RRGYVVVVTP DTQAELVLTD EEASRDVEIE
DRIVSNLTAA HSQFITQQG
//
