ID A0A8E0QVG9_9EURO Unreviewed; 885 AA.
AC A0A8E0QVG9;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 18-JUN-2025, entry version 15.
DE SubName: Full=Multisite-specific tRNA:(Cytosine-C(5))-methyltransferase {ECO:0000313|EMBL:GFF23538.1};
GN ORFNames=Aud_008147 {ECO:0000313|EMBL:GIC91698.1}, IFM46972_00713
GN {ECO:0000313|EMBL:GFF23538.1};
OS Aspergillus udagawae.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=91492 {ECO:0000313|EMBL:GIC91698.1, ECO:0000313|Proteomes:UP000036893};
RN [1] {ECO:0000313|Proteomes:UP000036893}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 46973 {ECO:0000313|Proteomes:UP000036893};
RA Kusuya Y., Takahashi-Nakaguchi A., Takahashi H., Yaguchi T.;
RT "Draft Genome Sequence of the Pathogenic Filamentous Fungus Aspergillus
RT udagawae Strain IFM 46973T.";
RL Genome Announc. 3:e00834-15(2015).
RN [2] {ECO:0000313|EMBL:GIC91698.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IFM 46973 {ECO:0000313|EMBL:GIC91698.1};
RX PubMed=26251487; DOI=10.1128/genomeA.00834-15;
RA Kusuya Y., Takahashi-Nakaguchi A., Takahashi H., Yaguchi T.;
RT "Draft Genome Sequence of the Pathogenic Filamentous Fungus Aspergillus
RT udagawae Strain IFM 46973T.";
RL Genome Announc. 3:e00834-e00815(2015).
RN [3] {ECO:0000313|EMBL:GFF23538.1, ECO:0000313|Proteomes:UP000465221}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 46972 {ECO:0000313|EMBL:GFF23538.1,
RC ECO:0000313|Proteomes:UP000465221};
RA Takahashi H., Yaguchi T.;
RT "Draft genome sequence of Aspergillus udagawae IFM 46972.";
RL Submitted (JAN-2020) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:GIC91698.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IFM 46973 {ECO:0000313|EMBL:GIC91698.1};
RA Takahashi H., Umemura M., Ninomiya A., Kusuya Y., Urayama S., Shimizu M.,
RA Watanabe A., Kamei K., Yaguchi T., Hagiwara D.;
RT "Pan-genome distribution and transcriptional activeness of fungal secondary
RT metabolism genes in Aspergillus section Fumigati.";
RL Submitted (JAN-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|ARBA:ARBA00007494,
CC ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GIC91698.1}.
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DR EMBL; BLKC01000003; GFF23538.1; -; Genomic_DNA.
DR EMBL; BBXM02000006; GIC91698.1; -; Genomic_DNA.
DR RefSeq; XP_043148964.1; XM_043293029.1.
DR GeneID; 66995624; -.
DR Proteomes; UP000036893; Unassembled WGS sequence.
DR Proteomes; UP000465221; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016428; F:tRNA (cytidine-5-)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030488; P:tRNA methylation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR023270; RCMT_NCL1.
DR InterPro; IPR018314; RsmB/NOL1/NOP2-like_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22808; NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR22808:SF1; RNA CYTOSINE-C(5)-METHYLTRANSFERASE NSUN2-RELATED; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF25376; Pre-PUA_NSUN2; 1.
DR Pfam; PF25378; PUA_NSUN2; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR PRINTS; PR02011; RCMTNCL1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; tRNA processing {ECO:0000256|ARBA:ARBA00022694};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555}.
FT DOMAIN 50..455
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 530..623
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 816..885
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..10
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 548..557
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 581..593
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 816..826
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 865..875
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 339
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 168..174
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 226
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 253
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 286
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 885 AA; 99572 MW; A3A023E0F39D58B2 CRC64;
MAKRGGRKGG RGGGARGQRQ NYADIPKTNE KLERFYTEQG FIPEEEREAF WEALRRDLPN
SFRFTGSKGH ALAVQEKLKN HYIPEITSIQ YEGNFVEPPR PVPWYPDQLA WWMTTPKNVV
RRFKPFASFQ KFLVAETDVG NITRQEVVSM IPPLLIDVRP GMTVLDMCAA PGSKSAQLME
LLHAGEEDAM RQVAQQVKEG TAGPEPIGPE GLNDDGRTTG LLIANDSDFK RAHMLVHQMK
RLSSPNLIVT NHDATMYPSI RLPPLPESDG KPQKNRYLKF DRILADVPCT GDGTARKNIS
VWKEWGPANA LGLHATQVRI LVRALQMLKV GGRVVYSTCS MNPVENEAVV ASAINRCGGS
ANVEILDCSD ELPGLKRAAG VRTWKVMDRE GRMYNSWKEV EEQREREGIN GLGRIGEGMF
PPTGENADLP LERCIRVYPH LQDTGGFFIT VLEKKSEIRA KPEDSSKVIP KASIAALTEE
LDFKQKHGNA RPLEKLNALD DLVAPDQEAQ LEMDKNASVA EATHQVPYFA TNQISPAKRD
AEDMEDEVPS KKTKVEDGTE VVLGDRPIHR PPPEAEDPDV SESTPTPLPT ETPQAESTET
KPQPPQKRKP GQPIEEPFKY LDPDNEEIDP IFKFYEISER FPRDRFMVRN AQATPTRTIY
YTSALARDIL TANEGHGMKF VHCGVKMFVK QDAQRPNVCR WRIQTDGLRI LEPWVGPGRA
VVLTKKETLR KLLVEMFPKV ADDSWKDLGE IGERVRDIEM GCSILYVQPE ENFSEHMVLP
LWRSLHSVNL MLPKEERRAM LLRIFNDEPQ LLNITQQGTP QTSTPVTPAV ETNEEDVSGM
APTEAEENAL KQENLVLGED QQEQVDARET WTKAGDEEDR FNTTV
//
