ID A0A8H4R0I0_9AGAR Unreviewed; 1078 AA.
AC A0A8H4R0I0;
DT 19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2022, sequence version 1.
DT 28-JAN-2026, entry version 11.
DE RecName: Full=Isoleucine--tRNA ligase, cytoplasmic {ECO:0000256|ARBA:ARBA00069879};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=D9613_000448 {ECO:0000313|EMBL:KAF4621094.1};
OS Agrocybe pediades.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Agaricineae; Strophariaceae; Agrocybe.
OX NCBI_TaxID=84607 {ECO:0000313|EMBL:KAF4621094.1, ECO:0000313|Proteomes:UP000521872};
RN [1] {ECO:0000313|EMBL:KAF4621094.1, ECO:0000313|Proteomes:UP000521872}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 102.39 {ECO:0000313|EMBL:KAF4621094.1,
RC ECO:0000313|Proteomes:UP000521872};
RA Floudas D., Bentzer J., Ahren D., Johansson T., Persson P., Tunlid A.;
RL Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP +
CC diphosphate; Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666, Rhea:RHEA-
CC COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58045,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78528, ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00048359};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KAF4621094.1}.
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DR EMBL; JAACJL010000015; KAF4621094.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A8H4R0I0; -.
DR Proteomes; UP000521872; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA deacylase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR FunFam; 3.90.740.10:FF:000044; Isoleucine--tRNA ligase; 1.
DR FunFam; 3.40.50.620:FF:000176; Isoleucine-tRNA ligase, putative; 1.
DR FunFam; 1.10.730.10:FF:000004; Isoleucyl-tRNA synthetase, cytoplasmic; 1.
DR FunFam; 3.40.50.620:FF:000023; Isoleucyl-tRNA synthetase,cytoplasmic; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.730.10; Isoleucyl-tRNA Synthetase, Domain 1; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000521872}.
FT DOMAIN 21..642
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 698..853
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1078 AA; 123737 MW; A4C69FDE98125EAE CRC64;
MSFPPHDLST PFDFAKEEEK VIAFWREIDA FQTSLKLSEG RPEYTFYDGP PFATGLPHYG
HLLAGTIKDI VTRHAHVTGH HVSRRFGWDT HGLPVEHEID KRLNITGKDD VLAMGIDKYN
EECRKIVMRY SSEWRQTVER MGRWIDFDND YKTLNLPFME SVWWAFSELF KKGMVYRGLR
VMPYSTGCTT PLSNFEAGQA YKDVNDPAVT VAFPLVDDPS TSLLAWTTTP WTLPSNLALC
VHPDYTYIKI HDTEKDQNFI IHENLLRTLY KDPKKAKFKK VAQFQGSDMK GWRYVPLFEY
FTDQFEDKAF RVLVDTYVTD ADGTGIVHQA PAFGEDDHRI AIAHGVLRPD EMPPCPIDDK
GHFTKEVPDF EGLHVKAADS PIQKYLKAKN RLIVQSTLNH SYPFCWRSGT PLIYRAIPVW
FVKVTPIIDQ LVANNEGTRW VPQNVGDNRF GNWLANARDW NVSRNRYWGT PMPLWVSDDM
EEIVCVGSVE ELEQLSGVKG ITDLHRDKID HITIPSKQGK GVLKRVEEVF DCWFESGSMP
YAQQHYPFEN KELFERTHPA DFVSEGIDQT RGWFYTLLVL STHLFGKAPW KNLIVTGLVL
AADGKKMSKS LKNYPDPNLI IDKYGADATR MFLVNSPIVR GDNLRFREEG VREVISRVLL
PWLNSFRFFM GHVALLKKTT GFDFKYNAHA PLPNNVMDRW ILARCQSLIK LVREEMAAYR
LYTVIPRLLD LVDELTNWYI RFNRKRLKGE DGKEDTVSAL NTLFEALFTL CRIMSSYTPF
ITENLYQSLR TFIPEDPAAG DTRSVHFLSF PEVKEEYFDA EIERKVQRMQ AVIDLGRNVR
ERNNISLKVP LKELLVFHTD PAYLDDVKPL QRYIESELNV RDIVFSSDEA QSGIRYKAVA
DWAVLGRKLR KDLGRVRNAL PAVSSEEIKA YIDTGKIKVD GIELVEGDLT VQRYLELPPS
SEGQFATNTD NDVVIRLDIQ VHAELQGEWL ARELTNRVQK LRKKAGLQAT DDVNVFYRFE
EGTGSDILAA IKDNMDIITK TVGSLPADVK EKAAGSVVII EEEQEIDDVK FLLYLARS
//
