ID   A0A8H5MR73_9HYPO        Unreviewed;       707 AA.
AC   A0A8H5MR73;
DT   19-JAN-2022, integrated into UniProtKB/TrEMBL.
DT   19-JAN-2022, sequence version 1.
DT   02-APR-2025, entry version 11.
DE   RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE            EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN   ORFNames=FNAPI_11509 {ECO:0000313|EMBL:KAF5537130.1};
OS   Fusarium napiforme.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium fujikuroi species complex.
OX   NCBI_TaxID=42672 {ECO:0000313|EMBL:KAF5537130.1, ECO:0000313|Proteomes:UP000574317};
RN   [1] {ECO:0000313|EMBL:KAF5537130.1, ECO:0000313|Proteomes:UP000574317}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NRRL 25196 {ECO:0000313|EMBL:KAF5537130.1,
RC   ECO:0000313|Proteomes:UP000574317};
RA   Kim H.-S., Busman M., Brown D.W., Divon H., Uhlig S., Proctor R.H.;
RT   "Identification and distribution of gene clusters putatively required for
RT   synthesis of sphingolipid metabolism inhibitors in phylogenetically diverse
RT   species of the filamentous fungus Fusarium.";
RL   Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC         EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KAF5537130.1}.
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DR   EMBL; JAAOAO010000546; KAF5537130.1; -; Genomic_DNA.
DR   Proteomes; UP000574317; Unassembled WGS sequence.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR   CDD; cd20335; BRcat_RBR; 1.
DR   CDD; cd22584; Rcat_RBR_unk; 1.
DR   Gene3D; 1.20.120.1750; -; 1.
DR   InterPro; IPR031127; E3_UB_ligase_RBR.
DR   InterPro; IPR002867; IBR_dom.
DR   InterPro; IPR044066; TRIAD_supradom.
DR   PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR   Pfam; PF01485; IBR; 1.
DR   SMART; SM00647; IBR; 2.
DR   SUPFAM; SSF57850; RING/U-box; 1.
DR   PROSITE; PS51873; TRIAD; 1.
PE   4: Predicted;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000574317};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833};
KW   Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT   DOMAIN          215..406
FT                   /note="RING-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51873"
FT   REGION          91..117
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          445..480
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COMPBIAS        91..113
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   707 AA;  82407 MW;  7DEF705DB64A1FAB CRC64;
     MPGAFGPEDY IDFYEGPFEV FNGSGTSREK QEQRASWAVK KLPDLDIVDL DWHKYSPPKA
     LRKAPNITSQ VLLDVLQSSV ENVNARIIEE DRQKKENEEQ RKQEENEKGK SKEPYLPIII
     PTEPPVEEKT NHAVPPPNIK KEILMTTSGP SVVAQADARA NKRRLFALRR FFQRSDERGE
     SSATGAALGA LQERVAEAEH VNDSLGRLTK LNSVEEVECV SCLDDFHPKD VIKVPCHNYC
     RDCFVRLVTA ACQNEQQWPP KCCLNEIPVK TVMRFIPSDL KKTFEDRSKE WELPVSDRVY
     CSNANCNLWI KPKRIYPGKR QGICDRSHVT CTLCRGPAHA EEDCPQDVDM SLTNQLAEDE
     GWKRCFNCNA LVEHREACQH MTCRCGTQFC YVCSRRWRTC TCTMEDLRAL KERVVSRRAE
     RRAQELEEAE ELRHILLQIE EFEHEEALKA ELLRLEQERL EEERRQRELE ERVRQESIRR
     RDIEVKYQEL RTVLGELHDL QQVLLDVDQE EEAENMIIDI RAAKQELEMK HETELSELKA
     LALAKMTEKE NSLNNDFRVR AAKENELEEA YHERLKAYWE GKDNGEQEVE NSMLALRKRM
     DQKHHAWQKW KADQLKVVEA KLEDNRTYRE ELMYSAKHRL DDSCKEKGAE MMRRMAAEKK
     WLEAVILERE KLLNEREAQE VEVDADSMFA VESEDSGDGH YVIATTF
//